Ebrahim Brouki milan; Leyla Hassni; Babak Abdollahi Mandoulakani; Reza Darvishzadeh; Fatemeh Kheradmand; Abbas Hassani
Abstract
A completely randomized design (CRD) with three replications was conducted at greenhouse of Urmia University in 2013 to study the effect of different methyl jasmonate concentrations (0, 0.1 and 0.5 mM) on the activity of enzymes polyphenol oxidase, phenylalanine ammonia lyase, catalase, ascorbate peroxidase, ...
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A completely randomized design (CRD) with three replications was conducted at greenhouse of Urmia University in 2013 to study the effect of different methyl jasmonate concentrations (0, 0.1 and 0.5 mM) on the activity of enzymes polyphenol oxidase, phenylalanine ammonia lyase, catalase, ascorbate peroxidase, guaiacol peroxidase and total protein content in basil. Activity of the enzymes and total protein content was measured at flowering stage 0, 24, 48 and 72 hours after foliar application of methyl jasmonate. Analysis of data was carried out in a split plot in time design which methyl jasmonate concentrations and sampling times were considered as main and sub-plots, respectively. The results of the investigation showed the maximum activity of phenylalanine ammonia lyase and guaiacol peroxidase at concentrations of 0.5 and 0.1 mM, respectively, 48 and 72 hours after spraying. The effect of methyl jasmonate on the activity of polyphenol oxidase, catalase, ascorbate peroxidase and total protein content was significant (P≤0.01). The most activity of the catalase, ascorbate peroxidase and total protein obtained in a concentration of 0.5 mM methyl jasmonate. Significant differences were observed among sampling times after methyl jasmonate spraying for catalase activity which increased 48 and 72 hours after spraying (P≤0.05). Therefore, methyl jasmonate spraying with a concentration of 0.5 mM could increase the activity of the antioxidant enzymes and total protein in basil.
Alireza Pirzad; Manoochehr Alizade; Abdollah Hassanzadeh Gorttapeh; Reza Darvishzade
Abstract
To evaluate the effect of gamma rays on the seeds and amounts of nitrogen on growth and yield of chamomile (Chamomilla recutita synonym Matricaria chamomilla), a factorial experiment was conducted based on randomized complete block design with three replications at the Research Farm of Agriculture College ...
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To evaluate the effect of gamma rays on the seeds and amounts of nitrogen on growth and yield of chamomile (Chamomilla recutita synonym Matricaria chamomilla), a factorial experiment was conducted based on randomized complete block design with three replications at the Research Farm of Agriculture College of Shahid-Beheshti.-Urmia in 2010. Treatments were gamma rays from cobalt-60 on chamomile seeds cv. ‘Bodegold’ (zero, four, eight, 12, 16, and 20 Gray) and different levels of nitrogen (zero, 50, 100, and 150 kg/ha, urea after appearance of the 4th leaf). The highest leaf dry weight per plant (12.5 g) and per hectare (4194 kg/ha), stem dry weight per plant (49 g) and biomass (19995 kg/ha) were obtained at 8 gray and 100, 100, 150, and 150 kg/ha of nitrogen. The highest dried flower yield at the second harvest (104 kg/ha) were obtained from 20 gray by 100 kg/ha nitrogen, and the yield of the third harvest (122 kg/ha) was from 20 gray of gamma irradiation. While, the highest flower yield at the first harvest (419 kg/ha) and total yield (533 kg/ha) were produced from 0 gray and 150 kg/ha nitrogen. Essential oil percent did not affected by treatments, but despite the significant interaction effect of gamma irradiation and nitrogen on the yield of essential oil, means comparison showed no significant differences among treatments. Yield of dried flower in the first harvest, had the greatest impact on the harvest index.