Document Type : Research Paper


Assistant Professor, National Salinity Research Center, Agricultural Research, Education and Extension Organization (AREEO), Yazd, Iran


In order to investigate the effect of glycine betaine (GB) on some growth and yield traits of Damask Rose, an experiment was conducted as factorial based on a completely randomized design with 3 replications at Research Greenhouse of National Salinity Research Center during 2018-19 growing season. The treatments were four levels of salinity: 2 (control), 5, 8 and 11 dS/m and three levels of GB: without application (control), spraying with 50 mM and 100 mM concentrations. The results showed that the main effect of salinity stress was significant on all studied characteristics, except branch number per plant and petal number. Moreover, the main effect of GB was significant on all measured traits, except branch number per plant, petal number, height and petal weight/flower weight ratio. The most flower number per plant, flower yield and essential oil yield were observed in stressed plants at 2, 5 and 8 dS/m salinity, had more 7.5 flower, 29.5 g flower weight per plant and 6 mg essential oil per plant than 11 dS/m, respectively. The interaction results between salinity stress and foliar application of glycine betaine showed that the treated plants with 2 dS/m and 100mM GB had the highest height (71 cm) and the treated plants with 10 dS/m and without application of GB had the lowest height (40 cm). In general, using 50 mM GB and without salinity stress caused the highest Damask Rose weight.


Abdelmotlb, N.A., Abdel-All, F.S., Abd EL-Hady S.A., EL-Miniawy, S.M. & Ghoname, A.A. (2019). Glycine betaine and sugar beet extract ameliorated salt stress adverse effect on green bean irrigated with saline water. Middle East Journal of Applied, 9(1), 142-154.
Adak, N. (2019). Effects of glycine betaine concentrations on the agronomic characteristics of strawberry grown under deficit irrigation conditions. Applied Ecology and Environmental Research, 17(2), 3753-3767.
Alasvandyari, F., Mahdavi, B. & Madah Hosseini, S. (2017). Glycine betaine affects the antioxidant system and ion accumulation and reduces salinity-induced damage in safflower seedlings. Archives of Biological Sciences, 69(1), 139-147.
Ali, E.F., Bazaid, S.A. & Hassan, F.A.S. (2014). Salinity tolerance of taif roses by gibberellic acid (GA3). International Journal of Science and Research, 3(11), 184-192.
Annunziata, M.G., Ciarmiello, L.F., Woodrow, P., Dell’Aversana, E. & Carillo, P. (2019). Spatial and temporal profile of glycine betaine accumulation in plants under abiotic stresses. Frontiers in Plant Science, 10, 1-13.
Armin, M. & Miri, H.R. (2014). Effects of glycine betaine application on quantitative and qualitative yield of Cumin under irrigated and rain-fed cultivation. Journal of Essential Oil Bearing Plants, 17(4), 708-716.
Asgari, F., Motarashaezadeh, B. & Kalatehjari, S. (2017). Investigation the effect of silicon on morphological and physiological characteristics of (Rosa chinensis var.minima) Meillandina prince. 4th Internatonal conference on applied research in chemistry science and biology, p. 12. (in Persian)
Baniasadi, F., Safari, V. & Maghsoudimod, A. A. (2015). Effect of putrescine and salinity on morphological and biochemical traits and pigment content of marigold plant (Calendula officinalis L.). Journal of Science and Technology of Greenhouse Culture Soilless Culture Research Center, 6(1), 125-134. (in Persian)
Cabrera, R.I. (2003). Demarcating salinity tolerance in greenhouse roses. Acta Horticulture, 609, 51-57.
  Cabrera, R.I. & Perdomo, P. (2003). Reassessing the salinity tolerance of greenhouseroses under soilless production conditions. HortScience, 38, 533-536.
Cai, X., Niu, G., Starmana, T. & Hall, C. (2014). Response of six garden roses (Rosa × hybrida L.) to salt stress. Scientia Horticulturae, 168, 27-32.
Cha-Um, S. & Kirdemanee, C. (2015). In Vitro Flowering of Miniature Roses (Rosa x hybrida L. 'Red Imp') in Response to Salt Stress. European Journal of Horticultural Science, 75(6), 239-245.
Dehghan, A. & Rahimmalek, M. (2018). The effect of salt stress on morphological traits and essential oil content of Iranian and foreign yarrow (Achillea millefolium L.) genotypes. Journal of Science and Technology of Greenhouse Culture, 9(2), 23-38. (in Persian)
Ebrahimi, M. & Sharifzadegan, H. (2016). Introduce of principles of Golmohammadi planting. Agricultural Research, Education and Extension Organization (AREEO), Ministry of Agriculture-Jihad, p. 20. (in Persian)
Einset, J. & Connolly, E.L. (2009). Glycine betaine enhances extracellular processes blocking ROS signaling during stress. Plant Signaling & Behavior, 4(3), 197-199.
Giri, J. (2011). Glycinebetaine and abiotic stress tolerance in plants. Plant Signaling & Behavior, 6(11), 1746-1751.
Ibrahim, A.H. (2004). Efficacy of exogenous glycine betaine application on sorghum plants grown under salinity stress. Acta Botanica Hungarica, 46(3), 307-318.
Kadkhodaie, H., Sodaeizadeh, H., Mosleh arani, A. (2014). The effects of exogenous application of glycine betain on growth and some physiological characteristics of Brossica napus under drought stress in field condition. Desert Ecosystem Engineering Journal, 3(4), 79-90.
Kaya, C., Sonmez, O., Aydemir, S., Dikilitas, M. (2013). Mitigation effects of glycinebetaine on oxidative stress and some key growth parameters of maize exposed to salt stress. Turkish Journal of Agriculture and Forestry, 38, 188-194.
Khayarbadi, E. & Armin, M. (2014). Salinity stress mitigation by glycin in peppermint (Boago officinalis.). M.Sc. dissertation. Faculty of Agriculture and Veterinary. Islamic Azad University of Sabzevar Branch Iran. (in Persian)
Li, X., Wan, S., Kang, Y., CHen, X. & Chu, L. (2016). Chinese Rose (Rosa chinensis) Growth and Ion Accumulation un- der Irrigation with Waters of Different Salt Contents. Agricultural Water Management, 163, 180-189.
Masumi, Z., Zandi, P. & Tabaei Aghdaei, S.R. (2014).The quantity and quality of essential oil, yield and yield components of seven genotypes of rose (Rosa damascene Mill.) in Fars Province. Iranian Journal of Medicinal and Aromatic Plants, 30(1), 186-197. (in Persian)
Moghbeli Mehni Dareroodi, A., Delbari, M. & Koohi, N. (2016). Investigation of Vegetative and Reproductive Characteristics of Damask Rose in Different Irrigation Regimes through Surface and Subsurface Drip Irrigation. Iranian Journal of Soil and Water Research, 46(4), 673-683. (in Persian)
Mortezainajad,‌‌ F‌‌.,‌‌ Khavarinajad, R.A. & Emami, M. (2005). Evaluation of some performance parameters and proline rice varieties under salt stress. New Agricultural Science, 2(4), 65-70. (in Persian)
Murmu, K., Murmu, S., Kumar Kundu, C. & Sekhar Bera, P. (2017). Exogenous proline and glycine betaine in plants under stress tolerance. International Journal of Current Microbiology and Applied Sciences, 6(9), 901-913.
Nazarolmolk, E., Zahedi, B. & Zeinali, H. (2017). Relations between flower yield and its components in 10 genotypes of damask rose in Golpayegan. Iranian Journal of Horticultural Science, 48(2),243-249. 10.22059/ijhs.2017.127919.801
Safi, M., Fardous, A., Muddaber, M., EL-Zuraiqi, S., AL-hadidi, L. & Bashabshehah, I. (2005). Effect of Treated Saline Water on Flower Yield and Quality of Roses Rosa hybrida and Carnation Dianthus caryophyllus. ScienceAsia, 31, 335-339.
Sakamoto, A. & Murata, N. (2002). The role of glycine betaine in the protection of plants from stress: clues from transgenic plants. Plant, Cell & Environment, 25, 163-171.
Shahbani, Z., Khosh-Khui, M., Salehi, H., Kafi, M., Kamgar Haghighi, A.A. & Eshghi, S. (2018). Effects of salinity stress on morphological and physiological characteristics of miniature rose (Rosa chinensis Jacq. var. minima Rehd.), Iranian Journal of Horticultural Science and Technology, 19(1), 41-52. (in Persian)
Showler, A.T. & Castro, B.A. (2010). Influence of drought stress on Mexican rice borer (Lepidoptera: Crambidae) oviposition preference in sugarcane. Crop Protection, 28, 722-727.
Sobahan, M.A., Akter, N., Murata, Y. & Munemasa, S. (2016). Exogenous proline and glycinebetaine mitigate the detrimental effect of salt stress on rice plants. Silpakorn University Science and Technology Journal, 10(3), 38-43.
Tabaei-aghdaei, S.R., Rezaee, M.B. & Jaymand, K. (2003). Evaluate the variation in components flowers and essential oil yield Damask rose genotypes in Kashan. Iranian Rangelands and Forests Plant Breeding and Genetic Research, 11(2), 219-234. (in Persian)
Vadizadeh, P., Sarajoughi, M. & Mir Taheri, S.M. (2017). Study of salicylic acid and glycine effect on some agronomic traits of Alfalfa under wet stress conditions. Journal of Agronomy and Plant Breeding, 13(2), 2-14. (in Persian)
Yildirim, E., Ekinci, M., Turan, M., Dursun, A., Kul, R. & Parlakova, F. (2015). Roles of glycine betaine in mitigating deleterious effect of salt stress on lettuce (Lactuca sativa L.). Archives of Agronomy and Soil Science, 61(12), 1673-1689. 10.1080/03650340.2015.1030611.