Maryam Tatari; Seyed Asghar Mousavi
Abstract
According to problems caused by application of non-uniform seedling rootstocks in stone fruit treesorchards, the use of uniform and consistent rootstocks with these trees is essential. For determination ofthe best culture medium, explants of apical and lateral buds of Tetra, Nemaguard and GF677 rootstockswere ...
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According to problems caused by application of non-uniform seedling rootstocks in stone fruit treesorchards, the use of uniform and consistent rootstocks with these trees is essential. For determination ofthe best culture medium, explants of apical and lateral buds of Tetra, Nemaguard and GF677 rootstockswere prepared. Explants were placed in modified MS, WPM and Knop media each containing 0.6 mg/lBAP and 0.01mg/l NAA in mid-Spring and after disinfection. This research was carried out as factorialexperiment in a completely randomized design with four replicates and four observations per eachreplication. Tetra, GF677 and Nemaguard rootstocks produced the highest of plantlet numberrespectively. Nemaguard rootstock did not show any proliferation in Knop medium and Nemaguardexplants showed chlorosis on this medium. This medium caused chlorosis in produced plantlets of Tetraexplants. Explants of every three rootstocks were produced the highest plantlet number and length in MSmodified medium. Therefore, combinations of plant growth regulators were evaluated in this medium.Three different combinations were evaluated. Among different combinations of plant growth regulators(0.8 and 1 mg/l BAP and 0.1 and 0.5 mg/l NAA), the highest plantlet number and length were producedin MS medium containing 0.6mg/l BAP and 0.01mg/l NAA. Increase of plant growth regulatorsconcentrations led to vitrification and callus production in GF677 rootstock. The highest root number androoting percent were achieved in modified MS medium containing 1 mg/l NAA and explants of Tetrarootstock produced the highest root number and length.