Document Type : Research Paper


1 Assistant Professor, Medicinal Plants Research Center, Shahed University, Tehran, Iran.

2 Former M.Sc. Student, Green Space of Tehran Municipality, Tehran, Iran.

3 Former M.Sc. Student, Medicinal Plants Research Center, Shahed University, Tehran, Iran.


In order to investigate the morpho-physiological responses of Purslane plant to methyl jasmonate under salinity stress a split plot based on a completely randomized design with two factors and three replications was carried out in medicinal plants research center, Shahed University, Tehran during 2017. The factors were, salinity with four levels (0, 3, 6 and 9 dS/m) as a main factor and methyl jasmonate with four levels (0, 0.25, 0.5 and 0.75 mM) as a sub factor. The results showed that by increasing salinity levels, the growth indices such as root length, fresh weight of root, chlorophyll b, total chlorophyll and the amount of superoxide dismutase decreased, while by increasing the salinity levels the proline content and the activity of catalase and MDA in the leaf increased. Applying methyl jasmonate under salinity stress reduced the growth indices and photosynthetic pigments. The highest number of branches, number of leaves and chlorophyll b was obtained at 6 dS/m salinity and 0.5 mM methyl jasmonate. By increasing methyl jasmonate level, the proline content and the activity of CAT increased, while the amount of MDA and SOD enzymes decreased. Therefore, it can be concluded that the is the tolerance of Portulaca oleracea plant to salinity was up to six dS/m, and consuming low amounts of methyl jasmonate can improve yield and physiological indices in Purslane.


Aebi, H.E. (1984). Catalase. In Method of Enzymatic analysis, VCH, Weinheim, Germany-Deerfield, FL. 3: 273-286.
Bates, L.S. Waldren, R.P. & Teare, I.D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil. 39(1): 205-207.
Beauchamp, C. & Fridovich, I. (1971). Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry. 44: 276-287.
Brouki-Milan, E. Hassni, L. Abdollahi-Mandoulakani, B. Darvishzadeh, R. Kheradmand, F. & Hassani, A. (2016). The effect of different concentrations of methyl jasmonate on the activity of antioxidant enzymes and total protein in basil. Journal of Crop Improvement. 18(1): 103-115
Cayley, S. Lewis, B.A. & Record, M.T. (1992). Origins of the osmoprotective properties of betaine and proline in Esherichia coli K-12. Journal of Bacteriology. 174: 1586-1595.
Garratt, L.C. Janagoudar, B.S. Lowe, K.C. Anthony, P. Power, J.B. & Davey, M.R. (2002). Salinity tolerance and antioxidant status in cotton cultures. Free Radical Biology and Medicine. 33(4): 502-511.
Goyal, S.H. & Ramawat, K.G. (2008). Ethrel treatment enhanced isoflavonoids accumulation in cell suspension cultures of Pueraria tuberosa, a woody legume. Acta Physiologiae Plantarum. 30(6): 849-853.
Hayat, Q. Hayat, S. Irfan, M. & Ahmad, A. (2010). Effect of exogenous methyl jasmonate under changing environment: A review. Environmental and Experimental Botany. 68: 14-25.
Heath, R.L. & Packer, L. (1968). Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of biochemistry and biophysics. 125(1): 189-198.
Hildmann, T. Ebneth, M. Peña-Cortés, H. Sánchez-Serrano, J.J. Willmitzer, L. & Prat, S. (1992). General roles of abscisic acid and jasmonic acid in gene activation as a result of mechanical damage. Plant Cell. 4: 1157-1170.
Holm, L.G. Plunkett, D.L. Pancho, J.V. & Herberger, J.P. (1977). The world's worst weeds - distribution and biology. University Press of Hawaii, Honolulu. 609 pp.
Jaleel, C.A., Gopi, B., Sankor, P., Manivannaon, A., Kishorekumar, R.S. & Panneers, L. (2007). Studies on germination, seedling vigner, lipid peroxidatoin and proline metabolism in Catharathus roseus seedling under salt stress. South African Jolurnal of Botany. 73 (2): 190-195.
Katsuhara, M. Otsuka, T. & Ezaki, B. (2005). Salt stress induced lipid peroxidation is reduced by glurathione S and Tran frease, But this reduction of lipid proxides is not enough for a recovery of root growth in Arabidopsis. Plant Sciences. 169(2): 369-373.
Kiarostami, K.H. Mohseni, R. & Saboora, A. (2010). Biochemical changes of Rosmarinus officinalis under salt stress. Journal of Stress Physiology and Biochemistry. 6(3): 114-122
Kim, H.J. Chen, F. Wang, X. & Rajapakse, N.C. (2006). Effect of on secondary metabolites of sweet basil (Ocimum basilicum L.). Journal of Agriculture and Food Chemistry. 54(6): 2327-2332.
Koca, H. Bor, M. Ozdemir, F. & Turkan, I. (2007). The effect of salt stress on lipid peroxidation, antioxidative enzymes and proline content of sesame cultivars. Environmental and Experimental Botany. 60(3): 344-351
Kovacik, J. Backor, M. Strnad, M. & Repcak, M. (2009). Methyl jasmonate-induced changes to growth and phenolic metabolism in Matricaria chamomilla plants. Plant Cell Reports. 28: 135-143.
Lichtenthaler, H.K. (1987). Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods in enzymology. 148: 350-382.
Masoodi, M.H. Ahmad, B. Mir, S.R. Zargar, B.A. & Tabasum, N. (2011). Portulaca oleracea L. A review. Journal of Pharmacy Research. 4(9): 3044-8.
Molassiotis, A. Sotiropoulos, T. Tanou, G. Diamantidis, G. & Therios, I. (2006). Boron-induced oxidative damage and antioxidant and nucleolytic responses in shoot tips culture of the apple rootstock EM 9 (Malus domestica Borkh). Environmental and Experimental Botany. 56(1): 54-62.
Muhammad, Z. & Hussain, F. (2010). Vegetative growth performance of five medicinal plants under NaCl salt stress. Pakistan Journal of Botany.  42(1): 303-316.‏
Noctor, G. & Foyer, C.H. (1998). Ascorbate and glutathione: Keeping active oxygen under control. Annual Review Plant Physiology Plant Molecular Biology. 49: 249-279.
Pasandi-Pour, A., Farahbakhsh, H., Saffari, M. & Karamat, B. (2013). The effect of salicylic acid on some physiological reactions of fenugreek (Trigonella foenum-graecum) under salinity stress. Journal of Crop Ecophysiology. 7-2(26): 215-228.
Qureshi, A.S. Qadir, M. Heydari, N. Turral, H. & Javadi, A. (2007) A review of management
strategies for salt-prone land and water resources in Iran. Colombo, Sri Lanka: International Water Management Institute. 30p.
Rahimi-Tashi, T. & Niknam, V. (2015). Evaluation of methyl jasmonate pretreatment and salinity stress on some physiological and biochemical parameters in Triticum aestivum L. Iranian Journal of Plant Biology. 28(2): 297-306.
Rajeshwari, V. & Bhuvaneshwari, V. (2017). Methyl jasmonate Induced Salt Stress Tolerance in Plants. International Journal of Plant Biology and Research. 5(3): 1-6.
Salimi, F. Shekari, F. & Hamzei, J. (2014). Effect of Salinity Stress and Foliar Application of Methyl Jasmonate on Photosynthetic Rate, Stomatal Conductance, Water Use Efficiency and Yield of German Chamomile. Iranian Journal of Field Crops Research. 12(2): 328-334. 
Santos-Soares, A.M.A. Souza, T.F. Jacinto, T. & Machado, O.L.T. (2010). Effect of Methyl Jasmonate on antioxidative enzyme activities and on the contents of ROS and H2O2 in Ricinus communis leaves. Brazilian Society of Plant Physiology. 22(3): 151-158.
Thiem, B. & Krawczyk, A. (2010). Enhanced isoflavones accumulation in treated in vitro cultures of kudzu (Pueraria lobata Ohwi). Herba Polonica. 56(1): 48-56.
Turan, M.A. Turkmen, N. & Taban, N. (2007). Effect of NaCl on stomatal resistance and proline, chlorophyll, Na, Cl and K concentrations of lentil plants. Journal of Agronomy. 6(2): 378-381.
Zahir, M. & Farrukh, H. (2010). Effect of NaCl salinity on the germination and seedling growth of some medicinal plants. Pakistan Journal of Botany. 42(2): 889-897 
Zargari, A. (1997). Medicinal Plants. University Press of Tehran, Tehran. 1010pp
Zarghami-Moghaddam, M. Shoor, M. Ganjeali, A. Moshtaghi, N. & Tehranifar, A. (2014). Effect of methyl jasmonate on morphological and Ornamental characteristics of Petunia hybrida at drought stress. Indian Journal of Fundamental and Applied Life Sciences. 4 (3): 523-532.
Zhao, J. Davis, L. & Verpoorte, R. (2005). Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnology Advances. 23(4): 283-333.