Document Type : Research Paper
Authors
1 Assistant Professor, Department of Horticulture, Faculty of Agriculture, Bu-Alisina Univeristy, Hamedan – Iran
2 M.Sc. Student, Department of Horticulture, Faculty of Agriculture, Bu-Alisina Univeristy, Hamedan – Iran.
Abstract
Frost in early spring is one of the limiting factors in the production of grape. Chemicals offer useful approach to reduce low temperature-induced damages. Different concentrations of salicylic acid including: 0 (control), 0.5, 1 and 2 mM were sprayed twice on 10 year-old ‘Bidaneh Sefid’ grape vines at full bloom stage and changes in leaf soluble carbohydrates, proline and protein levels were measured in the next day morning. Leaf and stem samples were subjected to low temperatures of 0, -2, -4 and -8°C while flower clusters were exposed to temperature of 0, -2 and -4°C for three hours. Salicylic acid at 0.5mM concentration resulted in the highest amounts of soluble carbohydrates while the maximum proline content was observed with 0.5 and 1mM concentrations. Salicylic acid at 2mM concentration caused a decrease in soluble protein level. Salicylic acid, low temperature and their interactions had a significant effect on electrolyte leakage of different tissues. Salicylic acid treatments led to a reduction in electrolyte leakage of stem, leaf and cluster samples, compared to control. It seems that salicylic acid minimizes the negative effects of low temperature with evidence of less membrane damage by up-regulating the accumulation of osmoregulants such as soluble carbohydrates and proline.
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