Mahdi Sadeghi Raviz; Nourollah Ahmadi; Naser Safaie; Iman Rahmani
Abstract
The effect of methyl jasmonate (MeJA) vapor treatment on antioxidant enzymes activities and vase life of gerbera cut flower was investigated in this experiment. This research was conducted as a split-plot experiment based on completely randomized design with three replications at the laboratory of the ...
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The effect of methyl jasmonate (MeJA) vapor treatment on antioxidant enzymes activities and vase life of gerbera cut flower was investigated in this experiment. This research was conducted as a split-plot experiment based on completely randomized design with three replications at the laboratory of the postharvest physiology, College of Agriculture, Tarbiat Modares University in 2013. Gerbera flowers were harvested at early morning from a commercial greenhouse and cut flower stems were put in a preservative solution containing 200 mg/L 8- hydroxyquinoline sulfate and sucrose 3%, exposed to 0, 0.1, 0.2 and 0.3 µlL−1 MeJA with 20 µlL−1 ethanol and control for 24 h. The results showed that MeJA significantly increased vase life and improved postharvest characteristics of cut gerbera flowers. MeJA 0.2 µlL−1 treatment extended the vase life to 15.67 days, compared to the control and ethanol treatments. Minimum of petal wilting symptoms were revealed in samples treated with MeJA. The lowest malondialdehyde and lipid peroxidation rates were also observed in MeJA treatments that they had a significant difference with ethanol and control. MeJA treatments resulted in increasing antioxidant enzymes activity and protein content compared with ethanol or control.
Gholamhasan Movahed; Nima Ahmadi; Ahmad Moieni
Abstract
In order to evaluate the responses of two ecotypes of Damask rose (Rosa damascena) to exogenous ethylene, an experiment was conducted based on a completely randomized design (CRD) at the laboratory of Postharvest Physiology, Tarbiat Modares University, Tehran, Iran. Damsk rose cut stems containg flower ...
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In order to evaluate the responses of two ecotypes of Damask rose (Rosa damascena) to exogenous ethylene, an experiment was conducted based on a completely randomized design (CRD) at the laboratory of Postharvest Physiology, Tarbiat Modares University, Tehran, Iran. Damsk rose cut stems containg flower buds and/or open flowers, single buds, or multiple flowers were exposed to exogenous ethylene for 24 h. Plant organs were placed into hearmatically seald glass chambers, where ethylen was injected, to achieve 0, 1, 2, 3, and 4 µl/l concentrations. Biocehmival characteristics, enzyme activities and morphological characteristics including flower life, contents of anthocyanin, flavonoide, and proline were determind in Kashan and Azaran ecotypes of Damask rose. The lowest psotharvest life was observed at 3 and 4 μl concentration of ethylene and the highest abscission was observed in Azaran ecotypes at a concentration of 4 μl/l of ethylene after four days. Anthocyanin and proline content and catalase activity reduced in both ecotypes studied. For both ecotypes, the lowest content of anthocyanin, proline and the lowest catalase activity (48/77 ΔA 240/mg protein) were observed in those treated with ethylene at 4 µl/l. The highest malondialdehyde (3.63 µmol/g petal fresh weight) was found in Azaran ecotype flowers treated with 4 µl/l ethylene concentration. Decrease of total protein in Kashan flowers (0.32 mg/g fresh petal) was much less than that in Azaran flowers (0.2 mg/g fresh petal). Based on these results, Kashan could be recommended as a tolerant ecotype to be used in breeding programs.
Gholamhasan Movahed; Nهئش Ahmadi; Ahmad Moieni; Amin Nasiri
Abstract
In order to evaluate self and cross-incompatibility in two ecotypes of Damask rose (Rosa damascena Mill.), this experiment was performed based on randomized complete block design (RCBD) with four replications at the research field of Tarbiat Modares University in 2012-2013. In this ...
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In order to evaluate self and cross-incompatibility in two ecotypes of Damask rose (Rosa damascena Mill.), this experiment was performed based on randomized complete block design (RCBD) with four replications at the research field of Tarbiat Modares University in 2012-2013. In this experiment, twice pollinations were performed 24-hour interval, after doing in vitro testing pollen grain germination. Pollens collected from Kashan and Azaran ecotypes and Dog rose (Rosa canina) were applied as pollinizer for self- and cross-artificial pollination on both ecotypes. Determination of self and cross incompatibility were conducted with two methods of field controlled pollination and microscopic studies. Results obtained from percentage of hip development, hip weight and length, as well as seed production per hip showed that cross pollination with Dog rose pollen had the highest compatibility, comparing to pollen of Kashan and Azaran ecotypes that showed incompatibility. Also, twice pollinations had better result in comparison to one-time pollination. Field results were supported by microscopic studies based on growing pollen tube in style. According to growing pollen tube in style, just pollen tube of Dog rose could get to end of style. The concentration of acid boric (50 mg/kg) showed the highest pollen germination after 24 h, while 200 mg/kg acid boric increased pollen tube growth. According to the present results, selection of suitable pollinizer and repetition of pollination were important factors affecting Damask rose crossing, as twice controlled cross pollination by Dog rose pollen had most appropriate results on hip characteristics.
Azam Ranjbar; Nourollah Ahmadi
Abstract
This research was conducted to evaluate the effects of 1-MCP and ethylene on extending the display quality and increasing vase life of cut carnation flowers in a completely randomized design with three replicationsat the laboratory of the postharvest physiology, Tarbiat Modares Universityin 2014. First, ...
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This research was conducted to evaluate the effects of 1-MCP and ethylene on extending the display quality and increasing vase life of cut carnation flowers in a completely randomized design with three replicationsat the laboratory of the postharvest physiology, Tarbiat Modares Universityin 2014. First, cut flowers were treated with 0, 0.5, 1 and 1.5 µl l-1 of 1-MCP for 24 h and then exposed to 1 µl l-1 ethylene for 16 h. The results showed that 1-MCP had significant effect on the physiological and biochemical characteristics and the activity of antioxidant enzymes. Maximum of vase life and the amount of chlorophyll and anthocyanin were revealed in samples treated with 1 µl l-1 1-MCP. Also, the highest peroxidase enzyme activity was observed in treatment of 1 µl l-1 1-MCP which had significant difference with other treatments. The highest and lowest catalase and superoxide dismutase activities were found in 1.5 µl l-1 1-MCP and control, respectively. In conclusion, 1-MCP treatment resulted in extending vase life and increasing activity of antioxidant enzymes in cut carnation cv. ‘Grand Slam’ by suppression of ethylene action.
seyedeh samaneh hosseini; Noorollah Ahmadi; Abbas Yadollahi
Abstract
This research was conducted to determine the optimum bud or flower developing stage for pollen collection and a proper pollen germination medium and to evaluate the effect of storage temperature and different concentrations of putrescine on pollen viability and germination of two genotypes of damask ...
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This research was conducted to determine the optimum bud or flower developing stage for pollen collection and a proper pollen germination medium and to evaluate the effect of storage temperature and different concentrations of putrescine on pollen viability and germination of two genotypes of damask rose (‘Azaran’ and ‘Kashan’). The main experiment was performed based on a factorial design, with 16 treatment and 4 replications. Factors studied included four levels of putrescine (concentrations zero, 0.25, 0.5 and 2.5 mM) and four levels of pollen storage temperatures (25, four, -20 and -80°C). The best stage for collecting pollen grain was semi-open bud stage and the optimum medium containing 300 μg/l CaNO3, 200 μg/l MgSO4, 100 μg/l KNO3, 100 μg/l H3BO3, 15 percent sucrose and 1.2 percent agar. In ‘Azaran’ genotype, maximum and minimum pollen germination rate was obtained in media containing 0.25 and 2.5 mM putrescine, respectively. Storage condition of pollen grains resulted in decreasing the pollen germination rate in ‘Kashan’ genotype, while storage of pollen grains at -80°C revealed higher germination rate on medium containing 0.25 mM putrescine. As a result, maximum pollen germination percentage was obtained by storage of pollens in low temperature (-80°C) and in media containing low concentrations of putrescine.
Nourollah Ahmadi
Abstract
Ethylene as a plant hormone plays various functions in a variety of physiological phenomena including seed germination, root initiation on stem cutting, senescence and organs abscission. In our previous investigation, five rose genotypes were identified showing high-sensitivity or low-sensitivity to ...
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Ethylene as a plant hormone plays various functions in a variety of physiological phenomena including seed germination, root initiation on stem cutting, senescence and organs abscission. In our previous investigation, five rose genotypes were identified showing high-sensitivity or low-sensitivity to exogenous ethylene treatment. The purpose of this study was to evaluate the rooting capacity and bud growth in stem cuttings of these genotypes. Although significant differences were found in rooting ability of 1-cm cuttings but 2-cm cuttings did not show any difference. IBA treatment did not affect root formation in low sensitive or high sensitive plants. The highest and lowest mean rooting time belong to genotypes '76.67' and 'Vanilla' respectively. There were significant differences in plant height and dry weight of shoots and roots of genotypes. This study showed that the rooting capacity was not affected by degree of plant sensitivity to ethylene.