Document Type : Research Paper


1 M.Sc. graduate student, Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

2 Assistant Professor, Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

3 Associate Professor, Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

4 Assistant Professor, Department of Water Engineering, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran


Drought preconditioning (DP) is a method of irrigation with low frequency and longer duration, to extend the root system and suppress drought damage in plants. In the present study, the effect of DP for 30 days on increasing tolerance to drought stress in two turfgrass species, creeping bentgrass (Agrostis stolonifera cv.Palustris) and tall fescue (Festuca arundinacea cv. Greystone) was investigated. DP and control plants were irrigated for 50 days with different levels of soil suction (20-23,40-43, 50-53 and 70-73 centibar), and finally, plants were watered up to field capacity point for 15 days, to recover from stress. Results showed that DP significantly lead to reduction in shoot length and increase in root length in both turfgrass species. DP plants showed less shoot dry and fresh weight and also less electrolyte leakage (EL) at each levels of stress, in compare with the control. Furthermore, DP plants showed greater reduction in EL after recovery. DP treatment also reduced leaf RWC at the end of 50 days of stress and after recovery in tall fescue, but in creeping bentgrass retained it at the control level. Overall, it appears that DP treatment can increase tolerance to drought stress in two species with increasing root length, reducing shoot length and EL and preserving leaf RWC.


1 . تهرانی­فر ع، سلاح­ورزی ی، گزانچیان ع و آرویی ح (1388) بررسی پاسخ­های گراس­های بومی و وارداتی در چگونگی اجتناب از خشکی (2- بخش هوایی). علوم باغبانی (علوم و صنایع کشاورزی). 23 (1): 1-9.
2. دولت آبادیان ا، مدرس ثانوی س ع م  و شریفی م (1388) اثر تنش کم‌آبی و محلول‌پاشی اسید آسکوربیک بر میزان فعالیت آنزیم­های آنتی­اکسیدان و برخی تغییرات بیوشیمیایی در برگ ذرت دانه‌ای (Zea maize L.). زیست‌شناسی ایران. 22(3): 407-422.
3. سلاح‌ورزی ی، تهرانی‌فر ع، گزانچیان ع و آرویی ح (1387). بررسی پاسخ‌های گراس‌های بومی و وارداتی در چگونگی اجتناب از خشکی. علوم باغبانی (علوم و صنایع کشاورزی). 2(22): 1-12.
4. علیزاده ا (1383) رابطة آب و خاک و گیاه. انتشارات دانشگاه امام رضا. مشهد.
5. کافی م و مهدوی دامغانی ع (1381) مکانیسم‌های مقاومت گیاهان به تنش‌های محیطی (ترجمه). انتشارات دانشگاه فردوسی مشهد، 467 ص.
6. Amiard V, Bertrand AM, Billard JP, Huault C, Keller F and Prudhomme MP (2003) Fructans, but not the sucrosyl-galactosides, raffinose and loliose, are affected by drought stress in perennial ryegrass. Journal of Plant Physiology. 132: 2218-2229.
7. Efeoglu B, Ekmekci Y and Cicek N (2009) Physiological responses of three maize cultivars to drought stress and recovery. South African Journal of Botany. 75: 34-42.
8. Fry J and Haung B (2004) Applied Turfgrass Science and Physiology. John Wiley and Sons Inc, Hoboken, NJ.
9. Fu J and Huang B (2001) Involvement of antioxidants and lipid peroxidation in the adaptation of two cool- season grasses to localized drought stress. Environmental and Expremental Botany. 45: 105-114.
10. Gazanchian A, Hajheidari M, Khosh Kholgh Sima NA and Salkadeh GH (2007) Proteom response of Elymus elongatum to sever water stress and recovery. Experimental Botany. 58: 291-300.
11. Hoffman L, DaCosta M, Scott Ebdon J and Zhao J (2012) Effects of drought preconditioning on freezing tolerance of perennial ryegrass. Environmental and Experimental Botany. 79: 11-20.
12. Inze D and Montagu M Van (1995) Oxidative stress in plants. Current Opinion in Biotechnology. 6: 153-15.
13. Jiang Y and Huang B (2001) Osmotic adjustment and root growth associated with drought preconditioning enhanced heat tolerance in Kentucky bluegrass. Crop Science. 41: 1168-1173.
14. Jinrong L, Xiaorong X, Jianxiong D, Jixiong S and Xiaomin B (2008) Effects of simultaneous drought and heat stress on Kentucky bluegrass. Scientia Horticulturae. 115: 190-195.
15. Jordan J, White R, Vietor D, Hale T, Thomas J and Engelke M (2003) Effect of irrigation frequency on turf quality, shoot density and root length density of five bentgrass cultivars. Crop Science. 43: 282-287.
16. Latimer JG (1992) Drought, paclobutrazol, abscisic acid, and gibberellic acid as alternatives to daminozide in tomato transplant production. Horticulture Science. 117: 243-247.
17. Liu J, Xie X, Du 1, Sun J and Bai X (2008) Effects of simultaneous drought and heat stress on Kentucky bluegrass. Scientia Horticultrae. 115: 190-195.
18. Mahajan S and Tuteja N (2005) Cold, salinity and drought stresses: An overview. Archives of biochemistry and biophysics. 444: 139-158.
19. Peng Y, Chenping X.U, Lixin X.U and Hung B (2011) Improved heat tolerance through drought preconditioning associated with changes in lipid composition, antioxidant enzymes, and protein expression in kentucky bluegrass. Crop science. 2: 807-817.
20. Pound WE and Street JR (2001) Managing turfgrass under drought conditions. Horticulture and Crop Science. 61: 4028-4029.
21. Richardson MD, Karcher DE, Hignight K and Rush D (2008) Drought tolerance and rooting capacity of Kentucky bluegrass cultivars. Crop Science. 48: 2429-2436.
22. Shaoyun L, Su W, Li H and Guo H (2009) Abscisic acid improves drought tolerance of triploid bermudagrass and involves H2O2- and NO-induced antioxidant enzyme activities. Plant Physiology and Biochemistry. 47: 132-138.
23. Trenholm LE (2000) Improving drought tolerance in your Florida lawn. Institute of Food and Agricultural Sciences. ENH57.
24. Turkan I, Bor M, Ozdemir F and Koca H (2005) Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris 1. subjected to polyethylene glycol mediated water stress. Plant Science. 168: 223-231.
25. Watson J, Zhang H and Alien RD (2003) Overexpression of an Arabidopsis peroxisomal ascorbate peroxidase gene in tobacco increases protection against oxidative stress. Plant Cell Physiology. 40: 725-732.
26. Zhou Li, Peng Y and Ma  X (2013) Different response on drought tolerance and post-drought recovery between the small-leafed and the large-leafed white clover (Trifolium repens L.) associated with antioxidative enzyme protection and lignin metabolism. Acta Physiologia Plantarum. 35: 213-222.
27. Zhu J-K (2003) Regulation of ion homeostasis under salt stress. Current Opinion in Biotechnology. 6: 441-445.