Mehrdad Rasouli; Alireza Noroozisharaf
Abstract
The use of compounds that can improve plant tolerance to abiotic environmental stress, including salinity, is important. To evaluate the adjustment of salinity stress using humic acid (HA), a factorial layout is conducted based on a complete randomized design with three replications on the Hypericum ...
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The use of compounds that can improve plant tolerance to abiotic environmental stress, including salinity, is important. To evaluate the adjustment of salinity stress using humic acid (HA), a factorial layout is conducted based on a complete randomized design with three replications on the Hypericum perforatum L. as an Iranian medicinal plant are collected from Hamadan province in the tissue culture laboratory of the Faculty of Agriculture, Sayyed Jamaleddin Asadabadi University during 2019. Experimental treatments include NaCl as salinity at three levels (such as 0, 50, and 100 mmol.L-1) and HA at four levels (control, 25, 50, and 100 mg.L-1). Results indicate that the plants treated with 50 mg.L-1 HA without salinity have had the highest germination percentage (98.65), germination rate (38.94) and root length (34.21 mm). The highest plant height, fresh and dry weight of both shoots and roots and total chlorophyll are obtained under control conditions and HA with a concentration of 100 mg.L-1. On the contrary, the highest amount of phenol in the treatment is 50 mmol.L-1 salinity and HA with a concentration of 50 mg.L-1. In the treatment of 100 mmol.L-1 salinity and HA with a concentration of 50 mg.L-1, the highest amount of total antioxidants (99.77 mg/g FW) and total flavonoids (2.39 mg/Qe g FW) and the lowest amount of hydrogen peroxide (9.12 µg/g FW) are obtained. Results show that the mitigating effect, especially the application of HA of 50 mg.L-1 levels, can affect the physiological processes and morphological traits of the Hypericum perforatum L. under salinity stress.
Hamid Zargari; Alireza Talaie; Yahya Dehghani Shurki; Vahid Abdossi
Abstract
One of the problems faced by Phoenix dactylifera L growers in different parts of the country is the lack of a favorable fruit set for tissue culture seedlings of some cultivars recently. This study is conducted to investigate the effects of pollen of different cultivars on the fruit set of Phoenix dactylifera ...
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One of the problems faced by Phoenix dactylifera L growers in different parts of the country is the lack of a favorable fruit set for tissue culture seedlings of some cultivars recently. This study is conducted to investigate the effects of pollen of different cultivars on the fruit set of Phoenix dactylifera L tissue culture and offshoot palms of Barhi, Piaroom, and Mazafati cultivars in the garden of Jahrom Agricultural Research Station. During the years 2018 - 2019, in a factorial experiment, three factors have been performed in three replications with a randomized complete block design. The first factor involves different pollen with five levels (i.e., codes 7001 (control), 7005, 7013, 7030, and Boyer 11 obtained from tissue culture (B11)) with the second factor being the cultivar type in three levels (Barhi, Pyaram, and Mazafati), and the third one, the type of seedlings, all applied on 90 ten-year-old trees in two levels (tissue culture and offshoot). Results show that the highest percentage of natural fruit set has been observed in Mazafati cultivar with 7013 and 7030 pollens and 7013 pollen in Barhi cultivar. The lowest percentage of natural fruit set belongs to tissue culture method in Barhi cultivar with pollen 7001 (control). In general, 7013 and 7030 pollens have been identified as the most suitable pollinator base to increase the percentage of normal fruit set and reduce parthenocarpic and fallen fruits of Barhi, Piaroom, and Mazafati cultivars obtained from tissue culture and offshoot.