Esmaeil Gholinezhad; Bakhtiar Lalehgani; Shahryar Kazemi
Abstract
In order to investigate the effect of polyamines on antioxidant activity and biochemical traits of Camelina under salinity stress conditions, a pot (as poen air) experiment was conducted at Payame Noor University (PNU), Iran in March 2022 as factorial layout based on a completely randomized design with ...
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In order to investigate the effect of polyamines on antioxidant activity and biochemical traits of Camelina under salinity stress conditions, a pot (as poen air) experiment was conducted at Payame Noor University (PNU), Iran in March 2022 as factorial layout based on a completely randomized design with 12 treatments and 3 repetitions. The salinity treatment was at three salinity levels (0, 15 and 30 dS/m) and 4 foliar spraying levels including 1- spermine (2 mM), 2- spermidine (2 mM), 3- putrescine (2 mM) and control (pure water foliar spraying). The results showed that with the increasing salinity stress, oil and protein contents and dry seed weight, chlorophyll index, shoot organic and mineral percentage decreased, but protein percentage, shoot ash percentage, soluble sugars, antioxidant activity and ion leakage percentage increased significantly. Salinity stress of 30 and 15 ds/m compared to the control, reduced the chlorophyll index (32 and 15 percent) and mineral percentage (19 and 3 percent), oil percentage (17 and 9 percent), dry oil weight in pot (62 and 21 percent) and dry seed weight (52 and 10 percent), but protein percentage (9 and 3 percent), ash percentage (33 and 30 percent), soluble sugars (32 and 20 percent) and antioxidant activity (21 and 13 percent) increased, respectively. Also, foliar spraying with polyamines increased the above traits, but decreased the percentage of ion leakage. Therefore, in order to decreasing the effects of salinity stress and increasing the dry seed and oil weights in pot, spraying with polyamines, especially spermine, is suggested.
Mahtab Noori; Farangis Ghanavati; Gholam Reza Bakhshi khaniki; Hamid Sobhanian; Hamid Reza Fanay
Abstract
Objective: In order to investigate the effect of irrigation intervals, 15 selected Okra genotypes from the National Gene Bank of Iran were cultivated and evaluated in a randomized complete block design with three replications in the 2020-2021 crop year in the experimental research farm of the Seedling ...
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Objective: In order to investigate the effect of irrigation intervals, 15 selected Okra genotypes from the National Gene Bank of Iran were cultivated and evaluated in a randomized complete block design with three replications in the 2020-2021 crop year in the experimental research farm of the Seedling and Seed Breeding Research Institute in Karaj (Seed and Plant Improvement Institute).Methods: After the plants entered the three-leaf stage, irrigation was done once every 5 and 10 days.Results: The results of the analysis of variance showed that the effect of drought stress on all measured traits, including plant height, fruit yield, biological yield, number of ripe fruits, weight of ripe fruit, diameter of ripe fruit, number of seeds per ripe fruit, 1000-seed weight, sugar, and protein content, was statistically significant. Under drought-stress conditions, the mean of all evaluated traits (except soluble sugars) was reduced. The highest fruit yield (12.06 tons per hectare) belonged to genotype 10, which decreased by 37.5% compared to the non-stress conditions of this genotype. Genotypes 1 and 12 had the least change in protein content, genotypes 4 and 6 had the least change in sugar content, and genotypes 9 and 11 had the least change in biological function. Conclusion: Based on the results obtained from this experiment, the best genotype in terms of fruit yield was genotype 10, and in terms of biological function, genotypes 9 and 11 were recommended for use in areas with water shortages.
Hamed Narimani; Raouf Seyed sharif; fatemeh aghaei
Abstract
In order to study the effect of foliar and soil application of Zinc on grain weight and some biochemical traits of wheat (Triticum aestivum L.) under soil salinity, an experiment has been conducted as factorial based on randomized complete block design with three replications in research greenhouse of ...
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In order to study the effect of foliar and soil application of Zinc on grain weight and some biochemical traits of wheat (Triticum aestivum L.) under soil salinity, an experiment has been conducted as factorial based on randomized complete block design with three replications in research greenhouse of University of Mohaghegh Ardabili in 2018-2019. Experimental factors include soil salinity levels [control and salinity of 30, 60, and 90 mM] and four methods of zinc application [no zinc as control, soil application zinc as ZnSO4, foliar application nano zinc oxide, and combination of soil and foliar application of zinc]. Results show that both application of ZnSo4 and foliar application nano Zn oxide under 90 mM soil salinity condition increase the catalase and peroxidase enzymes activity, anthocyanin, proline, and soluble sugars content by 20.24%, 17.68%, 13.16%, 32.88%, and 14.08%, respectively, in comparison with no application of zinc under 90 mM soil salinity condition. Also, both soil application of ZnSo4 and foliar application of nano Zn oxide under non-salinity condition decrease hydrogen peroxide and malondialdehyde content in comparison with no application of Zinc under 90 mM soil salinity. Both soil application ZnSo4 and foliar application nano Zn oxide under non-salinity condition has had the highest grain weight (1.016 g per plant), compared to the application of this treatment combination at other salinity levels. It seems that both application of ZnSo4 and nano Zn oxide can increase weight yield of wheat under salinity condition due to their ability in improving biochemical traits.
Seyedahmad Kalantarahmadi; Jahanfar Daneshian
Abstract
The present experiment has been carried out as a split plot based on randomized complete block design with three replications in Safiabad Agricultural and Natural Resources Research and Education Center of Dezful between 2012 and 2014. The main plots is consisted of 3 sowing dates (November 5, November ...
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The present experiment has been carried out as a split plot based on randomized complete block design with three replications in Safiabad Agricultural and Natural Resources Research and Education Center of Dezful between 2012 and 2014. The main plots is consisted of 3 sowing dates (November 5, November 25, and December 15) with the sub plots including 10 levels of foliar application (100, 200, 300 mg.lit-1 ascorbic acid, 100, 200, 300 μmol ascorbic acid, 10, 20, 30 vol% methanol, and the foliar application of distilled water as control treatment). The effect of sowing date and foliar application as well as their interaction has had significant effect on proline, soluble sugars, number of siliques per plant, and seed yield (P≤0.01). Comparing means of sowing date × foliar application shows that the maximum number of siliques per plant (68.96) has been formed in the first sowing date with the foliar application of ascorbic acid (300 mg.lit-1). There has been a significant difference between different sowing dates: the seed yield has decreased in the second and third sowing date, compared to the first one by 145% and 30%, respectively. However, foliar application of experimental treatments has increased the seed yield in all sowing dates, but it is recommended to use ascorbic acid (300 mg.lit-1) in the first and third sowing dates as well as salicylic acid (200 µmol) and methanol (10%) in the second sowing date.
Mehri Mashayekhi; Fariborz Habibi; Mohamad Amiri
Abstract
Mechanisms of drought stress tolerance of GF677 rootstock, peach and almond hybrid, (Prunus persica × Prunus amygdalus) were studied under in vitro conditions. Plantlets of GF677 rootstock were subcultured into the Murashige and Skoog (MS) solid proliferation medium containing 1 mg/l BA (6-Benzyladenine) ...
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Mechanisms of drought stress tolerance of GF677 rootstock, peach and almond hybrid, (Prunus persica × Prunus amygdalus) were studied under in vitro conditions. Plantlets of GF677 rootstock were subcultured into the Murashige and Skoog (MS) solid proliferation medium containing 1 mg/l BA (6-Benzyladenine) and 0.1 mg/l NAA (naphthaline acetic acid) in four drought stress levels 0 (control), 10, 20 and 30 g/l polyethylene glycol 6000 (corresponding to osmotic potentials 0, -0.2, -0.4 and -0.6 Mpa, respectively). After six weeks results showed that induced drought stress had a significant effect on measured parameters. By increasing drought levels in the culture medium, antioxidant enzymes activity (catalase and peroxidase), total protein content and proline content significantly increased; meanwhile soluble sugars had non-significant increasing in the different levels of drought. According to the results it can be concluded that, the most important mechanisms of drought tolerance of GF677 rootstock under in vitro conditions are the use of antioxidant defense system, increasing protein synthesis (enhancing genes expression) and proline accumulation and osmoregulation by soluble sugars had less important.
Fariborz Habibi; Mohammad Esmaeil Amiri
Abstract
Enzymaticactivity and biochemical responsesof two citrus rootstocks [sour orange (Citrus aurantium L.) and trifoliate orange (Poncirus trifoliata Raf.)] to in vitro salinitystress were studied. Explants of both rootstocks were transferred to Murashige and Skoog (MS) solid proliferation medium containing ...
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Enzymaticactivity and biochemical responsesof two citrus rootstocks [sour orange (Citrus aurantium L.) and trifoliate orange (Poncirus trifoliata Raf.)] to in vitro salinitystress were studied. Explants of both rootstocks were transferred to Murashige and Skoog (MS) solid proliferation medium containing 8.9 µM Benzyladenine (BA) and 0.5 µM Naphthaline Acetic Acid (NAA) supplemented with 0, 50, 100, 150, 200 mM of sodium chloride (NaCl) with six replicates. After six weeks, results showed that, rootstock, salinity levels and their interaction, had a significant effect on all of the measured parameters. Antioxidant enzymes activity such as: catalase, peroxidase, ascorbate peroxidase, proline content, soluble sugars and malondialdehyde (MDA) content increased in both rootstocks by increasing salinity level. The amounts of increasing in sour orange rootstock were greater than trifoliate orange, except for peroxidase enzyme and MDA. Total protein content decreased by increasing salinity level in both rootstocks. Sour orange rootstock has higher total protein content than trifoliate orange. According to the results, sour orange rootstock was more tolerant to salt stress.