Akram Vatankhah; Saeed Reezi; Zahra Izadi; Abdorahman Motamedi; Mahdi Ghasemi Varnamkhasti
Abstract
In this study, the tms2 gene, the cause of crown gall, is detected in two Rosa hybrida L. (“Pearl” and “Angelina”) cultivars using electrochemical methods. In this study, stabilization, and hybridization of single-strand DNA (ssDNA) related to tms2 gene, Agrobacterium tumefaciens ...
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In this study, the tms2 gene, the cause of crown gall, is detected in two Rosa hybrida L. (“Pearl” and “Angelina”) cultivars using electrochemical methods. In this study, stabilization, and hybridization of single-strand DNA (ssDNA) related to tms2 gene, Agrobacterium tumefaciens is investigated by electrochemical impedance spectroscopy. For this purpose, the pencil graphite electrode is modified with nanomaterials including reduced graphene oxide and gold nanoparticles. The biosensor could be used to detect infected samples of “Angelina” and “Pearl” roses. DNA/DNA hybridization is performed by placing an electrode modified with ssDNA at a concentration of two (µmol/L) target DNA. Results show proper stabilization and hybridization of DNA at the electrode surface. The designed biosensor has good selectivity. DNA-based nanosensor has advantages such as low cost, simplicity, and scalability and can be the basis for the development of genomic detection tools.
Akram Vatankhah; Zahra Izadi; Saeed Reezi; Abdorahman Motamedi; Mahdi Ghasemi Varnamkhasti
Abstract
Biotic and abiotic stresses lead to the production of reactive oxygen species (ROS) in plants, damage to the host plant, reduction of its strength, and sometimes plant death. Cut flower rose is one of the most popular ornamental plants, suffering from crown gall caused by Agrobacterium tumefaciens. In ...
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Biotic and abiotic stresses lead to the production of reactive oxygen species (ROS) in plants, damage to the host plant, reduction of its strength, and sometimes plant death. Cut flower rose is one of the most popular ornamental plants, suffering from crown gall caused by Agrobacterium tumefaciens. In order to investigate the activity of some antioxidant enzymes and physiological characteristics in two cultivars of cut roses, A. tumefaciens inoculation was performed as a factorial design in a completely randomized design with three replications at the time of grafting by stenting method in Shahrekord university research greenhouse in September 2020. Treatments include contamination (Agrobacterium suspension inoculation and water inoculation) as the first factor and cultivar treatment (Angelina and Pearl) as the second factor. Three months after grafting, results show that the effect of cultivar/infection interaction has significant effect on ion leakage, proline, and guaiacol peroxidase at the level of one percent probability. The highest levels of soluble sugars, MDA and protein are observed in infected samples, 298.6 (μg/g Fw), 488.6 (µmolg-1 Fw) and 36.7 (mg/g Fw), respectively and the highest RWC and leaf area in healthy samples are 68.5% and 21.5 (cm2). In this study, inoculation of the crown gall lead to active oxygen species and oxidative stress, causing some changes in lipids, soluble sugars, and total protein.
Leila Mohammadi; Saeed Rizi; Abdorrahman Mohammadkhani; Rahim Barzegar
Abstract
To evaluate the effect of slow release fertilizer and humic acid on quality of New Guinea Impatiens (Impatiens hawkeri), an experiment established in complete random design with 15 treatments and three replications. Treatments were slow release fertilizer (12-11-18-2.7MgO-8S) in five levels (0, 1.5, ...
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To evaluate the effect of slow release fertilizer and humic acid on quality of New Guinea Impatiens (Impatiens hawkeri), an experiment established in complete random design with 15 treatments and three replications. Treatments were slow release fertilizer (12-11-18-2.7MgO-8S) in five levels (0, 1.5, 3, 4.5 and 6 kg/m3) and humic acid in three levels (0, 2 and 4 kg/m3) that mixed with medium culture. The substrate medium was included 50 percecnt of peat moss, 40 percent of perlite and 10 percent of rice husk (v/v). Five months after seeding, results showed the most flower number (20 number) in 6 kg/m3 mixed with 2 kg/m3 of humic acid, and the most leaf number (122 number) in 3 kg/m3 of slow release fertilizer treatments. The slow release fertilizer treatment showed a positive significant effect in all traits. Also, humic acid had a positive significant effect on plant height, leaf and shoot number, leaf area, total chlorophyll, shoot fresh weight, flower diameter, flower life and flower number. Considering that, humic acid in 2-4 kg/m3 and slow release fertilizer in 3-4.5 kg/m3 could be recommended for New Guinea Impatiens cultivation.
Maryam Kosravi; Saeed Rizi; Rahim Barzegar; Gholam Reza Rabiei
Abstract
AbstractIn order to evaluate the effect of vermicompost and humic acid on quantitative and qualitative traits of sod turf, a factorial experiment based on completely randomized design with three replications was established in research farm in Shahrekord University. Treatments were vermicompost (zero, ...
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AbstractIn order to evaluate the effect of vermicompost and humic acid on quantitative and qualitative traits of sod turf, a factorial experiment based on completely randomized design with three replications was established in research farm in Shahrekord University. Treatments were vermicompost (zero, 5, 10 and 15 v/v percent) and humic acid (zero, 150 and 250 g/m2), respectively. Some traits such as shoot nitrogen and phosphorus content, shoot height, shoot dry and wet weight, total root length, root fresh and dry weight and root dry weight were evaluated. The results showed that the application of vermicompost and humic acid had a positive significant effect on shoot dry weight, sod root dry weight, total dry weight of roots, fresh weight of root, shoot nitrogen and phosphorous content compared to control. The vermicompost in 15% v/v and humic acid in 150 g/m2 treatments had the most positive effect on measured traits and increased nitrogen (25.4 percent), phosphorous (39.6 percent) content, shoot dry weight (49.7 percent), root weight (83.3 percent), total root dry weight (215 percent) and sod root dry weight (204 percent). In this research application of vermicompost and humic acid increased uptake of nitrogen and phosphorus and it increased the sod quality.
Leila Mohammadi; Saeed Rizi; Rahim Barzegar
Abstract
To evaluate the effect of arbuscular mycorrhiza (Glomus mosseae) under salt stress on physiological and some nutrient absorption in New Guinea Impatiens (Impatiens hawkeri), an experiment established in complete random design with three replications (three pots for each replication) in greenhouse in ...
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To evaluate the effect of arbuscular mycorrhiza (Glomus mosseae) under salt stress on physiological and some nutrient absorption in New Guinea Impatiens (Impatiens hawkeri), an experiment established in complete random design with three replications (three pots for each replication) in greenhouse in Shahrekord University in 2014. Treatments were inoculation of substrate with three levels of arbuscular mycorrhiza (0, 8 and 16 percent, v/v) and sodium chloride (0, 15 and 30 Mm). The substrate medium was included 50 percecnt of peat moss, 40 percent of perlite and 10 percent of rice husk (v/v). The mycorrhizal inoculation done with transplanting and salt stress treatment was applied with irrigation water after stablishment of transplants. Some traits such as nitrogen, phosphorous, potassium, sodium and proline content, dry and fresh root weight and root colonization percentage were evaluated. The results showed that 16 percent of mycorrhiza treatment had significant effect on nitrogen (2.31 percent) and phosphorous (0.339 percent) and it’s interaction with 30 Mm of salt stress had significant effect on proline content (0.754 micromol/gfw) and root colonization (35 percent) percentage. Based on this research the difference between mycorrhiza treated plants and other treatments was significant and it seems that the application of mycorrhiza in medium, can increase salt tolerance in New Guinea Impatiens by effect on some physiological traits.