In order to determine the effects of applied phosphate bio-fertilizer on yield and morphological traits of wheat, a research was conducted in Sari Agricultural Sciences and Natural Resources University during 2008-2010. A factorial experiment was performed based on randomized complete block design with four replications. The first factor consists of two levels of phosphate bio-fertilizer (0 and 100 g ha-1) and the second factor was three levels of phosphate fertilizer (0, 60 and 90 kg ha-1). Results showed that bio-fertilizer increased significantly grain yield, spike/m2, seed /spike, spike length, 1000 seeds weight and harvest index, however, had no significant effect on plant height. There was no significant difference between 60 and 90 kg ha-1 phosphate fertilizer treatments obtained with 100 g ha-1 bio-fertilizer (528.40 and 532.95 kg ha-1 respectively) in term of total grain yield. Therefore, application of 60 kg ha-1 phosphate fertilizer with bio-fertilizer instead of conventionally using phosphate fertilizer in Mazandaran (90 kg ha-1), not only save nearly 30 kg ha-1 phosphate fertilizer, but also prevent soil and water pollutions.