Nooshin Kazemi; Fariborz Zaree Nahandi; Ali Akbar Habashi; Wahab Asadi
Abstract
This study was aimed to production of healthy primary pear plants for propagation and establishment of a high health maternal orchard in 2014 at Agriculture Biotechnology Research Institute of Iran (ABRII). The experiments were conducted by evaluate the effectiveness of the chemotherapy treatments (Ribavirin ...
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This study was aimed to production of healthy primary pear plants for propagation and establishment of a high health maternal orchard in 2014 at Agriculture Biotechnology Research Institute of Iran (ABRII). The experiments were conducted by evaluate the effectiveness of the chemotherapy treatments (Ribavirin (0, 20, 40 and 80 mg/l) and the sizes of apical meristems culturing (less than 0.2 mm, between 0.2 to 0.7 mm and larger than 0.7 mm) on virus eradicate (ACLSV, ASPV, ASGV) from seven pear cultivars ("Abate Fetal", "Beiruti", "Coscia", "Dargazi", "Louise Bonne", "Mellina" and "Spadona"). At first maternal samples were tested for ACLSV, ASGV and ASPV by RT-PCR and all explants had been infected with all three viruses except Fettel and Beirut that were free from ASPV. We performed the chemotherapy treatments then meristems were cultured and grown in vitro. Regenerated shoots from meristem were tested by RT-PCR for all three viruses. The results showed that increasing the concentration of ribavirin and reducing the size of cultivated meristem was effective on all the three viruses’ elimination from explants. Also the responses of studied cultivars and virus varieties were different in treatments of this research. Samples that were diagnosed virus free by using RT-PCR, were proliferated, rooted and transferred into the pots to be used for later propagation and establishment of maternal orchard.
Hossein Rabbi Angorani; Jaber Panahandeh; Sahebali Boland nazar; Jalal Saba; fariborz Zare Nahandi
Abstract
Oxidative stress is one of the most important consequences of drought stress. Salicylic acid is a phenolic compound which serves as a growth regulator in the induction of resistance to drought. In order to investigate the role of salicylic acid on some anti-oxidant enzymes and some biochemical attributes ...
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Oxidative stress is one of the most important consequences of drought stress. Salicylic acid is a phenolic compound which serves as a growth regulator in the induction of resistance to drought. In order to investigate the role of salicylic acid on some anti-oxidant enzymes and some biochemical attributes of medicinal pumpkin under drought stress, an experiment was designed 2014 in Zanjan University’s Research Farm, Iran. The study was arranged as factorial experiment based on a randomized complete block design with three replications including drought stress at four level; control, mild stress, moderate stress, and severe stress have been arranged in four levels including (100, 85, 70 and 55% FC). Salicylic acid treatments included salicylic acid were applied as foliar application spray in four levels: 0 mg/l (solution spray with distilled water), 0.5, 1, and 1.5 mg/l. Measured traits included peroxidase, catalase, Beta-sitosterol and oil yield proline, electrolyte leakage, malondialdehyde (membrane peroxidation index) content. The results showed that increasing drought stress levels reduced the oil yield, but in contrast, increasing the Beta-sitosterol, membrane peroxidation, electrolyte leakage, anti-oxidant enzymes such as catalase and peroxidase, while application of salicylic acid at 0.5 and 1 mg/l decreased oxidative stress, membrane peroxidation and Electrolyte leakage through increasing the anti-oxidant enzymes activities such as catalase and peroxidase and proline which led to a balanced rise in oil yield and Beta-sitosterol in mild and moderated stress. These results show an increase in plant resistance to drought as a result of salicylic acid application.
Fariborz Zaare-Nahandi; Nilufar Mohamadi Silabi
Abstract
Flowering and fruit production of ‘Nagami’ kumquat is somewhat different from other citrus species because usually have three flowering peak in a year which can lead to producing fruits with different quality on a tree. Considering the proper effect of paclobutrazol on raising citrus flowering, ...
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Flowering and fruit production of ‘Nagami’ kumquat is somewhat different from other citrus species because usually have three flowering peak in a year which can lead to producing fruits with different quality on a tree. Considering the proper effect of paclobutrazol on raising citrus flowering, a pot experiment with three levels of paclobutrazol (zero, 0.1 and 0.5 g ai per tree) and six replications was conducted as a complete randomized block design. The results showed that paclobutrazol had effective effect on kumquat flowering and fruit set. Effect of paclobutrazol on shoot growth before and after the flowering period and the number of shoots after flowering period was significant and led to reducing them. All paclobutrazol treatments led to early flowering, increasing the number of first and second flower flush and fruit production. In terms of fruit quality, significant decrease in the length, diameter/length ratio and acidity of fruits was observed. In general, paclobutrazol caused a marked increase in fruit production of ‘Nagami’ kumquat, while retained most of the desirable traits of fruit.
Yalda Ghazi-Vakili; Ali Reza Motallebi-azar; Fariborz Zare Nahandi; Naser Mahna
Abstract
Due to the importance of production and propagation of virus-free potato plants, some of the factors involved in the propagation of potato (plantlet production and virus-free in vitro microtuberization) has been studied. In most reports, adding a mixture of amino acids play an important role in plant ...
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Due to the importance of production and propagation of virus-free potato plants, some of the factors involved in the propagation of potato (plantlet production and virus-free in vitro microtuberization) has been studied. In most reports, adding a mixture of amino acids play an important role in plant tissue culture and development of the explants in many cases increase. This experiment was conducted in a completely randomized design with three replications to investigate the effects of glutamine, arginine, asparagine, cysteine and their combinations on microtuberization of potatoes. Lateral buds from in vitro shoots were cultured on MS medium under sterile conditions. Cultures were kept in constant darkness and temperatures of 17 ± 2°C in a germinator. During the first month of culture microtuberization rate and after two months other microtuberization traits such as microtubers weight was measured. Analysis of variance showed that all traits except microtuber without rest were significantly affected by four amino acids. In this study, arginine in combination with asparagine had a positive role in improving the most important characteristics such as weight and microtuberization rate.