Document Type : Research Paper
Authors
1 Instructor, Department of Horticulture, College of Aboreihan, University of Tehran, Pakdasht – Iran
2 Professor, College of Agriculture and Natural Resources, University of Tehran, Karaj – Iran.
3 Instructor, Department of Biology, College of Science, University of Tehran, Tehran – Iran
4 Associate Professor, Research Institute of Forests and Rangelands, Karaj – Iran.
5 Assistant Professor, College of Agriculture and Natural Resources, University of Tehran, Tehran – Iran.
Abstract
Five different media with two concentrations and kinds of carbohydrates and peptone treatments were examined for their effectiveness in promoting asymbiotic seed germination of Epipactis veratrifolia. Results revealed significant differences between media on seed germination percentage and protocorm growth and Fast medium has the best effect on both. Carbohydrate treatments had significant effect on seed germination percentage but not on protocorm growth. Modified Fast medium with 5gr l-1 fructose and 12 gr l-1 sucrose and 2 gr l-1 peptone (MFH2P2) was the best medium for seed germination (49.6%) and Modified Fast medium with 30 gr l-1 sucrose and 2 gr l-1 peptone (MFH1P2) was the best medium for protocorm growth (17.3 mm). Media, carbohydrate and peptone influence seed germination percentage, while media and peptone alone influence protocorm growth. In addition, use of in vitro culture significantly reduced relatively long period of time which is necessary for seed germination and protocorm plantlets growth in natural conditions (approximately 2 years).
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