Document Type : Research Paper


1 M.Sc. Student, College of Plant Production, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran.

2 Research Assistant Professor, Department of Agronomy, College of Plant Production, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran.

3 M.Sc. Student, Department of Agronomy, College of Plant Production, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran.

4 Assistant Professor, Department of Agronomy, College of Plant Production, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran.

5 . Instructor, Department of Plant Protection, College of Plant Production, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran.


This study was conducted to investigate the effects of planting date, foliar application of benomyl fungicide and potassium silicate in improving the quality of soybean (cv. Williams) seed in Gorgan during 2011 and 2012. In the first year (2011), the experimental treatments were including planting dates (April 20, May 13, June 3, June 29 and July 22) and foliar application of benomyl fungicide (used at R3 + R6 or unused) and in the second year (2012) were including planting dates (May 1 and July 7), foliar application of benomyl fungicide, silicon (Si) and silicon + benomyl. Water-soluble potassium silicate as silicon supply, in three stages (V6, R1 and R4), at the concentration of 40 grams per liter was sprayed on the leaves. The results showed that an increase in air temperature during seed filling period (R5-R7) in the early planting dates is the most important factor in reducing seed quality. Also, a significant negative correlation was observed between seed infection by Phomopsis sp. and Nigrospora sp. and seed germination and vigor. Delayed planting by reducing the temperature during seed filling period and seed infection by this pathogenic fungi, increased seed quality. Foliar application of benomyl fungicide and potassium silicate by reducing seed infection by pathogenic fungi, improved the quality of soybean seeds. Thus, the delay in planting of precocious cv. Williams, foliar application of potassium silicate and benomyl fungicide in reproductive stages can be advised as the strategies to enhance the seed quality of this cultivar to the farmers and seed producers in Gorgan.



    1. 1.       قادری­فر ف و سلطانی ا (1389) کنترل و گواهی بذر. انتشارات جهاد دانشگاهی مشهد. 200 ص.
    2. Aston MJ and Jones MM (1976) A study of the transpiration surfaces of Avena sterilis L. var. algerian leaves using monosilicic acid as a tracer for water movement. Planta. 130 (2): 121-129.
    3. Balducchi AJ and McGee DC (1987) Environmental factors influencing infection of Soybean seeds by Phomopsis and Diaporthe species during seed maturation. Plant Disease. 71(3): 209-212.
    4. Barker AV and Pilbeam DJ (2007) Handbook of plant nutrition. USA. 613 p.
    5. Basra AS (2002) Seed quality (basic mechanisms and agricultural implications). Darya Ganj, New Delhi. 389 p.
    6. Bossi Buck G, Korndorfer GH, Nolla A and Coelho L (2008) Potassium silicate as foliar spray and rice blast control. Journal of Plant Nutrition. 31(2): 231-237.
    7. Calvino PA, Sadras VO and Andrade FH (2003) Quantification of environmental and management effects on the yield of late-sown soybean. Field Crops Research. 83(1): 67-77.
    8. Dornbos DL and Mullen RE (1991) Influence of stress during soybean seed fill on seed weight, germination and seedling growth rate. Canadian Jourrnal of Plant Science. 35 (2): 373-383.
    9. Egli DB, Tekrony DM, Heitholt JJ and Rupe J (2005) Air temperature during seed filling and soybean seed germination and vigor. Crop Science. 45(4): 1329-1335.
    10. Fehr WR and Caviness CE (1980) Stages of soybean development. Iowa Agriculture. Experiment. Stn.
    11. Frankaneto JB, Krzyanowski FC, Henning AA, West SH and Miranda LC (1993) Soybean seed quality as affected by shriveling due to heat and drought stresses during seed filling. Seed Science and Technology. 21(1): 107-116.
    12. Gibson LR and Mullen RE (1996) Soybean seed quality reductions by high day and night temperature. Crop Science. 36(6): 1615-1619.
    13. Hampton JG and TeKrony DM (1995) Handbook of vigor test methods. The International Seed Testing Association, Zurich. 117 p.
    14. Hoffman DD, Hartman GL, Mueller DS, Leitz RA, Nickell CD and Pedersen WL (1998) Yield and seed quality of soybean cultivars infected with Sclerotinia sclerotiorum. Plant Disease. 82(7): 826-829.
    15. Jardine DJ (1991) The Lowa soybean pod test for predicting Phomopsis seed decay in Kansas. Plant Disease. 75(5): 523-525.
    16. Jordan EG, Manandhar JB, Thapliyal PN and Sinclair JB (1988) Soybean seed quality of 16 cultivars and four maturity groups in Illinois. Plant Disease. 72(1): 64-67.
    17. Keigley PJ and Mullen RE (1986) Changes in soybean seed quality from high temperature during seed fill and maturation. Crop Science. 26(6): 1212-1216.
    18. Kunwar IK, Manandhar JB and Sinclair JB (1986) Histopathology of soybean seeds infected with Alternaria alternate. Phytopathology. 76(5): 543-546.
    19. Li S, Smith JR and Nelson RL (2011) Resistance to Phomopsis seed decay identified in maturity group V soybean plant introductions. Crop Science. 51(6): 2681-2688.
    20. Liang YC, Sun WC, Si J and Romheld V (2005) Effects of foliar- and root-applied silicon on the enhancement of induced resistance to powdery mildew in cucumis sativus. Plant Pathology. 54(5): 678-685.
    21. Ma JF, Miyake Y and Takahashi E (2001) Silicon as a beneficial element for crop plants. In: Datnoff, L.E., Snyder, G.H., and Korndorfer, G.H. Silicon in agriculture. Amesterdam, Holand. Elsevier. Pp. 17-39.
    22. Mayhew WL and Caviness CE (1994) Seed quality and yield of short-season soybean genotypes. Agronomy. 86(1): 16-19.
    23. Mengistu A and Heatherly LG (2006) Planting date, irrigation, maturity group, year, and environment effects on Phomopsis longicolla, seed germination, and seed health rating of soybean in the early soybean production system of the midsouthern USA. Crop Protection. 25(2): 310-317.
    24. Mengistu A, Castlebury L, Smith R, Ray J and Bellaloui N (2009) Seasonal progress of Phomopsis longicolla infection on soybean plant parts and its relationship to seed quality. Plant Disease. 93(10): 1009-1018.
    25. Menzies J, Bowen P, Ehert D and Glass ADM (1992) Foliar applications of potassium silicate reduce severity of powdery mildew on cucumber, muskmelon, and zucchini squash. Journal of American Society of Horticulture Science. 117(6): 902-905.
    26. Osorio JA and McGee DC (1992) Effect of freeze damage on soybean seed mycoflora and germination. Plant Disease. 76(9): 879-882.
    27. Ranganathan S, Suvarchala V, Rajesh YBRD, Srinivasa Prasad M, Padmakumari AP and Voleti SR (2006) Effects of silicon sources on its deposition, chlorophyll content, and disease and pest resistance in rice. Biological Plantarum. 50(4): 713-716.
    28. Rodrigues FA, Duarte HSS, Domiciano GP, Souza CA, Korndorfer GH and Zambolim L (2009) Foliar applications of potassium silicate reduce the intensity of soybean rust. Australasian Plant Pathology. 38(4): 366-372.
    29. Rodrigues FA, Duarte HSS, Rezende DC, Wordellfilho JA, Korndorfer GH and Zambolim L (2010) Foliar spray potassium silicate on the control of angular leaf spot on beans. Journal of Plant Nutrition. 33(14): 2082-2093.
    30. Roy KW, Hershman DE, Rupe JC and Abney TS (1997) Sudden death syndrome of soybean. Plant Disease. 81(10): 1100-1111.
    31. Samarah NH and Abu-YahyaA (2008) Effect of maturity stages of winter and spring sowen chickpea (Cicer arientinum L.) on germination and vigor of the harvested seeds. Seed Science and Technology. 36(1): 177-190.
    32. Sastawa BM, Lawan M and Maina YT (2004) Management of insect pests of soybean: effects of sowing date and intercropping on damage and grain yield in the Nigerian Sudan savanna. Crop Protection. 23(2): 155-161.
    33. Sinclair JB and Backman PA (1989) Compendium of soybean diseases. Published by The American Phytopathological Society. 106 p.
    34. Soto-Arias JP and Munkvold GP (2011) Impacts of foliar fungicides on infection of soybean by Phomopsis spp. in Iowa, USA. Crop Protection. 30(5): 577-580.
    35. Spears JF, TeKrony DM and Egli DB (1997) Temperature during seed filling and soybean seed germination and vigour. Seed Science and Technology. 25(2): 233-244.
    36. Spilker DA, Schmitthenner AF and Ellett CW (1981) Effects of humidity, temperature, fertility, and cultivar on the reduction of soybean seed quality by phomopsis sp. Phytopathology. 71(10): 1027-1029.
    37. Tekrony DM, Egli DB, Stuckey RE and Balles J (1983) Relationship between weather and soybean seed infection by Phomopsis sp. Phytopathology. 73(6): 914-918.
    38. Tekrony DM and Egli DB (1985) Effect of Benomyl application on soybean seedborne fungi, seed germination, and yield. Plant Disease. 69(9): 763-765.
    39. Wrather JA, Sleper DA, Stevens WE, Shannon JG and Wilson RF (2003) Planting date and cultivar effects on soybean yield, seed quality, and Phomopsis sp. seed infection. Plant Disease. 78(5): 529-532.
    40. Wrather JA, Shannon JG, Stevens WE, Sleper DA and Arelli AP (2004) Soybean cultivar and foliar fungicide effects on Phomopsis sp. seed infection. Plant Disease. 88(7):721-723.
    41. Zanakis GN, Ellis RH and Summerfield RJ (1994) A comparison of changes in vigour among three genotypes of soybean (Glycine max) during seed development and maturation in three temperature regimes. Experimental Agriculture. 30(1): 157-170.
    42. Zhang AW, Hartman GL, Curio-Penny B, Pedersen WL and Becker KB (1999) Molecular detection of Diaporthe phaseolorum and Phomopsis longicolla from soybean seeds. Phytopathology. 89(9): 796-804.