Mina Amani; Saeideh Alizadeh-Salteh; Mohsen Sabzi nojadeh; Mehdi Younessi Hamzekhanlu
Abstract
Objective: Taking advantage of the symbiotic relationship between plants and Trichoderma fungi is one of the ways to reduce water stress in plants. The present study was conducted in order to investigate the effect of Trichoderma fungus on the antioxidant properties of the basil medicinal plant (Ocimum ...
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Objective: Taking advantage of the symbiotic relationship between plants and Trichoderma fungi is one of the ways to reduce water stress in plants. The present study was conducted in order to investigate the effect of Trichoderma fungus on the antioxidant properties of the basil medicinal plant (Ocimum basilicum L.) under water stress conditions.
Methods: This experiment was carried out in the greenhouse of the Department of Horticulture Sciences of Ahar Faculty of Agriculture and Natural Resources and laboratory studies in the basic and general laboratories of Ahar Faculty of Agriculture and Natural Resources (University of Tabriz) in 2019 in a factorial manner based on a randomized complete block design with three repetitions. The treatments included different levels of water deficit stress, including severe stress (25% of field capacity), moderate stress (50% of field capacity), mild stress (75% of field capacity), and no stress (100% of field capacity). Each pot contained a commercial mushroom species Trichoderma harzianum Na-lac with concentrations of 109 and 106 spores per milliliter as the inoculum.
Results: The results showed that the amount of malondialdehyde, catalase and peroxidase, antioxidant activity and total flavonoid increased with the application of dehydration stress. The concentration of 106 spores per milliliter had a better impact on improving the mentioned indicators. Based on this, Trichoderma fungus proves effective when improving the antioxidant status of plants under drought stress and can prevent the effects of oxidative stress in plants by reducing the oxygen free radicals produced.
Conclusion: The results indicated that the use of Trichoderma mushroom in comparison with the control (without inoculation with the mushroom) can be a suitable tool to improve the physiological traits and antioxidant activities in the conditions drought stress.
Amir Mizani; jafar masoud sinaki; Shahram Rezvan; Mohammad Abedini-Esfahlani; Ali Damavandi
Abstract
This experiment works on the physiological, growth, and yield responses of sesame to the application of potassium nano-fertilizers, chitosan, and fulvic acid under drought stress in Semnan Agricultural and Natural Resources Research Center as a factorial split-plot based on randomized complete block ...
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This experiment works on the physiological, growth, and yield responses of sesame to the application of potassium nano-fertilizers, chitosan, and fulvic acid under drought stress in Semnan Agricultural and Natural Resources Research Center as a factorial split-plot based on randomized complete block design with three replications in 2019. The experimental treatments include drought as the main plot (normal irrigation, irrigation cut offin 70 and 60 BBCH as moderate, and severe stress, respectively) and potassium nano-fertilizer (0, 1.5, and 2.5 per thousand) and foliar application (control, chitosan, fulvic acid, and 50% of chitosan+ fulvic acid) as sub-plots.Application of potassium nano-fertilizer under normal and stress conditions significantly cuts chlorophyll b and total, leaf area index, number of capsules, 1000-seed weight, and oil percentage.The highest grain yield belongs to the application of 1.5 and 2.5 per thousand nano fertilizers, using fulvic acid alone or in combination with 50% chitosan (2516, 2277.5, 2506.6, and 2313.2 kg/ha, respectively). The highest oil content has been in 1.5 and 2.5 per thousand nano fertilizer application under normal irrigation, which has increased 13.2% and 15.4%, respectively. Foliar application of fulvic acid with nano potassium fertilizer (1.5 per thousand) under severe drought stress show the highest activity of antioxidant enzymes catalase, peroxidase, ascorbate peroxidase, and superoxide dismutase. In general, the results show that the application of 1.5 per thousand nano potassium fertilizer and combined foliar application of chitosan and fulvic acid under severe drought by improving physiological traits, manage to modify the adverse effects of drought, increasing growth, yield, and quality indices of sesame.
Ali Yazdan panah gohari; Marzieh Ghanbari Jahromi; Vahid Zarrin nia
Abstract
Mycorrhiza is a common group of microorganisms that can establish symbiotic relations with plants. To evaluate symbiosis and compatibility between a collection of chicory seeds and mycorrhizal fungi, through inoculation, a factorial experiment is set up in a randomized complete block design. Two batches ...
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Mycorrhiza is a common group of microorganisms that can establish symbiotic relations with plants. To evaluate symbiosis and compatibility between a collection of chicory seeds and mycorrhizal fungi, through inoculation, a factorial experiment is set up in a randomized complete block design. Two batches of chicory seed (Ardabil and Fars) are inoculated by two species of mycorrhizal fungi (Glomus mosseae and Glomus intraradices) with the control group of the seeds not being inoculated at all. Three replicates have been arranged in a greenhouse. The research aims at examining how the two species of mycorrhizal fungi affect several plant traits and the amounts of phytochemical compounds (i.e. phenol, flavonoids, antioxidant activity, chlorophyll a and b, total chlorophyll), fresh and dry weights of roots and root length. It also deals with chicory roots’ ability to absorb several elements. Results show that seed batch and mycorrhizal fungi have significant impacts on vegetative traits, chlorophyll content, phenol, total flavonoids, and antioxidant activity in plants. The highest amounts of total phenol production (3.77 mg/g fresh weight), total flavonoids (1.21 mg/g fresh weight), antioxidant activity (5.23 mg/g fresh weight), root growth and chlorophyll content are observed in the Ardabil batch when treated with G. intraradices. The lowest amounts of these values are recorded in the Fars batch where no mycorrhizal inoculation has been applied. The two batches of seeds grow into plants with different growth rates and phytochemical compositions. The growth parameters of plants improve in the case of mycorrhizal inoculation. The effects of G. intraradices are more efficient than those of G. mosseae in symbiosis with chicory plants. Based on the results of the present study and considering the compatibility of mycorrhiza with the environment, the use of these fungi can substantially increase the yield of various plants, especially medicinal plants.
Mohammad Sayyari; Monirea Moradi Farsa; Ali Azizi
Abstract
The phonological stage in which the abotic stress occurs is effective in determining the content of secondary metabolites in medicinal plants. This research deals with the effects of imposing drought stress at different phenological stages on growth and some physiological and phytochemical responses ...
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The phonological stage in which the abotic stress occurs is effective in determining the content of secondary metabolites in medicinal plants. This research deals with the effects of imposing drought stress at different phenological stages on growth and some physiological and phytochemical responses of Nepeta crispa L as a split plot design in Bu-Ali Sina University during 2019. Drought stress at three levels, including mild, moderate, and severe stress (with 80%, 60%, and 40% of field capacity, respectively) has been applied at two phenological stages (seedling establishment stage with 6 true leaves and pre-flowering stages). Results from statistical analysis show that drought stress has had a significant effect on all evaluated growth traits and phytochemical parameters of the plant including proline content, relative water content (RWC) of leaves, total phenols, flavonoids, tannins, antioxidant activities, and phenolic acids. Imposing drought stress at both phenological stages reduce plant growth, decreasing chlorophyll content and RWC. However, production of phenolic compounds and phenolic acids (including rosmarinic, chlorogenic and caffeic acids) at high level and accumulation of proline through imposing drought stress reduce the adverse effects of stress and increase the medicinal properties of the Nepeta crispa. Also, the effect of drought stress imposing at pre-flowering stage has been greater than its application in the seedling establishment stage. According to the results, it is recommended to impose moderate drought stress at pre-flowering stage to increase biosynthesis of some phytochemical compounds in Nepeta crispa medicinal plants.
Sevda Ghasemi Germi; morteza Barmaki; salim farzaneh; Mandana Amiri
Abstract
AbstractThis study aims at investigating the morpho-physiological responses of potato superelite mini-tubers to different coating treatments factorial experiment, based on randomized complete block design with three replications in Research Farm of Mohaghegh Ardabili University between 2018 and 2019. ...
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AbstractThis study aims at investigating the morpho-physiological responses of potato superelite mini-tubers to different coating treatments factorial experiment, based on randomized complete block design with three replications in Research Farm of Mohaghegh Ardabili University between 2018 and 2019. Experimental factors at four levels include mini-tubers filler (zeolite and cocopeat), superphosphate (soil and pellet application), chitosan, and superabsorbent with control treatment (without pelleting). Application of cocopeat with superphosphate leads to the highest average number of tubers per plant (8.36), fresh and dry tuber yield (18.23 and 3.88 tons per hectare), and biological yield (21.69 tons per hectare). The use of chitosan in mini-tuber pelleting decrease the activity of catalase and peroxidase enzymes (19.67% and 16.37%, respectively), increasing the activity of polyphenol oxidase (37.80%). Zeolite increase and cocopeat decrease the tuber protein percentage, compared to the control. This increase in protein percentage with increasing tuber yield leads to a 32.23% increase in tuber protein yield in zeolite application, compared to control treatment. The use of superphosphate in mini-tuber pelleting also increase the protein percentage, thus leading to a 30.30% increase in protein yield, compared to control mini-tuber. In principal component analysis (PCA), the first component, with which 35% of the changes could be explained, include the yield of protein, lysine, and methionine, and the second component, with which 25% of the changes could be explained, include the protein, methionine, lysine, and total nitrogen. Finally, the results show that the mini-tuber pelleting by superphosphate, zeolite, and superabsorbent have had significant additive effects on yield and quality traits.
jaber khordadi Varamin; Farzad Fanoodi; jafar masoud sinaki; Shahram rezvan; Ali Damavandi
Abstract
To investigate the physiological responses of sesame cultivars to the application of nano magnesium and chitosan biopolymer under different irrigation regimes, a split factorial based on randomized complete block design has been conducted with three replicates between 2017 and 2018. The irrigation cut-off, ...
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To investigate the physiological responses of sesame cultivars to the application of nano magnesium and chitosan biopolymer under different irrigation regimes, a split factorial based on randomized complete block design has been conducted with three replicates between 2017 and 2018. The irrigation cut-off, based on BBCH scale, has served as the main factor (normal irrigation, irrigation up to 50% flowering, and seed ripening), with the sub factors including Oltan and Dashtestan-2 sesame cultivars, and nano magnesium (application and non-application) and chitosan (control, 4.8, and 6.4 g.L-1). The highest mean grain yield belongs to the application of 6.4 g.L-1 chitosan under normal irrigation with an average of 1235.1 kg.ha-1. Also, the highest total chlorophyll content is observed in Dashtestan-2 genotype under normal irrigation with a mean of 24.7 mg.g-1 FW and the lowest mean have been obtained in both genotypes under irrigation up to 65 BBCH with a mean of 17.21 and 17.46 mg.g-1 FW, respectively. Application of nano fertilizer in Oltan genotype under irrigation up to 65 BBCH increases the catalase activity by 41.11%, compared to the control treatment. The highest activity of ascorbate peroxidase has been achieved when not applying chitosan under irrigation conditions up to 65 BBCH. It has risen by 55.06%, compared to the control treatment. In general, the results show that irrigation up to 65 BBCH reduces grain yield, in turn alleviated by the negative effects of stress on magnesium and chitosan nanoparticles (9.93% and 27.46%, respectively). Based on the regression analysis results, four traits, namely chlorophyll b, total chlorophyll, proline, and catalase, enter the model that explains 42.11% of the total grain yield variations. Results of simple correlation between traits and regression analysis indicate the indirect effects of physiological traits on grain yield and among the studied parameters, photosynthetic pigments has been of high account in stress conditions.
Vahid Akbarpour; Mahboobeh Ashnavar; Mohammad Ali Bahmanyar
Abstract
Plant secondary materials play an important role in health and human nutrition. According to the importance of coneflower in the production of secondary materials and also the effect of nutrient on the amount of secondary materials, a pot experiment was conducted with a completely randomized design six ...
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Plant secondary materials play an important role in health and human nutrition. According to the importance of coneflower in the production of secondary materials and also the effect of nutrient on the amount of secondary materials, a pot experiment was conducted with a completely randomized design six treatments and three replications in Sari University of Agricultural Sciences and Natural Resources in 2015. Treatments were included: chemical fertilizer (150 kg N, 120 kg P2O5 and 250 kg K2O per hectare from urea, triple super phosphate and potassium sulphate source, respectively) (CF), 30 t.ha-1 manure (M), 15 t.ha-1 M+25 percent CF, 15 t.ha-1 M+50 percent CF and 15 t.ha-1 M+75 percent CF and control (without applying chemical fertilizer and compost). Result indicated that all traits such as chlorophyll a and b, yield of anthocyanin and flavonoid in leaf and root, yield of total phenolic in flower and percentage of antioxidant activity in full bloom were affected by different fertilizer treatments. So that the maximum concentration of chlorophyll a and b (11.33 and 2.41 mg.gr-1 FW respectively) were related to the application of chemical fertilizer that with incorporation manure and chemical fertilizer (15 t.ha-1 M + 25 percent CF and 15 t.ha-1 M+75 percent CF) were statistically the same level. Meanwhile, the application of 30 t.ha-1 M had the greatest effect on anthocyanin and flavonoid in leaves and total phenolic of flower. Maximum antioxidant activity was obtained with using 15 t.ha-1 M+25 percent CF increased 10 percent and 80 percent compared to the control and CF treatments, respectively.
nastaran hemmati; azim ghasem nezhad; javad fattahi moghaddam; pouneh ebrahimi
Abstract
The present study was done to investigate the correlation between biochemical changes of grafted tree fruits and rootstock fruits in Gorgan university of agricultural science and natural resources in 2012-2013. For this purpose, the antioxidant activity and the total content of phenol and flavonoid ...
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The present study was done to investigate the correlation between biochemical changes of grafted tree fruits and rootstock fruits in Gorgan university of agricultural science and natural resources in 2012-2013. For this purpose, the antioxidant activity and the total content of phenol and flavonoid on four rootstocks such as Yuzu (Citrus junos), Shelmahalleh (Citrus sinensis var. shel mahalleh), Citrumelo (Citrus paradisi X Poncirus trifoliate), Sour orange (Citrus aurantium) and Italian and Salustiana grafted tree were studied in skin and flesh of fruits. This research was done as a factorial experiment based on completely randomized design with three replications. The result showed that the measured parameters were significantly influenced by cultivar, rootstock and tissue. The highest amount of total phenol (21.38 mg/gDM) was recorded in Italian skin on Shelmahalleh rootstock. The most antioxidant activity (85.71 percent) was produced in the skin of Citrumelo rootstock. The maximum content of total flavonoid (0.337 mg/gDM) was observed in the skin of Salustiana on Yuzu rootstock. The investigation indicated that the total phenol accumulation ability of rootstock fruit influences the antioxidant activity of the grafted tree fruits with a positive correlation Although there was significant differences between antioxidant compounds of grafted tree fruits with fruits of their rootstocks, but there was no relationship between them. It seems this was due to a combination of physiological characteristics of each group (grafted or not) of fruits.