Gholamhasan Movahed; Nima Ahmadi; Ahmad Moieni
Abstract
In order to evaluate the responses of two ecotypes of Damask rose (Rosa damascena) to exogenous ethylene, an experiment was conducted based on a completely randomized design (CRD) at the laboratory of Postharvest Physiology, Tarbiat Modares University, Tehran, Iran. Damsk rose cut stems containg flower ...
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In order to evaluate the responses of two ecotypes of Damask rose (Rosa damascena) to exogenous ethylene, an experiment was conducted based on a completely randomized design (CRD) at the laboratory of Postharvest Physiology, Tarbiat Modares University, Tehran, Iran. Damsk rose cut stems containg flower buds and/or open flowers, single buds, or multiple flowers were exposed to exogenous ethylene for 24 h. Plant organs were placed into hearmatically seald glass chambers, where ethylen was injected, to achieve 0, 1, 2, 3, and 4 µl/l concentrations. Biocehmival characteristics, enzyme activities and morphological characteristics including flower life, contents of anthocyanin, flavonoide, and proline were determind in Kashan and Azaran ecotypes of Damask rose. The lowest psotharvest life was observed at 3 and 4 μl concentration of ethylene and the highest abscission was observed in Azaran ecotypes at a concentration of 4 μl/l of ethylene after four days. Anthocyanin and proline content and catalase activity reduced in both ecotypes studied. For both ecotypes, the lowest content of anthocyanin, proline and the lowest catalase activity (48/77 ΔA 240/mg protein) were observed in those treated with ethylene at 4 µl/l. The highest malondialdehyde (3.63 µmol/g petal fresh weight) was found in Azaran ecotype flowers treated with 4 µl/l ethylene concentration. Decrease of total protein in Kashan flowers (0.32 mg/g fresh petal) was much less than that in Azaran flowers (0.2 mg/g fresh petal). Based on these results, Kashan could be recommended as a tolerant ecotype to be used in breeding programs.
Gholamhasan Movahed; Nهئش Ahmadi; Ahmad Moieni; Amin Nasiri
Abstract
In order to evaluate self and cross-incompatibility in two ecotypes of Damask rose (Rosa damascena Mill.), this experiment was performed based on randomized complete block design (RCBD) with four replications at the research field of Tarbiat Modares University in 2012-2013. In this ...
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In order to evaluate self and cross-incompatibility in two ecotypes of Damask rose (Rosa damascena Mill.), this experiment was performed based on randomized complete block design (RCBD) with four replications at the research field of Tarbiat Modares University in 2012-2013. In this experiment, twice pollinations were performed 24-hour interval, after doing in vitro testing pollen grain germination. Pollens collected from Kashan and Azaran ecotypes and Dog rose (Rosa canina) were applied as pollinizer for self- and cross-artificial pollination on both ecotypes. Determination of self and cross incompatibility were conducted with two methods of field controlled pollination and microscopic studies. Results obtained from percentage of hip development, hip weight and length, as well as seed production per hip showed that cross pollination with Dog rose pollen had the highest compatibility, comparing to pollen of Kashan and Azaran ecotypes that showed incompatibility. Also, twice pollinations had better result in comparison to one-time pollination. Field results were supported by microscopic studies based on growing pollen tube in style. According to growing pollen tube in style, just pollen tube of Dog rose could get to end of style. The concentration of acid boric (50 mg/kg) showed the highest pollen germination after 24 h, while 200 mg/kg acid boric increased pollen tube growth. According to the present results, selection of suitable pollinizer and repetition of pollination were important factors affecting Damask rose crossing, as twice controlled cross pollination by Dog rose pollen had most appropriate results on hip characteristics.