In order to investigate the effect of Arbuscular mycorrhizal fungi in Saffron rhizosphere on yield and nutrient uptake, a study was conducted as split plot factorial based a randomized completely design with three replications in the institute of Soil and Water research in Karaj, Treatment consisted: Irrigation regime as the main factor in three levels (such as complete irrigation as control, water interruption at the beginning of the growing season (mild restriction water) and water interruption at the beginning and mid of the growing season (severe restriction water)), sowing media in three levels (no organic fertilizer, vermicompost (20 ton ha-1) and biochar (10 ton ha-1) and Arbuscular mycorrhizal fungal in three levels (no application, strain a and strain b) as the sub factor. Based on the molecular findings, both strains isolated from saffron rhizosphere belonged to Rhizophagus irregularis. The results of this study showed that the highest fresh yield of flowers, phosphorus and potassium in the leaves with the amount of 7.76 g per pot, 1032.4.4 and 24876.8 mg kg-1, respectively, belonged to the complete irrigation × biochar × strain b treatment. Complete irrigation × vermicompost × strain b treatment had the highest percentage of nitrogen (1.757) in the leaves. The highest fresh and dried yield of stigma (0.358 and 0.062 gr per pot, respectively) was observed in complete irrigation × biochar treatment. Identification of native mycorrhizal fungi and its production as well as the use of organic fertilizers can play an important role in sustainable production of this plant.