Document Type : Research Paper


1 Associate Professor, Department of Agronomy, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

2 Assistant Professor, Department of Horticultural Sciences, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.

3 Ph.D. Student , Department of Agronomy, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran


In order to investigate the effect of biochar and biological fertilizer on photosynthetic pigments, yield and nutrient content of peppermint (Mentha piperita L.), an experiment was conducted as factorial based on a randomized complete block design in three replications at Sari Agricultural Sciences and Natural Resources University in 2017. Treatment consisted biochar on two level (0 and 10 ton ha-1) and organic and biological fertilizers on five level (no fertilizer (F0), vermicompost (10 ton ha-1) (F1), Pseudomonas fluorescens (F2), Azotobacter (F3) and Arbuscular mycorrhizal (Glomus mosseae, G. intradicces and G. etunicatum) (F4)). The results of this study showed that the main effect of biochar and bio-fertilizer had a significant effect on leaf, stem and total fresh weight of the plant. In addition, the use of biochar and also mycorrhiza treatment increased average of leaf fresh weight (294.73 and 306.8 g.m-2), stem fresh weight (366.73 and 361.15 g.m-2) and total fresh weight (661.46 and 667.95 g.m-2). The interaction effect of treatments had a significant effect on leaf, stem and total dry weight. The highest rate of these traits was observed in biochar treatment with mycorrhiza, which had no significant effect with by a combination of vermicompost + biochar. Therefore, with respect to the production of medicinal plants in the low-input cropping systems, environmental protection, and achievement of sustainable agricultural to improve plant growth and photosynthesis of peppermint application of biochar + mycorrhiz and biochar + vermicompost treatment is recommended.


Anderson, C.R., Condron, L.M., Clough, T.J., Fiers, M., Stewart, A., Hill, R.A. & Sherlock, R.R. (2011). Biochar induced soil microbial community change: Implications for biogeochemical cycling of carbon, nitrogen and phosphorus. Pedobiologia, 54(5-6), 309- 320.
Arjmand Alavi, M., Hatamzadeh, A. & Ehteshami, S.M.R. (2014). Effect of bulb inoculation with four species mycorrhizal fungi on quantitative and qualitative yield of two lily species. Seed Science and Research, 1(2), 57-65. (in Persian)
Ayyobi, H., Peyvast, G.A. & Olfati, J.A. (2013). Effect of vermicompost and vermicompost extract on oil yield and quality of peppermint (Mentha piperita L.). Journal of Agricultural Sciences, 58(1), 51-60. (in Persian)
Badr, L.A.A. & Fekry, W.A. (1998). Effect of intercropping and doses of fertilization on growth and productivity of taro and cucumber plants. 1-Vegetative growth and chemical constituents of foliage. Zagazig Journal of Agricultural Research, 25, 1087-1101.
Bamminger, C., Marschner, B. & Juschke, E. (2014). An incubation study on the stability and biological effects of pyrogenic and hydrothermal biochar in two soils. European Journal of Soil Science, 65(1), 72-82.
Behnam, H., Farrokhian Firouzi, A. & Moezzi, A.A. (2016). Effect of biochar and compost sugarcane bagasse on some soil mechanical properties. Water and Soil Conservation, 23(4), 235-250. (in Persian)
Casson, S.A. & Lindsey, K. (2003). Genes and signalling in root development. New Phytologist, 158(1), 11-38.
Darzi, M.T., Haj Seyd Hadi, M.R. & Rejali, F. (2012). Effects of cattle manure and biofertilizer application on biological yield, seed yield and essential oil in coriander (Coriandrum sativum). Medicinal Plants, 2(42), 77-90. (In Persian).
Dharmakeerthi, R.S., Chandrasiri, J.A.S. & Edirimanne, V.U. (2012). Effect of rubber wood biochar on nutrition and growth of nursery plants of Hevea brasiliensis established in an Ultisol. SpringerPlus, 1(84), 1-12.
Divband Hafshejani, L., Naseri, A., Hooshmand, A., Abbasi, F. & Soltani Mohammadi, A. (2017). Effect of sugarcane bagasse biochar application on chemical properties a sandy loam soil. Irrigation Sciences and Engineering, 40(1), 63-72. (in Persian)
Faraji Mehmani, A., Esmaielpour, B., Sefidkon, F., Abbaszadeh, B., Khavazi, K. & Ghanbari, A.R. (2014). Effects of biofertilizers on growth criteria, quantitative and qualitative yield of summer savory (Satureja hortensis L.). Agroecology, 6(4), 870-879. (in Persian)
Gharib, A.F., Moussa, L.A. & Massoud, O.N. (2008). Effect of compost and bio-fertilizers on growth, yield and essential oil of sweet marjoram (Majorana hortensis) plant. International of Journal Agriculture and Biology, 10(4), 381-387.
Hatami, S.F., Esmaielpour, B., Hadian, J., Khavazi, K., Soltani Toolarood, A.A. & Abbaszadeh-dahaji, P. (2014). Effects of biofertilizers on morphological traits of tarragon. Soil Biology, 2(1), 55-63. (in Persian)
Hejazi Zadeh, A., Gholam AliZadeh Ahangar, A. & Ghorbani, M. (2016). Effect of biochar on lead and cadmium uptake from applied paper factory sewage sludge by sunflower (Heliantus annus L.). Water and Soil Science, 26(2), 259-271. (in Persian)


Jeffries, P., Gianinazzi, S., Perotto, S., Turnau, K. & Barea, J.M. (2003). The contribution of arbuscular mycorrhizal fungi in sustainable maintenance of plant health and soil fertility. Biology and Fertility of Soils, 37(1), 1-16. DOI 10.1007/s00374-002-0546-5
Johnson, C.R. (1984). Phosphorus nutrition on mycorrhizal colonization, photosynthesis, growth and nutrient composition of Citrus aurantium. Plant and Soil, 80(1), 35-42.
Khalilidarini, K., Armin, M. & Marvi, H. (2014). The effect of the amount and frequency of complete fertilizer foliar application on quantitative and qualitative yield of peppermint (Mentha piperita). Crop Science Research in Arid Regions, 1(1), 85-100.(in Persian)
Liu, H., Tang, Y., Nell, M., Zitter-Eglseer, K., Wawscrah, C., Copp, B., Wang, S. & Novak, J. (2014). Arbuscular mycorrhizal fungal colonization Glycyrrhiza glabra roots enhances plant biomass, phosphorus uptake and concentration of root secondary metabolites. Journal of Arid Land, 6(2), 186-194.
Mahmoudzadeh, M., Rasouli Sadaghiani, M.H. & Asgari Lajayer, H. (2016). Effect of plant growth promoting rhizobacteria and arbuscular mycorrhizal fungi on growth characteristics and concentration of macronutrients in peppermint (Mentha pipperita L.) under greenhouse conditions. Journal of Science and Technology of Greenhouse Culture Soilless Culture Research Center, 6(4), 155-168. (in Persian)
Maleki, V., Salimi, KH. & Ardakani, M.R. (2016). Effect of biofertilizers on vegetative growth and essential oil of sweet basil. Agroecology, 11(4), 49-56. (in Persian)
Mukherjee, A. & Zimmerman, A.R. (2013). Organic carbon and nutrient release from a range of laboratory-produced biochars and biochar-soil mixtures. Geoderma, 193(1), 122-130.
Mousavi, F., Solymani, A., Rezaiinezhad, A.H. & Kheyri, A.A. (2014). The effect of vermicompost on some quantitative and qualitative properties of peppermint. The First National Conference on New Ideas in Sustainable Agriculture,March 5, Boroujerd, Islamic Azad University. (in Persian)
Nur, M.S.M., Islami, T., Handayanto, E., Nugroho, W.H. & Utomo, W.H. (2014). The use of biochar fortified compost on calcareous soil of east Nusa Tenggara, Indonesia: 2. Effect on the yield of maize (Zea mays L.) and phosphate absorption. American-Eurasian Journal of Sustainable Agriculture, 8(5), 105-111.
Ortas, I., Sari, N., Akpinar, Ç. & Yetisir, H. (2011). Screening mycorrhiza species for plant growth, p and zn uptake in pepper seedling grown under greenhouse conditions. Scientia Horticulturae, 128(2), 92-98. DOI: 10.1016/j.scienta.2010.12.014
Pirasteh Anosheh, H., Emam, Y. & Jamali Ramin, F. (2010). Comparative effect of biofertilizers with chemical fertilizers on sunflower (Helianthus annuus L.) growth, yield and oil percentage in different drought stress levels. Agroecology, 2(3), 492-501. (in Persian)
Porra, R.J. (2002). The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b. Photosynth Research, 73(1-3), 149-156.
Pooyanfar, M., Norafkan, H., Ehsasi, H. & Mahmoodi Rad, Z. 2017. The effect of foliar application of vermicompost tea on morphological characteristics and yield components of peppermint. Third International Conference on Sustainable Development, Solutions and Challenges Focusing on Agriculture, Natural Resources, Environment and Tourism. March 9, Tabriz, Iran. (in Persian)
Ratti, N., Kumar, S., Verma, H.N. & Gautam, S.P. (2001). Improvement in bioavailability of tricalcium phosphate to Cymbopogon martinii var. motia by rhizobacteria, AMF and Azospirillum inoculation. Microbiological Research, 156(2), 145-149.
Rezaeeyan, A. (2014). Effect of biochar and arbuscular mycorrhiza on cadmium absorption, transfer and accumulation in peppermint. Thesis, Shahroud University, Semnan Province, Iran. (in Persian)
Sajadinik, R. & Yadavi, A.R. )2013(. Effect of nitrogen fertilizer, vermicompost and nitroxin on growth indices, phenological stages and grain yield of sesame. Crop Production, 6(2), 73-100. (in Persian)
Salehi Sardoei, A. (2014). Vermicompost effects on the growth and flowering of marigold (Calendula officinalis). European Journal of Experimental Biology, 4(1), 651-655.
Seifi, S.M., Kashani, A., Ardakani, M.R., Rejali, F., Timajchi, M. & Abasian, M. (2011). The effect of different species of mycorrhiza on phytohormone changes in soybean (Glycine max L.) by nano, biologic and chemical fungicides. Agroecology, 3(2), 254-264. (in Persian)
Stajkovic, O., Delic, D., Josic, D., Kuzmanovic, D., Rasulic, N. & Knezevic-Vukcevic, J. (2011). Improvement of common bean growth-promoting bacteria. Romanian Biotechnological Letters, 16(1), 5919-5926.
Steinbeiss, S., Gleixner, G. & Antonietti, M. (2009). Effect of biochar amendment on soil carbon balance and soil microbial activity. Soil Biology and Biochemistry, 41(6), 1301-1310.
Theunissen, J., Ndakidemi, P.A. & Laubscher, C.P. (2010). Potential of vermicompost produced from plant waste on the growth and nutrient status in vegetable production. International Journal of Physical Sciences, 5(13), 1964-1973.
Vanek, S.J. & Lehmann, J. (2015). Phosphorus availability to beans via interactions between mycorrhizas and biochar. Plant Soil, 395(1-2), 105-123.
Watzinger, A., Feichtmair, S., Kitzler, B., Zehetner, F., Kloss, S., Wimmer, B., Boltenstern, S.Z. & Soja, G. (2014). Soil microbial communities responded to biochar application in temperate soils and slowly metabolized 13C-labelled biochar as revealed by 13C PLFA analysis: results from a short term incubation and pot experiment. European Journal of Soil Science, 65(1), 40-51.
Westerman, R.E.L. (1990). Soil testing and plant analysis. Soil Science Society of America.
Wu, S.C., Cao, Z.H., Li, Z.G., Cheung, K.C. & Wong, M.H. (2005). Effects of biofertilizer containing n-fixer, p and k solubilizers and AM fungi on maize growth: a greenhouse trial. Geoderma, 125(1-2), 155-166.
Yaghoby, M., Amerian, M. & Asghar, H. (2014). Comparison of the effect of biological and biofertilized fertilizers on some physiological traits in bean growing. The 2nd National Conference of the Desert with the Approach of the Management of Arid and Desert Areas. Semnan University. (in Persian)
Zargari, A. (1996). Medicinal Plants. University of Tehran Publications, Iran. (in Persian)