Mohammad Yazdandoost Hamedani; Mokhtar Ghobadi; Mohammad Eghbal ghobadi; Saeid Jalali Honarmand; mohsen saeidi
Abstract
In order to evaluate the effect of foliar application of some chemicals on antioxidant enzymes activity and chlorophyll fluorescence of sunflower in different irrigation conditions as well as identifying compounds’ effectiveness in reducing the adverse effects of drought stress, this experiment ...
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In order to evaluate the effect of foliar application of some chemicals on antioxidant enzymes activity and chlorophyll fluorescence of sunflower in different irrigation conditions as well as identifying compounds’ effectiveness in reducing the adverse effects of drought stress, this experiment has been conducted at the Agricultural Research Center of Hamedan, Iran, between 2015 and 2016. Three irrigation and six foliar application treatments are evaluated in a split plot experiment based on randomized complete block design with three replications. Irrigation treatments consist of 60%, 80%, and 100% plant water requirement and foliar application treatments include abscisic acid 40 μM, Selenium 20 mg/L, Salicylic acid 500 μM, SNP 100 μM, Glycine betaine 100 mM, and the control. Results show that by decreasing the irrigation water, the activity of superoxide dismutase, peroxidase, and catalase enzymes rise sharply, while the maximum photochemical efficiency of photosystem II and grain yield decline. The interaction effect of irrigation × foliar application on the activity of antioxidant enzymes and grain yield is significant, but not so on chlorophyll fluorescence. In deficit irrigation treatments, foliar application of all chemical compounds significantly boost the activity of antioxidant enzymes, compared to the control. Application of salicylic acid raises the maximum photochemical efficiency of photosystem II and grain yield, at all irrigation conditions. Under 60%, 80%, and 100% irrigation conditions, salicylic acid application increases grain yield by 24.3%, 10.1%, and 4.9%, respectively.
Atefeh Sadat Mousavi; Masoumeh Naeemi; ali Rahemi karizaki; Abdolatif Gholizadeh
Abstract
In order to investigate the effect of chitosan and salicylic acid elicitors on osmotic adjustment and some physiological traits of Hyssop under experimental drought conditions, an experiment has been carried out as a split plot design with randomized complete block design with three replications at research ...
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In order to investigate the effect of chitosan and salicylic acid elicitors on osmotic adjustment and some physiological traits of Hyssop under experimental drought conditions, an experiment has been carried out as a split plot design with randomized complete block design with three replications at research farm of Gonbad Kavous University during 2018-2019. Irrigation factor at three levels include 7, 14, and 21 days irrigation interval as main factor and four levels of spraying including no elicitor (control treatment, spraying), salicylic acid (300 mg. l-1), Chitosan spraying (5 g. l-1), and combination of salicylic acid and chitosan, considered as auxiliary agents. Results of analysis of variance show that the interaction between spraying and irrigation has had significant effects on ion leakage, chlorophyll a content, soluble sugar, and malondialdehyde content but insignificant impacts on carotenoid and essential oil yield. Spraying of salicylic acid, with 17% reduction in ion leakage and 40% reduction in plant malondialdehyde, compared to the control, increase the stability of cell membranes under 21-day irrigation stress. In the same treatment, chitosan has increased the amount of photosynthetic pigments and total chlorophyll by 51%, compared to the control. According to the results, salicylic acid and Chitosan have been able to increase the ability of hyssop in response to water deficit stress by activating different tolerance mechanisms.
kamel sajed gollojeh; Saeid Khomari; parisa sheikhzadeh mosadegh; naser sabaghnia; mehdi mohebodini
Abstract
The present research is carried out to study the effects of foliar application of Nano material and Salicylic acid (SA) on some agronomy, yield, and yield components of spring rapeseed under different water limitation treatments. For this subject an experiment has been conducted as split plot based on ...
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The present research is carried out to study the effects of foliar application of Nano material and Salicylic acid (SA) on some agronomy, yield, and yield components of spring rapeseed under different water limitation treatments. For this subject an experiment has been conducted as split plot based on a randomized complete block design with four replications at the research fields of Faculty of Agriculture and natural resource of Mohaghegh Ardabili University, during 2017 and 2018 growing years. Water limitation treatments include full irrigation (control), irrigation termination at stem elongation, flowering and seed formation stages, and foliar spray of Nano-Material in form of Nano-silicon dioxide (nSiO2) at three levels of zero (control), 60, and 300 mg.L-1 as well as salicylic acid (SA) at three levels of zero (control), 0.5, and 2.5 mM. The results of combined analysis show that the effect of year has been significant on main branch and sub branches number per plant, number of pod per plant, number of seed per pod, and seed yield traits. Water limitation has decreased all studied treats, so the foliar application improves growth traits, yield, and yield components of rapeseed plants. The highest seed yields have been 2705 and 3271 kg.h-2, in first and second experiment years, respectively, which are from applying 300 mg.L-1 with 0.5 nSiO2 and/or 2.5 mM of SA at normal irrigation (control treatment). The lowest rate belongs to non-foliar application especially at irrigation termination at stem elongation, flowering stages.
Shahram Taheri; Ahmad Gholami; Hamid Abbasdokht; Hassan Makarian
Abstract
In order to evaluate the effects of seed priming to reduce water deficit stress in safflower cultivars, an experiment was conducted as a split plot factorial based on randomized complete block design with three replications at Shahrood Agricultural Research Center in 2015. The main plot consisted of ...
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In order to evaluate the effects of seed priming to reduce water deficit stress in safflower cultivars, an experiment was conducted as a split plot factorial based on randomized complete block design with three replications at Shahrood Agricultural Research Center in 2015. The main plot consisted of irrigation at three levels based on the evaporation from class A evaporation pan: non water deficit stress (60 mm evaporation), mild water deficit stress (120 mm evaporation) and severe water deficit stress (180 mm evaporation) and subplots consisted of two factors include safflower cultivars (Goldasht, Sina and Soffeh) and seed priming (Primed seeds with salicylic acid and non-primed). The results showed that severe water stress reduced the grain yield by about 29 percent compared to non stress conditions. In these conditions activity of superoxide dismutase, peroxidase, ascorbate peroxidase and catalase enzymes was increased by about 33, 25, 29 and 40 percent respectively. In severe water deficit conditions, content of malondialdehyde, proline and carotenoid significantly increased but the amount of chlorophyll was reduced. Priming of seeds with salicylic acid caused the antioxidant defense system activity to be increased by about 7-9 percent, therefore increased resistance of safflower plants to water stress and resulted in greater seed yield under water stress conditions. Interaction of irrigation and cultivar appeared to be significant on seed yield and activity of catalase, peroxidase and ascorbate peroxidase enzymes.
Mohammad Javad Nazarideljou; Mehran Kanani
Abstract
Regarding to a few studies on odorant compounds of cut flowers, a pot experiment was conducted to evaluate the effect of pre-harvest foliar application of salicylic acid (0, 1.5 and 2.25 mM) and a-aminooxi-b-phenyl propionic acid (0, 1.5 and 3 mM) as promoter and specific inhibitor of phenylalanine ammonia-lyase ...
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Regarding to a few studies on odorant compounds of cut flowers, a pot experiment was conducted to evaluate the effect of pre-harvest foliar application of salicylic acid (0, 1.5 and 2.25 mM) and a-aminooxi-b-phenyl propionic acid (0, 1.5 and 3 mM) as promoter and specific inhibitor of phenylalanine ammonia-lyase enzyme, respectively, on tuberose “double” basis on completely randomized design with four replications. According to the results, relative water content, cell membrane stability and vase life were significantly affected by foliar application (P<0.01). The a-aminooxi-b-phenyl propionic acid (3mM) and salicylic acid treatments (1.5 mM) increased the vase life about 68 and 34% compared with control. Post-harvest collection and analysis of tuberose floral scent was conucted using headspace system and GC-MS technique and 37 different compounds were detected. Methyl benzoate, benzyl benzoate, pentacosane and methyl salicylate were the most important odorant compounds of cut tuberose respectively. a-aminooxi-b-phenyl propionic acid treatment increased benzyl benzoate and pentacosane whereby decreased methyl benzoate and methyl salicylate amount, which were opposite to salicylic acid effect. Results showed that the Promoter and inhibitor of phenylalanine ammonia-lyase enzyme activity increased and decreased total amount of cut tuberose scent, respectively.
ABBAS MIRZAKHANI; Mansoore Jalali
Abstract
This experiment was conducted in National Institute of Ornamental Plants for extending vase life of Anturium andranum cv. Climax cut flowers. Experiment was done based on Completely Randomized Design (CRD) with three replications and three cut flowers per replicate. Treatments included Volk oil emulsion ...
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This experiment was conducted in National Institute of Ornamental Plants for extending vase life of Anturium andranum cv. Climax cut flowers. Experiment was done based on Completely Randomized Design (CRD) with three replications and three cut flowers per replicate. Treatments included Volk oil emulsion wax coating (10, 20 and 30 percent), Thyme oil (100, 150 and 250 ppm), Salicylic acid (200, 400 and 600 ppm) and Aloe vera gel as wax coating (2.5, 5 and 7.5 percent). Sucrose (5 percent) was added to all of the treatments. After treatments some quantitative and qualitative traits were measured. Results showed that vase life of cut flowers increased significantly in volk oil wax coating 10 percent (27.33 days) than control (17.88 days), the lowest vase life was found with Thyme oil treatment. The flowers which treated with 2.5 percent of Aloe vera gel had the highest solution uptake than control. Treatment with thyme oil of 150 ppm caused the highest ion leakage (69.5 percent) compared to the others. The lowest ion leakage (10.71 percent) was obtained in Aloe vera gel treatment of 7.5 percent.
Atena Tanoori; Azim Ghasemnezhad; Mahdi Alizadeh
Abstract
Modern biotechnology and genetic engineering using techniques and special laboratory tools plays an important role in the pharmaceutical industry and medicinal plant propagation. Among these techniques, it can be noted that a wide range of elicitors are widely used for inducing metabolites ...
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Modern biotechnology and genetic engineering using techniques and special laboratory tools plays an important role in the pharmaceutical industry and medicinal plant propagation. Among these techniques, it can be noted that a wide range of elicitors are widely used for inducing metabolites at invitro culture. Due to the pharmaceutical properties of artichoke and its history, it has been deeply noticed by pharmaceutical industry and its cultivation is expanding rapidly. In present study the effect of two elicitors' methyl jasmonate (MJ) and salicylic acid (SA) on the callus mass growth and survival of invitro culture was investigated. Callus cultures of artichoke were established by transferring seedling on solidified MS medium supplemented with different concentrations of salicylic acid and methyl jasmonate. Four weeks after subculture, the callus was collected and weighed. Results showed that the SA supplementation reduced fresh weight of callus. Contrary to fresh weight, a direct increase was observed in dray weight by increasing the level of SA. Opposite to SA different concentration of MJ not only did not changed the fresh weight, but also reduced the dry weight of callus. Study the pigment changes under different treatments of SA and MJ showed that, contrary to cartonoid a direct reduction was observed in chlorophyll content by increasing in both SA and MJ concentration. Finally it can be suggested that, however both SA and MJ as most important elicitors increase secondary metabolite production of callus, their availability in high concentration affect the cell grow activity, callus survival and the biomass production.
Mehdi Abdolahi; Farid Shekari
Abstract
To evaluate the effects of seed priming with salicylic acid on quality and baking traits of wheat flour, cv. Alvand, under different sowing dates, a factorial experiment was conducted on the basis of complete randomized block design. The factors were seed priming with salicylic acid (including untreated ...
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To evaluate the effects of seed priming with salicylic acid on quality and baking traits of wheat flour, cv. Alvand, under different sowing dates, a factorial experiment was conducted on the basis of complete randomized block design. The factors were seed priming with salicylic acid (including untreated seeds or control treatment, 400, 800, 1200, 1600, 2000 and 2400 µM concentrations of salicylic acid) and two sowing dates (23rd of Oct. and 22nd of Nov.). Results showed that priming slightly decreased (less than 1%) protein percent compared to control treatment in both sowing dates. It may be due to positive effects of salicylic acid on seed yield and higher accumulation of starch in seeds compared to protein. On the other hand, priming enhanced Zeleny number and loaf volume. Wet gluten, gluten index and seed yield increased by application of seed priming. This was higher in second sowing date compare to the first sowing date. In addition, higher concentrations of salicylic acid lead to more increment in measured parameters.
Hossein Afshari; Raheleh Zahedi; Tahereh Parvaneh; Masoud Zadehbagheri
Abstract
In order to study the effect of different concentrations of salicylic acid in two commercial apricotcultivars of Shahrood namely ‘Shahrood 41’ and ‘Jaffari’, under cold stress, a factorial experiment wasconducted in a randomized complete block design with three replications in ...
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In order to study the effect of different concentrations of salicylic acid in two commercial apricotcultivars of Shahrood namely ‘Shahrood 41’ and ‘Jaffari’, under cold stress, a factorial experiment wasconducted in a randomized complete block design with three replications in 2012 at the AgriculturalResearch Center of Shahrood city. The test had four factors including: SA with three levels (0.625, 0.125,0.25mM), three stages of phenological growth (dormancy, swelling and flowering) and four temperature(four, zero, -2 and -4 degree centigrade) and two apricot cultivars (‘Shahrood 41’ and ‘Jaffari’) andmeasured traits were proline and soluble sugars. ‘Jaffari’ cultivar had the highest amount of solublesugars at -4 degree centigrade in bud swelling stage and the maximum rate of proline belonged to0.25mM of SA in the same cultivar, -4 degree centigrade and bud swelling stage. Ion leakage at differenttemperatures and different phonological growth stages were significantly different at five percent level.‘Jaffari’ with lower ion leakage at -4 degree and higher proline content than ‘Shahrood 41’ had higherresistance to frost.
Majedeh Malekian; Khodayar Hemmati; Azim Ghasemnezhad; Mohammad Barzali
Abstract
German chamomile (Matricaria chamomilla) is one of the most important industrial medicinal plant. Inthis experiment the effects of foliar application of salicylic acid in different levels (0, 10-2, 10-4, 10-6) wereinvestigated on the floral traits and essential oil content of three ecotypes (Italian, ...
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German chamomile (Matricaria chamomilla) is one of the most important industrial medicinal plant. Inthis experiment the effects of foliar application of salicylic acid in different levels (0, 10-2, 10-4, 10-6) wereinvestigated on the floral traits and essential oil content of three ecotypes (Italian, Tehran, Zabol) ofGerman chamomile. The results showed that maximum percentage of essential oil (1.5 weight percent)was obtained from Zabol ecotype as sprayed with 10-4 M of salicylic acid and Maximum oil yield (0.085gr per pot) was obtained from Italy ecotype in which treated with 10-2 M salicylic acid. Among theidentified sesquiterpenes of essential oil, Chamazulene, E-β Farencene and α-Bisabolol oxide A weresignificantly varied based on the experimental treatments. The highest amount of Chamazulene (3.789percent) was recorded in Italian ecotype in which treated with 10-2 M salicylic acid, and the same ecotypeproduced highest amount of farencene (2.264 percent) when treated with 10-2 M Salicylic acid. Theapplication of salicylic acid in concentrations of 10-4 and 10-6 M increased the α-Bisabolol oxide A(respectively, 70.907 and 73.482 percent) Compare with control. Generally, contrary to essential oil yieldcomponent, only the essential oil yield and its constituent were affected by salicylic acid application andthe responses of experimental ecotypes was varied.
Fatemeh Salarpour Ghoraba; Hassan Farahbakhsh
Abstract
Recently, application of SA has increased to improve plants resistance to stresses such as drought. Hence, to investigate SA effects on morphophysiological characteristics of Fennel(Foeniculum vulgare Mill.) under drought stress, a split plot experiment based on latin square design with three replications ...
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Recently, application of SA has increased to improve plants resistance to stresses such as drought. Hence, to investigate SA effects on morphophysiological characteristics of Fennel(Foeniculum vulgare Mill.) under drought stress, a split plot experiment based on latin square design with three replications was carried out at research farm of Shahid Bahonar University of Kerman in 2012. Three levels of drought stress (50, 75 and 100% of field capacity) and 3 concentrations of SA (0, 0.5 and 1mM) were considered as main and sub plot respectively. Foliar application of SA was performed at 3 to 4 leaf growth stage and before application of drought stress. The results showed that drought stress reduced plant height, the number and length of internodes on the main stem , grain yield and leaf protein (14.2, 2.4, 31.5, 51 and 23.5 percent respectively) and increased lipid peroxidation, hydrogen peroxide and phenolic compounds(66.6, 10.5 and 14.1 percent) compared to control. With increasing concentrations of salicylic acid, except for a reduction in lipid peroxidation and hydrogen peroxide (31.8 and 13.7 percent) a significant increase was observed in other traits when compared to control. Interaction of drought stress and SA on internode length on the main stem, lipid peroxidation and protein were signified. it was concluded that salicylic acid whit concentration of 1 mM has an effective role in alevieting stress injuries induced by drought.
sayed ali tabatabaei
Abstract
One of the effects of reducing water content on soil is reduction of growth and development of seedlings and variation of field development of them. It was found that seed priming improves some characteristics of seedling and plant. An experiment was conducted for evaluating the effect of seed priming ...
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One of the effects of reducing water content on soil is reduction of growth and development of seedlings and variation of field development of them. It was found that seed priming improves some characteristics of seedling and plant. An experiment was conducted for evaluating the effect of seed priming by salicylic acid on some characteristics of barley seed at early growth stages, antioxidant enzyes activity and proline content under drought stress. The first factor was priming with salicylic acid (zero, 50, 100 and 150 ppm) and the second factor was three levels of drought (zero, -6 and -12 bar). The results showed that SA pretreatment effect on germination percentage, germination rate, dry weight, protein, catalase, ascorbat peroxidase activity and proline content were significant. The interaction effect of stress seed treatment on leaf number was not significant but the main factors were significant. The highest germination percentage (98.7), germination rate and seedling dry weight were found at 100 ppm concentrations of salicylic acid. Under drought stress conditions, protein content decreased but salicylic acid pretreatment increased the valuet. Also, drought stress conditions increased enzyme activity and proline content, but salicylic acid pretreatment decreased them.
Maryam Hadian-Deljou; Hasan Sarikhani
Abstract
Fruit browning and tissue softening due to damages during handling, quality losses and water loss are the major limiting factors reducing postharvest life of early ripening apples. In this study, the effect of salicylic acid (SA) on fruit quality of apple cv ‘Golabe-Kohanz’ during postharvest ...
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Fruit browning and tissue softening due to damages during handling, quality losses and water loss are the major limiting factors reducing postharvest life of early ripening apples. In this study, the effect of salicylic acid (SA) on fruit quality of apple cv ‘Golabe-Kohanz’ during postharvest life were investigated. Harvested fruits were immersed in zero, one, two and four mM SA and were maintained for 43 days at zero ?C. SA decreased the soluble solids content at last stages of storage. In addition, SA reduced softening rate and water loss. At the end of storage, the highest firmness was observed in one and two mM SA treated fruits and the highest water loss was measured in control. Treatment of fruits by all three concentrations of SA showed a little browning. The highest browning was observed in those of control. Also SA delayed reduction of the total phenolic. The results demonstrate that SA postharvest treatment of apple cv. ،Golabe-Kohanz, through reducing browning and maintaining fruit quality increased postharvest life.