Hakimeh Oloumi; Ali Zamani; Hosein Mozaffari; Soudabeh Nourzad
Abstract
Objective: The present research was conducted with the aim of evaluating the effect of melatonin treatment on the tolerance of basil plants to the excess of copper and zinc mineral elements.Methods: The effect of melatonin treatment (at two levels of 0 and 100 micromolar) on the tolerance of basil plants ...
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Objective: The present research was conducted with the aim of evaluating the effect of melatonin treatment on the tolerance of basil plants to the excess of copper and zinc mineral elements.Methods: The effect of melatonin treatment (at two levels of 0 and 100 micromolar) on the tolerance of basil plants to the excess of mineral elements copper (50 and 150 μM) and zinc (50 and 100 μM) as factorial layout based on a completely randomized design with 3 replications and at 2017 in greenhouse conditions at Graduate University of Advanced Technology, Kerman Done.Results: The triple effect of melatonin and zinc and copper and the double effect of melatonin and zinc on the relative water content of leaves, total chlorophyll, carotenoid, protein, soluble and reduced sugar, anthocyanin and flavonoid were significant at 1% probability level. Membrane stability indices and relative water content of leaves were improved by applying melatonin in the basil plant separately and together with copper and zinc metals. The amount of reducing sugars, chlorophyll, and carotenoids decreased under the influence of zinc and copper toxicity.Conclusion: melatonin by improving physiological characteristics, especially membrane stability, as well as flavonoid compounds, anthocyanin, and glutathione; It led to the reduction of the negative effects caused by excessive amounts of copper and zinc, especially at a concentration of 50 micromolar of these two metals on growth parameters.
Ebrahim Brouki milan; Leyla Hassni; Babak Abdollahi Mandoulakani; Reza Darvishzadeh; Fatemeh Kheradmand; Abbas Hassani
Abstract
A completely randomized design (CRD) with three replications was conducted at greenhouse of Urmia University in 2013 to study the effect of different methyl jasmonate concentrations (0, 0.1 and 0.5 mM) on the activity of enzymes polyphenol oxidase, phenylalanine ammonia lyase, catalase, ascorbate peroxidase, ...
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A completely randomized design (CRD) with three replications was conducted at greenhouse of Urmia University in 2013 to study the effect of different methyl jasmonate concentrations (0, 0.1 and 0.5 mM) on the activity of enzymes polyphenol oxidase, phenylalanine ammonia lyase, catalase, ascorbate peroxidase, guaiacol peroxidase and total protein content in basil. Activity of the enzymes and total protein content was measured at flowering stage 0, 24, 48 and 72 hours after foliar application of methyl jasmonate. Analysis of data was carried out in a split plot in time design which methyl jasmonate concentrations and sampling times were considered as main and sub-plots, respectively. The results of the investigation showed the maximum activity of phenylalanine ammonia lyase and guaiacol peroxidase at concentrations of 0.5 and 0.1 mM, respectively, 48 and 72 hours after spraying. The effect of methyl jasmonate on the activity of polyphenol oxidase, catalase, ascorbate peroxidase and total protein content was significant (P≤0.01). The most activity of the catalase, ascorbate peroxidase and total protein obtained in a concentration of 0.5 mM methyl jasmonate. Significant differences were observed among sampling times after methyl jasmonate spraying for catalase activity which increased 48 and 72 hours after spraying (P≤0.05). Therefore, methyl jasmonate spraying with a concentration of 0.5 mM could increase the activity of the antioxidant enzymes and total protein in basil.