Journal of Crops Improvement

Current Issue

By Issue

By Author

By Subject

Author Index

Keyword Index

About Journal

Aims and Scope

Editorial Board

Publication Ethics

Indexing and Abstracting

Related Links

FAQ

Peer Review Process

Journal Metrics

News

Reviewers

Publons Membership and Review Guide

Instructions for removing the reviewers's name from the comments in Word

Impact of Plant Growth-Promoting Rhizobacteria on Biochemical and Yield Traits of Two Durum Wheat Cultivars Under Rainfed and Supplementary Irrigation Conditions

Document Type : Research Paper

Authors

1 Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan,, Iran

2 Assis. Prof., Dept. of Agronomy, Faculty of Agriculture, Ilam University, Ilam, Iran

3 Assoc. Prof., Dept. of Agronomy, Faculty of Agriculture, Ilam University, Ilam, Iran

10.22059/jci.2024.276687.2175

Abstract

Objective: Given the challenges of water scarcity and the need for sustainable agricultural practices, this study was conducted to evaluate the impact of plant growth-promoting bacteria on the physiological traits of durum wheat at the research farm of Ilam University during the 2016-2017 growing season. The experiment was carried out as a factorial based on a randomized complete block design with three replications.
Methods: The factors comprised three levels of plant growth-promoting rhizobacteria (Azospirillum brasilense, Azotobacter chroococcum and non-inoculated), irrigation in two levels (supplementary irrigation and rainfed), and two durum wheat cultivars (Saji and Dehdasht).
Results: Supplemental irrigation significantly increased chlorophyll b and total chlorophyll content by 13.47% and 15.45% compared to rainfed conditions, respectively. Azospirillum and Azotobacter increased carotenoid concentration by 27% and 18.66% compared to the control, respectively. Moreover, the carotenoid concentration in the Saji cultivar was 21.42% higher than in the Dehdasht cultivar. The lowest anthocyanin concentration was observed in both Saji and Dehdasht cultivars under irrigated conditions (0.0055 and 0.0042 mmol g-1 fresh weight, respectively) and without bacterial inoculation. The Dehdasht cultivar had the highest leaf area index (6) compared to the Saji cultivar. A 16.60% decrease in the relative water content of leaves was observed at the reproductive stage under rainfed conditions. Among the two cultivars, the Dehdasht cultivar had a higher relative water content (55.03%). The highest ion leakage was observed under rainfed conditions (85.99%). Azospirillum and Azotobacter bacteria reduced ion leakage by 19.47% and 17.80% compared to the control, respectively. Catalase activity increased under rainfed conditions. Catalase activity was 15% higher in the Dehdasht cultivar than in the Saji cultivar. Plant growth promoting bacteria increased leaf area index, total chlorophyll, chlorophyll a, chlorophyll b, and catalase activity. Seed inoculation with Azospirillum bacteria increased peroxidase activity, and among the two inoculated cultivars, the Dehdasht cultivar had higher enzyme activity (27.60 μmol g-1 fresh weight of leaf). The highest grain yield (2923 kg ha-1) of durum wheat cultivars was obtained under supplemental irrigation conditions and inoculation with plant growth promoting bacteria.
Conclusion: The obtained results demonstrated the positive effect of growth promoting bacteria on common cultivars of the Ilam area under supplementary irrigation

Keywords

References
Abdoli, M., Saeidi, M., Jalali-Honarmand, S., Mansourifar, S., & Eghbal, M. (2013). Evaluation of some physiological and biochemical traits and their relationships with yield and its components in some improved wheat cultivars under post-anthesis water deficit. Journal of Environmental Stresses in Crop Sciences, 6(1), 63-47. (In Persian).
Ahanger, M. A., Qi, M., Huang, Z., Xu, X., Begum, N., Qin, C., & Zhang, L. (2021). Improving growth and photosynthetic performance of drought stressed tomato by application of nano-organic fertilizer involves up-regulation of nitrogen, antioxidant and osmolyte metabolism. Ecotoxicology and Environmental Safety, 216, 112195.‏
Ahmad, Z., Waraich, E. A., Akhtar, S., Anjum, S., Ahmad, T., Mahboob, W., ..., & Rizwan, M. (2018). Physiological responses of wheat to drought stress and its mitigation approaches. Acta Physiologiae Plantarum, 40(4), 1-13.
Ahmadvand, G., & Hajinia, S. (2017). Effect of fungus Piriformospora indica on the grain yield, absorption and radiation use efficiency of millet under different irrigation regimes. Cereal Research, 8(2), 261-272. (In Persian).
Baghkhani, F., & Far ahbakhsh, H. (2009). Effects of drought stress on yield and some physiological characters of three spring Safflower (Carthamus tinctorius) varieties. Journal of Water Research in Agriculture, 3(1), 57-45. (In Persian).
Barnawal, D., Bharti, N., Pandey, S. S., Pandey, A., Chanotiya, C. S., & Kalra, A. (2017). Plant growth-promoting rhizobacteria enhance wheat salt and drought stress tolerance by altering endogenous phytohormone levels and TaCTR1/TaDREB2 expression. Physiologia Plantarum, 161(4), 502-514.
Barrs, H. D., & Weatherly, P. E. (1962). A re-examination of relative turgidity technique for estimating water deficits in leaves. Australian Journal of Biological Science, 15, 413-428.
Basu, M., Bhadoria, P. B. S., & Mahapatra, S. C. (2008). Growth, nitrogen fixation, yield and kernel quality of peanut in response to lim, organic and inorganic fertilizer levels. Bioresource Technology, 99, 4675-4683.
Boroujerdnia, M., Bihamta, M., Alami Said, K., & Abdossi, V. (2016). Effect of drought tension on proline content, soluble carbohydrates, electrolytes and water content of bean. Crop Physiliogy Journal, 8(29), 23-41. (In Persian).
Chakraborty, U., Chakraborty, B. N., & Dey, P. L. (2013). Water stress amelioration and plant growth promotion in wheat plants by osmotic stress tolerant bacteria. World Journal of Microbiology and Biotechnology, 29, 789-803.
Chance, B., & Maehly, A. C. (1995). Assay of catalase and peroxidase. In: Colowick S. P., and N. D. Kaplan. (eds). Methods in Enzymology. Academic Press. New York, 2, 764-791.
Davoodifard, M., Habibi, D., & Davoodifard, F. (2012). Effects of salinity stress on membrane stability, chlorophyll content and yield components of wheat inoculated with plant growth promoting bacteria and humic acid. Iranian Journal of Agronomy and Plant Breeding, 8(2), 71-86. (In Persian).
Esfandiari, E., & Vahdati Rad, A. (2012). Decline of tolerance in leaf photooxidative-stress with age in sunflower. Journal of Plant Biology, 4(14), 1-14. (In Persian).
Esfandiari, E., Shakiba, M. R., Mahboob, S. R., Alyari, H., & Baradaran Firozabadi, M. (2009). Effect of water stress on antioxidant enzymes activities and lipid peroxidation of wheat seedlings. Agriculture science and sustainable production, 19(2), 129-138. (In Persian).
Fahad, S., Bajwa, A. A., Nazir, U., Anjum, S. A., Farooq, A., Zohaib, A., & Huang, J. (2017). Crop production under drought and heat stress: plant responses and management options. Frontiers in Plant Science, 8, 1147.‏
Fanaei, H. R., Azmal, A., & Piri, I. (2017). Effect of biological and chemical fertilizers on oil, seed yield and some agronomic traits of safflower under different irrigation regimes. Journal of Agroecology, 8(4), 551-566. (In Persian).
Ghahremani, M., Ebadi, A. parmoon, G., & Jahanbakhsh, S. (2015). Study of some changes in cellular adaptation of forage sorghum genotypes during stress. Journal Crop Improvement, 17(3), 755-773. (In Persian).
Ghanati, F., Morita, A., & Yokota, H. (2002). Induction of suberin and increase of liginin content by excess Boron in Tabacco cell. Soil Science Plant Nutrition, 48 (3), 357-364.
Ghasemi, H. R., & Mostajerana, A. (2017). Study of associative relationship effects of Azospirillum brasilense (Sp7 and Sp245) on some growth and biochemical indices of wheat seedlings under saline conditions. Journal of Plant Research, 31(3), 629-641. (In Persian).
Gholipour, S., & Ebadie, A. (2018). Study change compatibility metabolites and antioxidant enzyme activities of wheat cultivars under water stress. Journal of Plant Process and Function, 6(19), 219-232
Ghosh, D., Lin, Q., Xu, J., & Hellmann, H. A. (2017). How plants deal with stress: exploration through proteome investigation. Frontiers in Plant Science, 8(1176), 1-2.
Harba, A., Awada, D., & Samarahb, N. (2016). Gene expression and activity of antioxidant phenols, peroxide activity and chlorophyll content in wheat. Acta Physiologiae Plantarum, 16(3), 185-197.
Heidari, G. R., Hasanzadeh, B., Siosemardeh, A., Sohrabi, Y., & Emam, Y. (2015). The effects of drought stress, sulfur and applications on some physiological traits in sunflower. Iranian Journal of Dry Land, 4(1), 23-44. (In Persian).
Hosseini, H. S., Nezami, A., Parsa, M., & Hajmohammadnia Ghalibaf, K. (2011). Effects of Supplementary Irrigation on Yield and Yield Components of Lentil Cultivars in Mashhad. Journal of Water and Soil, 25(3), 625-633. (In Persian).
Inanloofar, M., Omidi, H., & Pazoki, A. (2013). Morphological, agronomical changes and oil content in purslane under drought stress and biological/ chemical fertilizer of nitrogen. Journal of Medicinal Plants, 4(48), 170-184. (In Persian).
IPO International Pasta Organization. (2013). The World Pasta Industry Status Report. www.internationalpasta.org. 44.
Jafarzadeh, L., Omidi, H., & Bostani, A. A. (2014). The study of drought stress and Bio fertilizer of nitrogen on some biochemical traits of Marigold medicinal plant. Journal of Plant Research, 27(2), 180-193. (In Persian).
Jha, P. K., Kumar, S. N., & Ines, A. V. (2018). Responses of soybean to water stress and supplemental irrigation in upper Indo-Gangetic plain: Field experiment and modeling approach. Field Crops Research, 219, 76-86.
Lichtenthaler, H. K., & Wellburn, A. R. (1983). Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Soceity Transactions, 11, 591-592.
Lutts, S., Kinet, J. M., & Bouharmont, J. (1996). NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Journal of the Annals of Botany, 78, 389-398.
Madeh Khaksar, A., Naderi, A., Ayeneh Band, A., & Lack, S. (2014). Simultaneous effect of irrigation and irrigation-off on physiological traits related with yield of maize. Journal of Crop Production Research, 6(1), 63-78. (In Persian).
Mafakheri, K., Bihamta, M. R., & Abbasi, A. R.  (2016). Assessment of antioxidant enzymes activity and peroxidation of membrane lipid in cowpea (Vigna unguiculata L.) genotypes under normal and drought stress condition. Iranian Journal of Filed Crop Science, 47(2). 217-232. (In Persian).
Mazaheri-Laghab., H., Nouri. F., & Zare Abyaneh. H., (2009). The effects of supplementary irrigation on the yield and yield components of three cultivars of sunflower in dry farming. Agronomy Journal, 83, 72-80. (In Persian).
Meshkat, S. M., Kazemi Tabar, S. K., & Gholamali, R. (2016). Effect of drought stress on morphological characteristics and antioxidants in different tobacco varieties in dryland conditions. Second national Congress on the Development and Promotion of Agricultural Engineering and Soil Science of Iran, Tehran, Association for the Development and Promotion of Basic Sciences and Techniques. (In Persian).
Moosavi, S. G. R., & Ramazani, S. H. R.  (2017). Effect of drought stress on root yield and some morpho-physiological traits in different genotypes of sugar beet. Journal of Crop Science and Biotechnology, 20(3), 167-174.
Moradi, I., & Zarea, M. J. (2021). Grain Response of three Wheat Cultivars to Azospirillum Inoculation in two Different Sowing Dates. Journal of Agricultural Science and Sustainable Production, 31(1), 259-273. (In Persian).
Movludi, A., Ebadi, A., Jahanbakhsh, S., Davari, M., & Parmoon, G. H. (2014). The effect of water deficit and nitrogen on the antioxidant enzymes activity and quantum yield of barley (Hordeum vulgare L.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 42, 398-404.
Nasrolahzadeh, S., Shirkhani, A., Zehtab Salmasi, S., & Choukan, R. (2016). Effects of biofertilizer and chemical fertilizer on mize yield in different irrigation conditions. Applied Field Crops Research, 29(4), 72-86. (In Persian).
Nouriyani, H. (2017). Effect of paclobutrazol on the amount of redistribution of assimilates to the grain of three cultivars of wheat under heat tension conditions. Environmental Stresses in Crop Sciences, 4(9), 407-415. (In Persian).
Parvizi, K.., & Farnia, A., & Hedayati, a. (2019). Evaluation the effect of bio fertilizer nitroxin and superabsorbent on growing traits and yield components of corn in water stress condition. Horticultural Plant Nutrition, 2(1), 99-115. (In Persian).
Pazoki, A. (2016). Effects of humic acid and plant growth promoting rhizobacteria on yield and yield components of durum wheat under drought stress condition in Shahr-e-Rey region. Cereal Research, 6(1), 105-117. (In Persian).
Pourebtehaj, M., Habibi, D., Paknejad, M., Davoodi Fard, M., & Farahani Pad, P. (2012). Effect of plant growth promoting rhizobacteria and foliar application of silicic acid and amino acids on destruction antioxidant enzyme activity of barley under drought stress. Iranian Journal of Agronomy and Plant Breeding, 8(2), 147-160. (In Persian).
Rashidi, S., Ebadie, A., Parmoon, G., & Jahanbakhsh, S. (2015). Effect of various nitrogen sources on physiological and biochemical changes of beans under waterstress conditions. Journal plant process function, 3(9), 97-110. (In Persian).
Razmi, Z., & Hamidi, R. (2016). Effect of different priming treatments and different irrigation intervals on biochemical characteristics of two bread wheat cultivars (Triticum aestivum). Crop Physiology Journal, 29(8), 73-87. (In Persian).
Rezaei Nia, N., Ramroudi, M., Galavi, M., & Forouzandeh, M. (2016). Effects of Bio-fertilizers on Physiological Traits and Absorption of Some Nutrients of Chicory (Cichoriu mintybus L.) in Response to Drought Stress. Iranian Journal of Field Crops Research, 15(4), 925-938. (In Persian).
Sairam, R., & Saxena, D. C. (2000). Oxidative stress and antioxidants in wheat genotypes: possible mechanism of water stress tolerance. Journal Agronomy Crop Science, 114, 55-61.
Saroy Bahari, S. H., Pirdashti, H., & Oghabneshin, Y. (2018). Response of chlorophyll and physiological parameters of basil to plant growth promoting rhizobacteria under salinity stress. Journal Plant Process Function, 6(19), 89-104.
Sharma, A., & Zheng, B. (2019). Melatonin mediated regulation of drought stress: Physiological and molecular aspects. Plants, 8(7), 190.
Tabatabaei, S., & Jahan, M., & Hajmohammadnia Ghalibaf, K. (2020). The Effect of biological and nitrogen chemical fertilizers on yield and yield component of bean (Phaseolus vulgaris L. ) under water stress conditions. Environmental Stresses in Crop Sciences, 13(1), 145-154.
Taherkhanchi, A., Akbari, G. A., Mohammad Modarres-Sanavy, S. A., & Majid Ghorbani, J. (2014). Efect of different irrigation regimes and zeolit super absorbent on the yield and yield components of forage sorghum. Journal of Crop Improvement, 15(3), 153-141. (In Persian).
Tale Ahmad, S., & Haddad, R. (2010). Evaluation of effects of bio-fertilizers on some physiological and biochemical traits in soybean under water deficit condition. Seed and Plant Production, 26(2), 207-225. (In Persian).
Tavakoli, M., & Jalali, A. H. (2016). Effect of different biofertilizers and nitrogen fertilizer levels on yield and yield components of wheat. Journal of Crop Production and Processing, 6(21), 34-45. (In Persian).
Verma, G., Srivastava, D., Tiwari, P., & Chakrabarty, D. (2019). ROS Modulation in Crop Plants under Drought Stress. Reactive Oxygen, Nitrogen and Sulfur Species in Plants: Production, Metabolism, Signaling and Defense Mechanisms, pp. 311-336.
Wanger, G. J. (1979). Content and vacuole extra vacuole distribution of neutral sugars, free amino acids, and anthocyanins in protoplast. Plant Physiology, 64, 88-93.
Wu, S. C., Caob, Z. H., Lib, Z. G., Cheunga, K. C., & Wong, M. H. (2005). Effects of bio fertilizer containing N-fixer, P. and K solubilizes and AM fungi on maize growth: a greenhouse trial. Geoderma, 125, 155-166.
Yousefpoor, Z., Yadavi, A., Balouchi, H., & Farajee, H. (2014). Evaluation of yield and some physiological, morphological and phonological characteristics in sunflower (Helianthus annuus L.) influenced by biological and chemical fertilizer of nitrogen and phosphorus. Journal of Agroecology, 6(3), 508-509. (In Persian).
Journal of Crops Improvement
Volume 26, Issue 4
January 2025
Pages 687-705
Files
Share
How to cite
Statistics