Introduce the Suitable Medium for Asymbiotic Germination of Epipactis Veratrifolia

Dianati, Shirin; Kafi, Mohsen; Mirmasoumi, Masoud; Mozaffarian, Valiollah; Salami, Ali Reza

doi:10.22059/jci.2013.36107

Introduce the Suitable Medium for Asymbiotic Germination of Epipactis Veratrifolia

Document Type : Research Paper

Authors

Shirin Dianati ¹

Mohsen Kafi ²

Masoud Mirmasoumi ³

Valiollah Mozaffarian ⁴

Ali Reza Salami ⁵

¹ Instructor, Department of Horticulture, College of Aboreihan, University of Tehran, Pakdasht – Iran

² Professor, College of Agriculture and Natural Resources, University of Tehran, Karaj – Iran.

³ Instructor, Department of Biology, College of Science, University of Tehran, Tehran – Iran

⁴ Associate Professor, Research Institute of Forests and Rangelands, Karaj – Iran.

⁵ Assistant Professor, College of Agriculture and Natural Resources, University of Tehran, Tehran – Iran.

10.22059/jci.2013.36107

Abstract

Five different media with two concentrations and kinds of carbohydrates and peptone treatments were examined for their effectiveness in promoting asymbiotic seed germination of Epipactis veratrifolia. Results revealed significant differences between media on seed germination percentage and protocorm growth and Fast medium has the best effect on both. Carbohydrate treatments had significant effect on seed germination percentage but not on protocorm growth. Modified Fast medium with 5gr l^-1 fructose and 12 gr l^-1 sucrose and 2 gr l^-1 peptone (MFH2P2) was the best medium for seed germination (49.6%) and Modified Fast medium with 30 gr l^-1 sucrose and 2 gr l^-1 peptone (MFH1P2) was the best medium for protocorm growth (17.3 mm). Media, carbohydrate and peptone influence seed germination percentage, while media and peptone alone influence protocorm growth. In addition, use of in vitro culture significantly reduced relatively long period of time which is necessary for seed germination and protocorm plantlets growth in natural conditions (approximately 2 years).

Keywords

Carbohydrate

In vitro culture

Native Orchid of Iran

terrestrial orchid

Peptone

Protocorm growth