Gholamhasan Movahed; Nهئش Ahmadi; Ahmad Moieni; Amin Nasiri
Abstract
In order to evaluate self and cross-incompatibility in two ecotypes of Damask rose (Rosa damascena Mill.), this experiment was performed based on randomized complete block design (RCBD) with four replications at the research field of Tarbiat Modares University in 2012-2013. In this ...
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In order to evaluate self and cross-incompatibility in two ecotypes of Damask rose (Rosa damascena Mill.), this experiment was performed based on randomized complete block design (RCBD) with four replications at the research field of Tarbiat Modares University in 2012-2013. In this experiment, twice pollinations were performed 24-hour interval, after doing in vitro testing pollen grain germination. Pollens collected from Kashan and Azaran ecotypes and Dog rose (Rosa canina) were applied as pollinizer for self- and cross-artificial pollination on both ecotypes. Determination of self and cross incompatibility were conducted with two methods of field controlled pollination and microscopic studies. Results obtained from percentage of hip development, hip weight and length, as well as seed production per hip showed that cross pollination with Dog rose pollen had the highest compatibility, comparing to pollen of Kashan and Azaran ecotypes that showed incompatibility. Also, twice pollinations had better result in comparison to one-time pollination. Field results were supported by microscopic studies based on growing pollen tube in style. According to growing pollen tube in style, just pollen tube of Dog rose could get to end of style. The concentration of acid boric (50 mg/kg) showed the highest pollen germination after 24 h, while 200 mg/kg acid boric increased pollen tube growth. According to the present results, selection of suitable pollinizer and repetition of pollination were important factors affecting Damask rose crossing, as twice controlled cross pollination by Dog rose pollen had most appropriate results on hip characteristics.