Madeh Ahmadi; Azim Ghasemnezhad; Mansour Ghorbanpour
Abstract
In order to investigate the effect of some biotic and abiotic elicitors on yield and yield components of stevia plant under different salinity stress, the present study has been perfoemed as a factorial experiment based on the randomized complete design with six replications. It has taken place in a ...
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In order to investigate the effect of some biotic and abiotic elicitors on yield and yield components of stevia plant under different salinity stress, the present study has been perfoemed as a factorial experiment based on the randomized complete design with six replications. It has taken place in a greenhouse under hydroponic and conditions in two consequential experiments. Endophytic fungi isolated from yew (at three levels of control, TB20, TB2-3), foliar treatment with melatonin (at three levels of control, 0.5 μM melatonin tablets, and 0.5 μM Thymus vulgaris extract), and three salinity levels (without salinity, medium salinity 80mM, and high salinity 150mM) have incorporated the present experiments’ treatments. The experimental plants have been investigated based on morphological parameters. In both experiments, results show a positive effect of melatonin and endophytic fungi on characteristics of plant growth’s improvement (one to two times) under salinity stress. Nevertheless, the best growth conditions have been observed in low salinity levels, using thyme extract and TB20 endophytic fungus. Most traits of stevia plant in non-saline conditions along with thyme extract and endophytic fungus TB20 have been at their highest, showing an increase of approximately 1.5 folds, compared to the control. Due to the positive effects of melatonin and endophytes, especially TB20, it is recommended to use melatonin-containing plant extracts as a protective treatment in stevia cultivation in saline conditions.
Atena Tanoori; Azim Ghasemnezhad; Mahdi Alizadeh
Abstract
Modern biotechnology and genetic engineering using techniques and special laboratory tools plays an important role in the pharmaceutical industry and medicinal plant propagation. Among these techniques, it can be noted that a wide range of elicitors are widely used for inducing metabolites ...
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Modern biotechnology and genetic engineering using techniques and special laboratory tools plays an important role in the pharmaceutical industry and medicinal plant propagation. Among these techniques, it can be noted that a wide range of elicitors are widely used for inducing metabolites at invitro culture. Due to the pharmaceutical properties of artichoke and its history, it has been deeply noticed by pharmaceutical industry and its cultivation is expanding rapidly. In present study the effect of two elicitors' methyl jasmonate (MJ) and salicylic acid (SA) on the callus mass growth and survival of invitro culture was investigated. Callus cultures of artichoke were established by transferring seedling on solidified MS medium supplemented with different concentrations of salicylic acid and methyl jasmonate. Four weeks after subculture, the callus was collected and weighed. Results showed that the SA supplementation reduced fresh weight of callus. Contrary to fresh weight, a direct increase was observed in dray weight by increasing the level of SA. Opposite to SA different concentration of MJ not only did not changed the fresh weight, but also reduced the dry weight of callus. Study the pigment changes under different treatments of SA and MJ showed that, contrary to cartonoid a direct reduction was observed in chlorophyll content by increasing in both SA and MJ concentration. Finally it can be suggested that, however both SA and MJ as most important elicitors increase secondary metabolite production of callus, their availability in high concentration affect the cell grow activity, callus survival and the biomass production.