Document Type : Research Paper

Authors

1 Research Institute of Forests and Rangelands

2 Biotechnology Department, Research Institute of Forests and Rangelands, Tehran, Ira

3 Faculty of Agriculture, Islamic Azad University, Karaj, Iran

4 Medicinal Platns Division, Research Institute of Forests and Rangelands, Tehran, Iran

5 Medicinal Plants Research Division, Research Institute of Forests and Rangelands, Tehran, Iran

Abstract

Satureja sahendica Bornm. is an endemic medicinal plant which grows wild in natural habitats of west and north-west regions of Iran. In this study, for domestication of Satureja sahendica and study it's essential oil content and composition, the seeds of seven populations were collected from natural habitats and cultivated in three replications as a complete randomized blocks design. The aerial parts of each accession were collected in full flowering stage at three consecutive years. The essential oils were obtained by hydro-distillation and analyzed by GC and GC/MS. The results showed the highest oil yields were obtained at the first year after cultivation. The major compounds in all oils were Thymol, P-cymene and -terpinene with different percentages. The highest amount of thymol was found in the oils of the plants at second year. In the other words, the oils had better quality, in the second year. The percentage of -terpinene were reduced in the oils in the second year and then increased in the third year. Amount of p-cymene did not show similar changes during three studied years. Generaly, it can be said annual plants had higher oil yields and two-year old plants had better oil quality.

Keywords

1 . جمزاد ز (1388) آویشن­ها و مرزهای ایران، مؤسسۀ تحقیقات جنگل­ها و مراتع کشور، تهران، 123 ص.
2 . زرگری ع (1361) گیاهان دارویی، جلد دوم، چاپ سوم، انتشارات دانشگاه تهران، 1001 ص.
3 . عباسی خ، سفیدکن ف و یمینی ی (1384) مقایسۀ بازده و ترکیب­های اسانس دو گونه مرزۀ  S.khuzistanicaو
 S. hortensisبا استفاده از روش تقطیر و استخراج با سیال فوق­بحرانی. تحقیقات گیاهان دارویی و معطر ایران. 21(3): 318-307.
4 . طباطبایی رئیسی ع، خلیقی ا، کاشی ع، اثنی­عشری س، بامداد مقدم ص و دل آذر ع (1386) فعالیت آنتی­اکسیدانی ترکیب‌های شیمی اسانس بخش­های Satureja sahendica Bornm. علوم دارویی. 1: 3-6.
5 . میرحیدر ح (1372) معارف گیاهی، جلد اول، دفتر نشر فرهنگ اسلامی، تهران. 539 ص.
6 . میرزا م، سفیدکن ف و احمدی ل (1375) اسانس­های طبیعی، استخراج، شناسایی کمی و کیفی و کاربرد، انتشارات مؤسسۀ تحقیقات جنگل­ها و مراتع کشور. تهران، 205 ص.
7 . Adams RP (1995) Identification of Essential Oil Components by Gas Chromatography/Mass Spectroscopy. Allured, Coral Stream, IL.
8 . Ahmadi Sh, Sefidkon F, Babakhanlo P, Asgari F, Khademi K and KArimifar MA (2009) Comparing Essential oil composition of Satureja bachtiarica Bunge before and full flowering stages in filed and provenance. Iranian Journal of Medicinal and Aromatic Plants. 25(2): 159-169.
9 . Baher Z, Mirza M and Ghorbanli M (2002) The influence of water stress on plant height, herbal and essential oil yield and composition in Satureja hortensis L. Flavour and Fragrance. 17(14): 275-277.
10 . Baser KHC, Ozek T, Kirimer R and Tumen G (2004) A comparative study of the essential oils of wild and cultivated Satureja hortensis L. Essential Oil Research. 16(5): 422-424.
11 . Ćavar S, Maksimović M, Edita Šolić M, Jerković-Mujkić A and Bešta R (2008) Chemical composition and antioxidant and antimicrobial activity of two Satureja essential oils. Food Chemistry. 111: 648-653.   
12 . Chalchat JC, Gorunovic MS and Maksimovic ZA (1999) Essential oil of Satureja kitaibelii Wierzb. F. aristata (Vand) Hayek, lamiaceae from eastern Serbia. Essential Oil Research. 11: 691-692.
13 . Hajhashemi V, Ghannadi A and Pezeshkian SK (2002) Antinocicieptive and anti-inflammutory effect of Satureja hortensis lL, extracts and essential oil. Ethnopharmacol. 82(2-3): 83-87.
14 . Javidnia K, Miri R, Edraki N and Nasiri A (2005) Chemical constituensts of the volatile oil of Satureja macrantha from Iran. First Seminar of Medicinal and Natural Products Chemistry, Shiraz, Iran, 10-11 May. 86p.
15 . Kurcuoglu M, Then G and Baser KHC (2001) Essential oil constituents of Satureja biossieri from Turkey. Khimiya Prirodnykh Soedinenii. 37(4): 280-281.
16 . Leak G, Gasper F and Santos R (2003) Effect of water on the solubility of essential oils in dense CO2. Essential Oil Research. 15: 172-177.
17 . Novak J, Bahoo L, Mitteregger U and Franz C (2006) Composition of individual essential oil glands of savory (Satureja hortensis L., (Lamiaceae) from Syria. Flavour and Fragrance. 21: 731-734.
18 . Omidbaigi R (1997) Approaches to Production of Medicinal Plants. Tehran, 195 p (In Persian).
19 . Rechinger KH (1982) Satureja in Flora Iranica, Akademische Druck-u. Verlagsamstalt, Graz. 37(4): 495-504.
20 . Rojas LB and Usubillaga A (2000) Composition of the essential oil of Satureja brownei (SW.) Briq. From Venezuela. Flavour and Fragrance. 15: 21-22.
21 . Sefidkon F, Abbasi K and Bakhshi Khaniki G (2006) Influence of drying and extraction methods on yield and chemical composition of the essential oil of Satureja hortensis. Food Chemistry. 99: 19-23.
22 . Sefidkon F and Ahmadi SH (2000) Essential oil of Satureja khhuzistanica Jamzad. Essential oil Research. 12: 427-428.
23 . Sefidkon F, Akbarinia A and Barazandeh MM (2009) Essential oil content and composition of Satureja sahendica Bornm. in different stage of plant growth. Essential Oil Research. 21: 112-114.
24 . Sefidkon F and Jamzad Z (2000) Essential oil of Satureja bachtiarica Bunge. Essential Oil Research. 12: 545-546.
25 . Sefidkon F and Jamzad Z (2004) Chemical variation in the essential oil of Satureja sahendica from Iran. Food Chemistry. 88: 325-328.
26 . Sefidkon F and Jamzad Z (2005) Chemical composition of the essential oil of three Iranian Satureja species (S. mutica, S. macrantha and S. intermedia). Food Chemistry. 91: 1-4.
27 . Sefidkon F, Janzad Z and Mirza M (2004) Chemical variation in the essential oil of Satureja sahendica from Iran. Food Chemistry. 88: 325-328.
28 . Shibamoto T (1987) Retention indices in essential oil analysis, P. 259-275, In: capillary Gas chromatography in Essential oil Analysis. Edits, P. Sandra and C. Bicchi, Dr. Alfred Heuthig Verlag, NewYork.
29 . Simon JE, Chadwick AF and Graker LE (1981) Herbs and Indexed Biblogbraphy. Archon books, 710 p.
30 . Skocibusic M and Bezic N (2004) Phytochemiccal analysis and in vitro antimicrobial activity of two Satureja species essential oil. Phytotherapy Research. 18(12): 964-970.
31 . Tabatabaei Rasti AR, Khalighi A, Kashi A, Asnaashari S, Bamdad Moghadam S and Delazar A (2007) Antioxidant activity and chemical composition of essential oil of  Satureja sahendica Born. Pharmaceutical Sciences. 3: 1-6.
32 . Teimouri M, Bahar Z and Mirza MA (2003) Antimicrobial activity of essential oil of Satereja laxiflora G. Koch before and after flowering. Iranian Journal of Medicinal and Aromatic Plants. 13: 49-68.

33 . Thume G, Baser KHC, Demirci B and Ermin N (1998) The essential oils of Satureja coerulea Janka and Thymus aznavourii Velen. Flavour and Fragrance. 13(1): 65-67.