Document Type : Research Paper


1 Instructor, Horticulture Crops Research Department, Agricultural and Natural Resources Research Center of Semnan Province (Shahrood), AREEO, Shahrood, Iran

2 Assistant Professor, Department of Horticulture, Agriculture Faculty, Ferdowsi University of Mashhad, Mashhad, Iran.

3 Professor, Department of Horticulture, Agriculture Faculty, Ferdowsi University of Mashhad, Mashhad, Iran

4 Associate Professor, Horticulture Crops Research Department, Agricultural and Natural Resources Research Center, Khorasan Razavi, AREEO, Mashhad, Iran


In order to study the role of fruit tree rootstocks on nutrient content in leaves of two Iranian red flesh apple genotypes and to determine the correlation of nutrients with different secondary metabolites, this study was conducted in Research Garden of Horticultural Sciences of Ferdowsi University of Mashhad during 2017-2019. The rootstocks that used were two dwarfing rootstocks, M9 and B9 and one red flesh apple colony rootstock of Bekran which were tested in a factorial randomized complete block design. The results of this study showed that the rootstock had a significant effect on nitrogen and phosphorus content in leaf samples of two red flesh apple genotypes and Red delicious cultivar, also, rootstock, scion and their interaction had a significant effect on potassium, iron, calcium and zinc content of leaf samples. The effect of B9 on the amount of all elements of scion leaves was negative and the effect of M9 was positive in all cases. The results showed that PAL enzyme activity was positively correlated with nitrogen, potassium, calcium, iron and zinc. Anthocyanin synthesis was also positively correlated with nitrogen content (0.47). The results showed that the amount of potassium, calcium and iron were highly correlated with UFGT activity. Total flavonoid content was also significantly correlated with calcium and zinc (-0.64 and -0.45, respectively) that these effects could be due to the participation of some of these nutrients as cofactors in enzyme activity and or precursors of phenolic compounds and secondary metabolites.


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