In order to investigate the effects of irrigation intervals on pigments and biochemical components of four grapevine cultivars (Yaghooti-e-Ghermez, Askari, Sahebi and Keshmeshi-e-Sefid), an experiment was conducted with five irrigation treatments (3, 6, 9, 12 and 15 days interval) in four replications in greenhouse in 2012. The results of this experiment showed that irrigation intervals have effected on physiological and biochemical factors of grapevine cultivars. The amount of chlorophyll a, b and total chlorophyll in four cultivars were reduced with increasing irrigation intervals, and maximum reduction was observed in 15 days interval irrigation in four cultivars. The maximum and the minimum of chlorophyll a, b, total chlorophyll and carotenoids content were observed in ‘Sahebi’ at 6 days interval irrigation and ‘Askari’ at 15 days, respectively. Total soluble sugars, phenolic compounds and proline contents of leaves in all cultivars increased and leaf relative water content decreased with increasing diurnal irrigation. The maximum leaf relative water content and proline was observed in Sahebi cultivar and the minimum value was belonged to Yaghooti-e-Ghermez and Askari cultivars. Based on the results of this study, it seems that Sahebi cultivar had more tolerant in increasing irrigation intervals compared to Yaghooti-e-Ghermez, Askari and Keshmeshi-e-Sefid cultivars.