In order to investigate the effect of municipal sewage sludge and Azotobacter and Azospirillum on some nitrogen indices and fatty acid composition, an experiment was conducted in the field of south of Tehran in Water and Wastewater Company during 2016-2017 and 2017-2018 Crop years. The experiment was conducted as a factorial experiment in a randomized complete block design with three replications. Experimental factors include canola seed inoculation (control, inoculation with azotobacter, inoculation with azospirillum) and The use of municipal sewage sludge (control, recommended fertilizer, 10, 20 and 30 tons of sewage sludge). The results showed that the interaction between seed inoculation and sewage sludge treatments on eicosanoic, palmitic and arachidonic fatty acids was significant at 1% . The highest amount of oleic and linoleic acid was obtained from 30 tons of sewage sludge treatment with 61.23 and 18.41 Percent, respectively. Nitrogen uptake efficiency was highest in fertilizer recommendation treatment with Azotobacter (16.79 Percent) . Interaction effects of inoculation and sewage sludge treatments on both trait consumption efficiency and productivity efficiency (agricaltural) were significant at (p ≤ 0.01) in terms of biomass and grain yield. The results indicate that the use of nitrogen fertilizer in proper amount and provide its in a timely manner Combined with inoculation, it could have a greater effect on the Productivity efficiency (agricaltural) index. It can be stated that with the appropriate use of sewage sludge, with the necessary precautions, it can be a good substitute for nitrogen fertilizers.