Maryam Tatari; Seyed Asghar Mousavi
Abstract
According to problems caused by application of non-uniform seedling rootstocks in stone fruit treesorchards, the use of uniform and consistent rootstocks with these trees is essential. For determination ofthe best culture medium, explants of apical and lateral buds of Tetra, Nemaguard and GF677 rootstockswere ...
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According to problems caused by application of non-uniform seedling rootstocks in stone fruit treesorchards, the use of uniform and consistent rootstocks with these trees is essential. For determination ofthe best culture medium, explants of apical and lateral buds of Tetra, Nemaguard and GF677 rootstockswere prepared. Explants were placed in modified MS, WPM and Knop media each containing 0.6 mg/lBAP and 0.01mg/l NAA in mid-Spring and after disinfection. This research was carried out as factorialexperiment in a completely randomized design with four replicates and four observations per eachreplication. Tetra, GF677 and Nemaguard rootstocks produced the highest of plantlet numberrespectively. Nemaguard rootstock did not show any proliferation in Knop medium and Nemaguardexplants showed chlorosis on this medium. This medium caused chlorosis in produced plantlets of Tetraexplants. Explants of every three rootstocks were produced the highest plantlet number and length in MSmodified medium. Therefore, combinations of plant growth regulators were evaluated in this medium.Three different combinations were evaluated. Among different combinations of plant growth regulators(0.8 and 1 mg/l BAP and 0.1 and 0.5 mg/l NAA), the highest plantlet number and length were producedin MS medium containing 0.6mg/l BAP and 0.01mg/l NAA. Increase of plant growth regulatorsconcentrations led to vitrification and callus production in GF677 rootstock. The highest root number androoting percent were achieved in modified MS medium containing 1 mg/l NAA and explants of Tetrarootstock produced the highest root number and length.
zeinab karamad; Ebrahim Ganji Moghadam; ahmadreza bolandi
Abstract
This study was conducted with the main prupose of investigation the effects of culture medium and the concentration of growth regulators on proliferation rate, rooting and the acclimatization of Gisela 6 rootstock at Khorasan Razavi Agriculture and Natural Resource Research Center during 2011-2012. In ...
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This study was conducted with the main prupose of investigation the effects of culture medium and the concentration of growth regulators on proliferation rate, rooting and the acclimatization of Gisela 6 rootstock at Khorasan Razavi Agriculture and Natural Resource Research Center during 2011-2012. In this experiment, the proliferation and rooting were performed in six kinds of culture medium (DKW, WPM and MS in two solid and liquid media) supplemented with plant growth regulators included TDZ and BAP in zero, one, two mg/l plus 0.01 mg/l IBA in all treatments of the proliferation and IBA with zero, one, two, three mg/l in rooting step. The results showed that the highest number and length of shoot were 6.48 shoot and 3.14 cm, respectively in solid WPM medium and two mg/l BAP concentration and liquid MS medium with one mg/l concentration of TDZ. The liquid MS medium in two mg/l of IBA caused the highest percentage of rooting and root length, 93.90 percent in 11.76 cm, respectively. Although applying the liquid media resulted in better acclimatization of plantlets, but the highest percent of plantlets survival was obtained in WPM liquid medium with two mg/l concentration of IBA at about 91.80 percent.