Atena Tanoori; Azim Ghasemnezhad; Mahdi Alizadeh
Abstract
Modern biotechnology and genetic engineering using techniques and special laboratory tools plays an important role in the pharmaceutical industry and medicinal plant propagation. Among these techniques, it can be noted that a wide range of elicitors are widely used for inducing metabolites ...
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Modern biotechnology and genetic engineering using techniques and special laboratory tools plays an important role in the pharmaceutical industry and medicinal plant propagation. Among these techniques, it can be noted that a wide range of elicitors are widely used for inducing metabolites at invitro culture. Due to the pharmaceutical properties of artichoke and its history, it has been deeply noticed by pharmaceutical industry and its cultivation is expanding rapidly. In present study the effect of two elicitors' methyl jasmonate (MJ) and salicylic acid (SA) on the callus mass growth and survival of invitro culture was investigated. Callus cultures of artichoke were established by transferring seedling on solidified MS medium supplemented with different concentrations of salicylic acid and methyl jasmonate. Four weeks after subculture, the callus was collected and weighed. Results showed that the SA supplementation reduced fresh weight of callus. Contrary to fresh weight, a direct increase was observed in dray weight by increasing the level of SA. Opposite to SA different concentration of MJ not only did not changed the fresh weight, but also reduced the dry weight of callus. Study the pigment changes under different treatments of SA and MJ showed that, contrary to cartonoid a direct reduction was observed in chlorophyll content by increasing in both SA and MJ concentration. Finally it can be suggested that, however both SA and MJ as most important elicitors increase secondary metabolite production of callus, their availability in high concentration affect the cell grow activity, callus survival and the biomass production.