Document Type : Research Paper

Author

Assistant Professor, Department of Agriculture, Payame Noor University, Tehran, Iran.

Abstract

In order to investigate the effect of municipal sewage sludge, Azotobacter, and Azospirillum on some nitrogen indices and fatty acid composition of Canola, a factorial experiment has been conducted based on randomized complete block design with three replications in the field of South Tehran Water and Wastewater Company during 2016-2017 and 2017-2018. The experiment is a factorial experiment in a randomized complete block design with three replications. The experimental factors include canola seed inoculation in three levels (without inoculation, inoculation with azotobacter, and inoculation with azospirillum) and the use of municipal sewage sludge in five levels (control, recommended fertilizer, 10, 20, and 30 tons of sewage sludge). Results show that the interaction between seed inoculation and sewage sludge treatments on eicosanoic, palmitic, and arachidonic fatty acids has been significant at 1% and on stearic acid at 5%. Aztobacter and 30 tons of sewage sludge treatment (2680 kg/ha) has had the highest grain yield, 23.5% higher than the control treatment (2170 kg/ha). The highest amount of oleic and linoleic acid is obtained from 30 tons of sewage sludge treatment with 61.23% and 18.41%, respectively. Nitrogen uptake efficiency is the highest in fertilizer recommendation treatment with Azotobacter (16.79%). Interaction effects of inoculation and sewage sludge treatments on both trait consumption efficiency and productivity efficiency (agricultural) have been significant in terms of biomass and grain yield. Results indicate that the use of nitrogen fertilizer in proper amount, provided that it is in a timely manner, combined with inoculation could have a greater effect on productivity efficiency (agricultural) index. It can be stated that with appropriate use of sewage sludge, and necessary precautions, it can be a good substitute for nitrogen fertilizers.

Keywords

Ahmad. A. G., Orabi, S., & Gaballah, A. (2010). Effect of bio-N-P fertilizer on the growth, yield and some biochemical component of two sunflower cultivars. International Journal of Academic Research. 4(2): 271-277.
AOAC. (1990). Method 988.05. Helrich K. (ed.). Official methods of analysis (15th ed.). The Association of Official Analytical Chemists Inc.
Asadi, Gh., Momen, A., Noorzadeh Namaghi, M., & Khorramdel, S. (2013). Effect of Different Levels of Organic and Chemical Fertilizers on Yield and Nitrogen Efficiency Indicators in Pistachio (Plantago ovata Forsk.). Journal of Agricultural Ecology, 5(4) 373 - 382. (In Persian).
Bingham, I. J., Karley, A. J., White, P. J., Thomas, W. T. B., & Russell, J. R. (2012). Analysis of improvements in nitrogen use efficiency associated with 75 years of spring barley breeding. European Journal of Agronomy, 42, 49- 58.
Brechenmacher, L., Kim, M., Benitez, M., Li, M., Joshi, T., Calla, B., Lee, M. P., Libault, M., Vodkin, L. O., Xu, D., Lee, S., Clough, S., & Stancey, G. (2008). Transcription profiling of soybean nodulation by Bradyrhizobium japonicum. Molecular Plant-Microbe Interactions. 21, 631–645. https://doi.org/10.1094/MPMI-21-5-0631.
Dabighi, Kh., Fateh. E., & Aynehband, A. (2016). The Study of Nitrogen Efficiency Indices of Canola (Brassica napus L.) under Different Green Manure Crops and Nitrogen Sources. Iranian Journal of Crop Research, 15(2), 413-424. (In Persian).
Dilsat, B., Konuskan, Mehmet, A., & Abdullah, O. (2019). Physicochemical properties of cold pressed sunflower, peanut, rapeseed, mustard and olive oils grown in the Eastern Mediterranean region. Saudi Journal of Biological Sciences, 26, 340 – 344. https://doi.org/10.1016/j.sjbs.2018.04.005
Dobermann, A., Witt, C., Abdulrachman, S., Gines, H.C., Nagarajan, R., Son, T. T., Tan, P. C., Wong, G. H., Chien, N.V., Thoma, V. T. K., Phung, C. V., Stalin, P., Muthukrishnan, P., Ravi, V., Babu, M., Simbahan, G. C., & dviento, M. A. A., (2003). Soil fertility and indigenous nutrient supply in irrigated rice domains of Asia. Agronomy Journal, 95, 913-922.
Ebrahimghochi, Z., Mohsenabadi, GH., Ehteshami, S. R., & Forghani, A. (2014). Integrated application of different Nitrigen sources on yield and Nitrogen uptake in forage corn (Zea mays L.) in Rasht region. Plant Production Technology, 15(2), 95-107. (In Persian).
Enjalbert, J. N., Zheng, S., Johnson, J. J., Mullen, J. L., Byrne, P. F., & McKay, J. K. (2013). Brassicaceae germplasm diversity for agronomic and seed quality traits under drought stress. Industrial Crops and Products, 47, 176–185. doi: http://dx.doi.org/ 10.1016/j.indcrop.2013.02.037
Fadul, O. (2014). Fatty Acid Content of Seed at Different Development Stages in Canola on Different Soil Types with Low Organic Matter. Plant Production Science, 17(3), 253–259.
Florio, A., Pommier, T., Gervaix, J., Bérard, A., & Le Roux, X. (2017). Soil C and N statuses determine the effect of maize inoculation by plant growth-promoting rhizobacteria on nitrifying and denitrifying communities. Scientific Reports, 7(8411), 1-12. DOI: 10.1038/s41598-017-08589-4.
Gan, Y., Malhi, S. S., Brandt, S., Katepa-Mupondwa, F., & Stevenson, C. (2008). Nitrogen use efficiency and n uptake of juncea canola under diverse environments. Agronomy Journal, 100(2), 285-295.
Giambalvo, D., Ruisi, P. Di., Miceli, G., Frenda, A.S., & Amato, G. (2010). Nitrogen use efficiency and nitrogen fertilizer recovery of durum wheat genotypes as affected by interspecific competition. Agronomy Journal, 102, 707–715
Habibi, Maryam. Majidian, M., & Rabiei, M. (2014). Effects of Elements on Zinc and Sulfur on Grain Yield and Fatty Acid Composition of Canola Oil (Brassica napus L.). Journal of Plant Production Agriculture, 16(1), 69–84. (In Persian).
Javadi, H., Rezvani Moghaddam, P., Rashed Mohassel, M., & Seghat al-Islam, M. (2018). Effect of different levels of nitrogen and green manure on yield and nitrogen use efficiency in purslane. Journal of Plant Production Agriculture, 20(2), 453 - 465. (In Persian).
Keerthi, P., Pannu, R.K., Dhaka, A.K., Daniel, J., & Yogesh, A. (2017). Yield, nitrogen uptake and nutrient use efficiency in Indian mustard (Brassica juncea L.) as effected by date of sowing and nitrogen levels in Western Haryana, India. International Journal of Current Microbiology and Applied Sciences, 6(4), 1168-1177. DOI: 10.20546/ijcmas.2017.604.143
Khajehpour, M. (2005). Industrial plants. Isfahan University Jihad Publications. Isfahan, Iran (In Persian).
Khalid, U., Sarfaraz, Kh. Said, Gh., Muhammad, U. K., Niamatullah, K. M., Anwar, K., & Shad Khan, Kh,. (2012). Sewage Sludge: An Important Biological Resource for Sustainable Agriculture and Its Environmental Implications. American Journal of Plant Sciences, 3, 1708-1721. http://dx.doi.org/10.4236/ajps.2012.312209
Kucheki, A.., Nasiri Mahallati, M., Moradi, R., & Alizadeh, I. (2015). Effect of different levels of nitrogen on yield and nitrogen use efficiency in mixed maize and cotton cultivation. Journal of Agricultural Research, 13(1), 1–13. (In Persian).
Koutroubas, S.D., Antoniadis, V., Fotiadis, S., & Damalas, C.A. (2014). Growth, grain yield and nitrogen use efficiency of Mediterranean wheat in soils amended with municipal sewage sludge. Nutrient Cycling in Agroecosystems, 100, 227–243.
Latar, A.M., Kumar, O., Singh, S.K., & Gupta, A. (2014). Direct and residual effect of sewage sludge on yield, heavy metals content and soil fertility under rice-wheat system. Ecological Engineering, 69, 17–24. doi:10.1016/ j.ecoleng.2014.03.066.
Silva, L. R., Pereira, M. J., Azevedo, J., Mulas, R., Velazquez, E., González-Andrés, F. & Andrade, P. B. (2013). Inoculation with Bradyrhizobium japonicum enhances the organic and fatty acids content of soybean (Glycine max (L.) Merrill) seeds. Food chemistry, 141(4), 3636-3648.
 Mirzashahi, K., & Hosseinpour, M. (2014). Nitrogen fertilizer management on grain yield and nitrogen efficiency indices in maize. Journal of Agriculture (Research & Construction), 102, 31 - 40. (In Persian).
Mohammadi, KH. Ghalavand, A., Aghaalikhani, M., Heidari, G.,Shahmoradi, B., & Sohrabi, Y. (2011). Effect of different methods of crop rotation and fertilization on canola traits and soil microbial activity. Australian Journal of Crop Science, 5(10), 1261-1268.
Nosheen, A., Bano, A., & Ullah, F. (2013). The role of plant growth promoting rhizobacteria on oil yield and biodiesel production of Canola (Brassica napus L.). Energy Sources, 35(A), 1574-1581. DOI: 10.1080/15567036.2010.529561
Oliveira, A. P., Silva, L. R., Andrade, P. B., Valentao, P., Silva, B. M., Gonçalvez, R. F., Pereira, J. A., & Guedes, De. Pinho, P. (2010). Further insight into the latex metabolite profile of Ficus carica. Journal of Agricultural and Food Chemistry, 58, 10855–10863. https://doi.org/10.1021/jf1031185
Poulsen, P., Hasse, B., Jakob, M., Jesper, L., & Andreas, de. N. (2013). Effects of fertilization with urban and agricultural organic wastes in a field trial e Waste imprint on soil microbial activity. Soil Biology & Biochemistry, 57(2013), 794–802. doi:10.1016/j.soilbio.2012.02.031.
Togas, R., Yadav, L. R., Choudhary, S. L., & Shisuvinahalli, G. V. (2017). Effect of Azotobacter on growth, yield and quality of pearl millet. Journal of Pharmacognosy and Phytochemistry, 6(4), 889-891.
Redondo-Cuevas, L., Castellano, G., Torrens, F., & Raikos, V. (2018). Revealing the relationship between vegetable oil composition and oxidative stability: A multifactorial approach. Journal of Food Composition and Analysis, 66, 221-229.
Rezvani Moghaddam, P., Amiri, M. Be., & Sayyedi, S. M. (2014). Effect of Organic and Biological Fertilizers on Yield, Oil Content and Fatty Acid Composition of Sesame Oil (Sesamum indicum L.). Iranian Journal of Crop Sciences, 16(3), 209 - 221. (In Persian).
Rochester, I. J., Peoples, M. B., & Constable, G. A. (2001). Estimation of the N fertiliser requirement of cotton grown after legume crops. Field Crops Research, 70, 43-53.Doi: 10.1016/s0378-4290(00)00150-7
Sharafi, Y., Majidi, M. M., Goli, S. A. H., & Rashidi, F. (2015). Oil Content and Fatty Acids Composition in Brassica Species. International Journal of Food Properties, 18, 2145–2154. DOI: 10.1080/10942912.2014.968284.
Seyed Sharifi, R. (2016). Application of biofertilizers and zinc increases yield, nodulation and unsaturated fatty acids of soybean. Zemdirbyste-Agriculture, 103(3), 251–258. DOI: 10.13080/z-a.2016.103.032
Shakeri, E., Amini Dehghi, M., Tabatabai, S., & Modarres Sanavi, S. (2012). The Effect of Nitrogen Fertilizer and Biofertilizer Containing Azotobacter and Azospirillum on Grain and Fatty Acids Yield of Sesame Cultivars in Yazd. Journal of Crop Research, 10(4), 742–750. (In Persian).
Shehata, M. M., & EL-Khawas, S. A. (2003). Effect of two biofertilizers on growth parameters, yield characters, nitrogenous components, nucleic acids content, minerals, oil content, protein profiles and DNA banding pattern of sunflower yield. Pakistan Journal of Biological Sciences, 6(14), 1257–1268. DOI: 10.3923/pjbs.2003.1257.1268.
Shoghi-Kalkhoran, S., Ghalavand, A., ModarresSanavy, S.A.M. Mokhtassi- Bidgoli, A., & Akbari, P. (2013). Integrated Fertilization Systems Enhance Quality and Yield of Sunflower (Helianthus annuus L.). Journal of Agriculture Sciences and Technology, 15(2013), 1343–1352.
Sorgona, A., Abenavoli, M. R., Gringeri, P. G., & Cacco, G. (2006). A comparison of nitrogen use efficiency definitions in citrus rootstocks. Scientia Horticulturae, 109, 389-393. Doi: 10.1016/j.scienta.2006.06.001
Uribelarrea, M., Moose, S. P., & Below, F. E. (2007). Divergent selection for grain protein affects nitrogen use in maize hybrids. Field Crops Research, 100, 82–90. Doi: 10.1016/j.fcr.2006.05.008
Koutroubas, S. D., Antoniadis, V., Damalas, C. A., & Fotiadis, S. (2020). Sewage sludge influences nitrogen uptake, translocation, and use efficiency in sunflower. Journal of Soil Science and Plant Nutrition, 20, 1912-1922. https://doi.org/10.1007/s42729-020-00262-6
Vatandust, H., Seyed Sharifi, R., Farzaneh, S., & Hassan Panah, D. (2017). Seed filling and composition of some rapeseed (Brassica napus L.) fatty acids using biofertilizers and cut irrigation. Journal of Agricultural Knowledge and Sustainable Production, 72(4), 23–37. (In Persian).
Woźniak, E., Waszkowska, E., Zimny, T., Sowa, S., & Twardowski, T. (2019). The rapeseed potential in Poland and Germany in the context of production, legislation, and intellectual property rights. Frontiers in Plant Science, 10, 1423. 5(November 2019).   https://doi.org/10.3389/fpls.2019.01423.
Zhang, D., Li, W., Xin, C., Tang, W., Eneji, A.E., & Dong, H. (2012). Lint yield and nitrogen use efficiency of field-grown cotton vary with soil salinity and nitrogen application rate. Field Crops Research, 138, 63–70. http://dx.doi.org/10.1016/j.fcr.2012.09.013
Zhang, Q., Hu, J., Lee, D-J., Chang, Y., & Lee, Y-J. (2017). Sludge Treatment: Current Research Trends, Bioresource Technology, 243, 1159–1172. http://dx.doi.org/10.1016/j.biortech.2017.07.070