In order to investigate the effect of water deficit stress and stress modifiers on some morphological and physiological characteristics and yield of Quinoa, a pots factorial experiment based on a completely randomized design with 16 treatments and 6 replicates was done in 2019 at Urmia University. The first factor includes water deficit stress at four levels, stress at the vegetative growth stage, stress at the reproductive growth stage, stress at seed filling stage and no stress (control). The second factor was foliar spraying of ascorbic acid, salicylic acid, nano-micronutrient chelate fertilizer and Control (water spray). Water deficit stress at different growth stages decreased plant height, a number of laterals, dry weight and fresh weight of vegetative organs, root volume, fresh and dry weight of root and root length compared to control. However, electrolyte leakage and leaf temperature increased. The results showed that water deficit stress at post-anthesis stage decreased grain yield via decreasing 1000-grain weight. Foliar application of growth regulators by improving yield components, increased the yield of quinoa under water deficit stress condition. Therefore, according to the results, it seems that the use of stress modulators in quinoa under water deficit stress with stimulating growth and metabolism, cause to tolorate water deficit stress.