Maryam Forough; Saeid Navabpour; Esmaeil Ebrahimie; Ali Akbar Ebadi; Davood Kiani
Abstract
In this study, in order to investigate the response to salinity stress, seedlings of two rice kinds, Hashemi and its advanced mutant line, have exposed to 100 mM NaCl as a salinity stress in an experiment, conducted in 2017 at Gorgan University of Agricultural Sciences and Natural Resources, Iran. For ...
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In this study, in order to investigate the response to salinity stress, seedlings of two rice kinds, Hashemi and its advanced mutant line, have exposed to 100 mM NaCl as a salinity stress in an experiment, conducted in 2017 at Gorgan University of Agricultural Sciences and Natural Resources, Iran. For the biochemical investigation, root sampling is performed during three and six days after the salinity stress treatment. The experiment is conducted as a split plot with randomized complete block design with three replications in hydroponic culture. Under salinity stress, the sodium ion content in both genotypes’ roots has increased significantly, while this trend is much lower in the root of mutant genotype than wild type. The induced oxidative stress of salinity stress is measured by the amount of hydrogen peroxide, indicating that the wild type is under higher oxidative stress which is confirmed by the higher amount of malondialdehyde. Evaluation of antioxidant enzymes’ activity include superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase. It reveals a significant rise in the root of the mutant genotype. Overall, this study shows that mutation in the rice genotype leads to salt tolerance, compared to the wild type, through promoting the activity of oxidative enzymes and the synthesis of some osmolytes in the root tissue.
Omid Sadeghipour
Abstract
In order to investigate the role of methyl jasmonate on salinity tolerance of cowpea, a factorial pot experiment was conducted in the basis of completely randomized design with three replications in the Rey region in 2015. Treatments included three levels of seed soaking in methyl jasmonate solution ...
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In order to investigate the role of methyl jasmonate on salinity tolerance of cowpea, a factorial pot experiment was conducted in the basis of completely randomized design with three replications in the Rey region in 2015. Treatments included three levels of seed soaking in methyl jasmonate solution (0, 25 and 50 µM) for 20 hours and three levels of salinity (0, 50 and 100 mM NaCl). The results showed that by increasing salinity levels, lipids peroxidation, antioxidant enzymes activity (superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase) and Cl- concentration in the leaves increased, while the concentration of N, Ca2+ and Mg2+ decreased as well as K+/Na+ ratio. These changes eventually led to decreasing seed yield. Seed treatment with methyl jasmonate had not significantly effect on any measured traits under normal conditions, however this treatment (especially 50 µM) under salinity stress conditions caused further activity of antioxidant enzymes, declining lipids peroxidation, reducing the concentration of Cl- in the leaves, increasing the concentration of N, Ca2+ and Mg2+ as well as K+/Na+ ratio, and finally improved seed yield. These findings suggest that methyl jasmonate plays a pivotal role in inducing salinity tolerance of cowpea plants via enhancing antioxidant enzymes activity and altering nutrients composition.
Fariba Rezaei Aderyani; Ayatollah Rezaei; Yavar Sharafi
Abstract
Salinity is one of the most important plant growth limiting factors, which using the anti-stress materials is considered as an important approach in moderating its effects on plants. In this research, the date plum seedlings response to salinity stress and two compounds putrescine and chitosan to reduce ...
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Salinity is one of the most important plant growth limiting factors, which using the anti-stress materials is considered as an important approach in moderating its effects on plants. In this research, the date plum seedlings response to salinity stress and two compounds putrescine and chitosan to reduce salinity stress was studied. The experiment was conducted as a factorial with three levels of sodium chloride (0, 30 and 60 mM), and five treatments of control, putrescine (1 and 2 mM) and chitosan (0.25 and 0.5%), based on a completely randomized design with four replications at Shahed University in 2016. The results showed that date plum was sensitive to salt stress in the early stages of growth and sodium chloride salinity stress resulted in significant decrease in vegetative traits such as fresh matter weight and length of shoots and roots as well as leaf chlorophyll content in compared to 0 salinity. In addition, the leaf blight percentage, sodium content, electrolyte leakage and malondialdehyde content were increased in plants treated with salinity. Putrescine treatment, especially at 2 mM concentration effectively reduced the effects of salinity on date plum seedlings and improved vegetative growth of stem and root, while chitosan had no significant effect in reducing the harmful effects induced by salt stress in this experiment. According to the results, Diospyros lotus seedlings were sensitive to salinity and using treatments such as putrescine is effective in increasing its resistance to salinity stress.
Leila Mohammadi; Saeed Rizi; Rahim Barzegar
Abstract
To evaluate the effect of arbuscular mycorrhiza (Glomus mosseae) under salt stress on physiological and some nutrient absorption in New Guinea Impatiens (Impatiens hawkeri), an experiment established in complete random design with three replications (three pots for each replication) in greenhouse in ...
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To evaluate the effect of arbuscular mycorrhiza (Glomus mosseae) under salt stress on physiological and some nutrient absorption in New Guinea Impatiens (Impatiens hawkeri), an experiment established in complete random design with three replications (three pots for each replication) in greenhouse in Shahrekord University in 2014. Treatments were inoculation of substrate with three levels of arbuscular mycorrhiza (0, 8 and 16 percent, v/v) and sodium chloride (0, 15 and 30 Mm). The substrate medium was included 50 percecnt of peat moss, 40 percent of perlite and 10 percent of rice husk (v/v). The mycorrhizal inoculation done with transplanting and salt stress treatment was applied with irrigation water after stablishment of transplants. Some traits such as nitrogen, phosphorous, potassium, sodium and proline content, dry and fresh root weight and root colonization percentage were evaluated. The results showed that 16 percent of mycorrhiza treatment had significant effect on nitrogen (2.31 percent) and phosphorous (0.339 percent) and it’s interaction with 30 Mm of salt stress had significant effect on proline content (0.754 micromol/gfw) and root colonization (35 percent) percentage. Based on this research the difference between mycorrhiza treated plants and other treatments was significant and it seems that the application of mycorrhiza in medium, can increase salt tolerance in New Guinea Impatiens by effect on some physiological traits.
Morteza Baziar; Ali Bandehagh; Davood Faraj Zadeh
Abstract
This study was designed to investigate the effect of P. fluorescens FY32 inoculation on the activities of some antioxidant enzymes and ion concentration of two canola cultivars (Sarigol and Hyola308) under salinity stress (150 and 300 mM NaCl) in the greenhouse condition (fall 2012, Dept. of Plant breeding ...
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This study was designed to investigate the effect of P. fluorescens FY32 inoculation on the activities of some antioxidant enzymes and ion concentration of two canola cultivars (Sarigol and Hyola308) under salinity stress (150 and 300 mM NaCl) in the greenhouse condition (fall 2012, Dept. of Plant breeding and Biotechnology, Faculty of Agriculture, University of Tabriz). The experimental design consisted of three factors replicated three times in a split-split plot design, with salinity as main factor. Data analysis showed that salt stress has significant effect on antioxidant enzyme activity (catalase, peroxidase and polyphenol oxidase). Concentration of sodium and chloride ions increased with increasing salinity, but this increase was little in the case of inoculated plants. The decrease in potassium content was smaller in inoculated plants in comparison with control. Hyola308 had the lowest reduction in dry matter and was tolerant cultivar in this experiment. Thia cultivar as a tolerant cultivar had low level of antioxidant activities and accumulation of sodium and chloride ions and also showed high concentration of potassium.
Hamid Rahnemoun; Farid Shekari; Jalil Dejampour; Mohammad Bagher Khorshidi
Abstract
To evaluate morphological and biochemical changes of almond (Prunus dulcis Mill.) under salt stress conditions and determine relationship among accumulation of Na and Cl with these traits, an experiment was conducted based on completely randomized design with four treatments including 0 (control), 25, ...
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To evaluate morphological and biochemical changes of almond (Prunus dulcis Mill.) under salt stress conditions and determine relationship among accumulation of Na and Cl with these traits, an experiment was conducted based on completely randomized design with four treatments including 0 (control), 25, 50 and 75 mM of pure sodium chloride and three replications from 2010 to 2012. The seed of healthy almond genotypes grown in natural saline areas of East-Azerbaijan province were used as the experimental material in this study. Salt treatments were influenced during eight weeks following the growth and appearance of 15 – 20 leaves on each seedling. The results showed that the threshold of marginal leaf scorch symptoms was in relation with an accumulation of 9–11 mg/g DW Na. Also, mean of free proline content increased from 37.7 to 117.9 µmol.g-1 FW linearly and significantly in parallel with the accumulation of Na and Cl in the leaves. Under same conditions, catalase and ascorbate-peroxidase activity increased significantly; however peroxidase activity increased up to 50 mM salinity but decreased subsequently at higher levels of salt.