Kosar Asadzadeh; Habiballah Nadian Ghomsheh; Vahid Keshavarz-Tohid; Abdolreza Siahpoosh
Abstract
Objective: In order to investigate the impact of plant growth-promoting Pseudomonads isolates and filter cake on the alterations of chlorophyll pigments and growth components in spinach plant under metribuzin herbicide stress, a factorial experiment was conducted as completely randomized blocks at the ...
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Objective: In order to investigate the impact of plant growth-promoting Pseudomonads isolates and filter cake on the alterations of chlorophyll pigments and growth components in spinach plant under metribuzin herbicide stress, a factorial experiment was conducted as completely randomized blocks at the greenhouse of the Department of Soil Science of the Agricultural Sciences and the Natural Resources University of Khuzestan in 2018-2019.
Methods: In this study, Pseudomonas alloputida RUM14 and P. protegens CHA0 were used as PGPR isolates and filter cake was utilized as organic fertilizer.
Results: Statistical analysis of the study’s results indicated that treating spinach plants with Pseudomonas alloputida RUM14, P. protegens CHA0, and filter cake significantly increases chlorophyll a (47, 21, 29%, respectively) and b pigments (42, 20, 18%, respectviely) and carotenoids (96, 33 and 84%) and also increases the dry weight of root (respectively 59, 30, 45%), length of root (108, 51, 53%), dry weight of stem (56, 49, 13%) and the stem length (31, 19, 14%). Statistical analysis clarified that applying metribuzin herbicide at the rate of 100 grams per hectare dramatically reduces the content of photosynthetic pigments and growth components. However, utilization of growth-promoting rhizobacteria and filter cake effectively decrease the extent of such damage.
Conclusion: The results of this investigation clarified for the first time that the use of Pseudomonads plant growth-promoting rhizobacteria and/or filer cake, significantly enhance the photosynthetic pigments and growth components of spinach plant. Additionally, the mentioned treatments reduce the damage caused by metribuzin herbicide application.
Raouf Seyed sharif; reza seyed sharifi; Hamed Narimani
Abstract
In order to study the effect of methanol and seed inoculation with plant growth promoting rhizobacteria on grain filling components, chlorophyll content and grain yield of safflower (Carthamus tinctorius L.) under various irrigation levels, a factorial experiment has been conducted based on randomized ...
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In order to study the effect of methanol and seed inoculation with plant growth promoting rhizobacteria on grain filling components, chlorophyll content and grain yield of safflower (Carthamus tinctorius L.) under various irrigation levels, a factorial experiment has been conducted based on randomized complete block design with three replications at the research farm of University of Mohaghegh Ardabili in 2020. The experimental factors include irrigation (full irrigation as control, irrigation withholding at 50% heading-bud, and flowering stages), biofertilizers application (no application as control, seed inoculation with Flavobacterium, Pseudomonas and both application of Flavobacterium and Pseudomonas), and methanol foliar application: (foliar application with water as control and foliar application with 10% and 20% volume of methanol). The results show that the content chlorophyll a (41.57%), b (74.59%) and total chlorophyll (49.33%), grain filling period, and effective grain filling period (27.32% and 72.89% respectively) and grain yield (69.2%) have increased under full irrigation condition, foliar application 20% volume of methanol, and both application of Flavobacterium with Pseudomonas in comparison with irrigation withholding at 50% heading-bud and no application of biofertilizers and methanol. Generally, the results show that application of plant growth-promoting bacteria and methanol can increase grain yield of safflower by improving chlorophyll content and grain filling components under water limitation condition.
Fatemeh Zamani; Reza Amirnia; Esmaeil Rezaei-chiyaneh; Amir Rahimi
Abstract
In order to investigate the effect of biofertilizers on the seed yield and chemical composition of essential oil of fennel (Foeniculum vulgare L.), a field experiment was conducted as factorial based on randomized complete block design with three replications and twelve treatments at the Research Farm ...
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In order to investigate the effect of biofertilizers on the seed yield and chemical composition of essential oil of fennel (Foeniculum vulgare L.), a field experiment was conducted as factorial based on randomized complete block design with three replications and twelve treatments at the Research Farm of the Faculty of Agriculture, University of urmia, Iran during growing season of 2015-2016. The first factor included of three Landraces (Urmia, Hamdan and Germany) and second factor included of four biofertilizers as: complete biofertilizers (consumption of nitrogen, phosphorus, potassium and sulfur supplier bacteria), mycorrhizal fungi (Glomus intraradices), integrated Bacterial biofertilizers with mycorrhizal fungi and control treatment. Results indicated that application of biofertilizers enhanced the seed yield, colonization percentage, nutrients absorption, essential oil content, essential oil yield and essential oil composition. Among treatments, combined usage of biofertilizers showed that great increasing in studied traits than individual consumption. Also, The effect of landrace on all studied quantitative and qualitative traits was significant except for seed nitrogen. Seed yield of the German landrace were higher than Hamedan and Urmia landrace. The main components of the essential oils fennel were; Anethol, Fenchone, Limonene and p-Allylanisole. The highest amount of anethole was found in Urmia landrace in inoculated with mycorrhiza and bacterial biofertilizer. In general, the results showed that the use of biofertilizers had a significant effect on the improvement of quality and quantity of fennel traits.
Azam Feli; Saeideh Maleki Farahani; Hossein Besharati
Abstract
In order to evaluate the effects of organic, chemical and biological fertilizers on plant and soil properties in saffron cultivation the experiment was performed as factorial with two factors based on a randomized complete block design with three replications in the research farm of Shahed University ...
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In order to evaluate the effects of organic, chemical and biological fertilizers on plant and soil properties in saffron cultivation the experiment was performed as factorial with two factors based on a randomized complete block design with three replications in the research farm of Shahed University during 2015-2016 growing seson. in this experiment the first factor was nitrogen fertilizer (urea) in three levels (0, 50 and 100 kg per ha) and second factor was different types of non-chemical fertilizers in four levels (control, vermicompost, PGPR biofertilizer containing bacteria Pseudomonas and Bacillus and integrated application of biofertilizer and vermicompost). The results showed that fertilizer treatments affect significantly on qualitative and quantitative properties of plant and soil. Among the fertilizer treatments, the integrated application of vermicompost alongwith 50 kg/ha chamical fertilizers showed the most significant increment in yields and improved soil properties. As in this treatment stigma dry yield, soil nitrogen content, soil organic mater and available soil phosphorus increase by 42.6, 66.67, 68.39, and 43.75% compared to control, respectively. Totally integrated application of vermicompost alongwith half of recommended urea fertilizer can increase yield and improve soil properties in saffron cultivation.
Farshid Hassani; Ahmad Asgharzadeh; Mohammadreza Ardakani; Aidin Hamidi
Abstract
This research was conducted to study the effect of PGPR (Plant Growth Promoting Rhizobacteria) on mini-tuber yield and absorption of nutrients in the greenhouse ofSeed and Plant Certification and Registration Research Institute in 2012. Minituber derived from tissue culture of two potato cultivars (‘Agria’ ...
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This research was conducted to study the effect of PGPR (Plant Growth Promoting Rhizobacteria) on mini-tuber yield and absorption of nutrients in the greenhouse ofSeed and Plant Certification and Registration Research Institute in 2012. Minituber derived from tissue culture of two potato cultivars (‘Agria’ and ‘Sante’) which were inoculated with 116 and 173 strains of Pseudomonas and two species of Bacillus (B. megaterium and B. Subtilis) all along with control treatments as separately and in mixture with each other. A pot experiment was conducted using factorial based on randomized complete design with four replications. After tuber-filling stage nutrient elements percentage was assayed. After harvesting, minitubers were separated and total yield in standard size was determined. Inoculation of PGPRs had a significant effect on yield and nutrient elements. The rate of absorption of nutrients and minituber production of two varieties showed a significant difference. Mean Comparison of traits showed that dual inoculation of two types of bacterial treatments had the most influence on the absorption of nutrients as well as the number minituber in standard size compared to the control (non-inoculated).‘Sante’ cultivarproduced the highest number of minituber compared with cv. ‘Agria’.According to the results, dual and separate inoculation of potatoes with PGPR not only intensify the synergy effects through growth and developments of roots, but also increase the nutrients absorption which cause enhancement of the tuberization and yield.