In order to study the effect of bio fertilizers and putrescine on yield and some traits of wheat (Triticum aestivum L. (at various irrigation levels, a factorial experiment was conducted based on randomized complete block design with three replications at the research farm of University of Mohaghegh Ardabili, during 2018-2019. Factors were included irrigation (full irrigation as control, irrigation withholding in 50% of heading and booting stages), bio fertilizers (no bio fertilizer, application of mycorrhiza, both application Psedomunas and Flavobacterim, application of mycorrhiza with Psedomunas and Flavobacterim) and putrescine foliar application (foliar application with water as control, foliar application 0.5 and 1 mM of putrescine). Means comparison showed that maximum of relative water content of flag leaf; chlorophyll index, stomatal conductance and quantum yield were obtained in full irrigation, application of mycorrhiza with Psedomunas and Flavobacterim and foliar application 1 mM of putrescine. Foliar application 1 mM of putrescine increased grain yield of about 8.5% in comparison with no application putrescine. Also, the highest grain yield (799.44 and 746.38 g.m-2) were obtained with both application Psedomunas and Flavobacterim, both application of mycorrhiza with Psedomunas and Flavobacterim respectively under full irrigation conditions and the lowest it (403.42 g.m-2) was obtained in under irrigation withholding in booting stage, no application of bio fertilizer. It seems that application of putrescine and bio fertilizers can increase grain yield of wheat under water limitation conditions due to improving some physiological traits.