Esmaeil Gholinezhad; Bakhtiar Lalehgani; Shahryar Kazemi
Abstract
In order to investigate the effect of polyamines on antioxidant activity and biochemical traits of Camelina under salinity stress conditions, a pot (as poen air) experiment was conducted at Payame Noor University (PNU), Iran in March 2022 as factorial layout based on a completely randomized design with ...
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In order to investigate the effect of polyamines on antioxidant activity and biochemical traits of Camelina under salinity stress conditions, a pot (as poen air) experiment was conducted at Payame Noor University (PNU), Iran in March 2022 as factorial layout based on a completely randomized design with 12 treatments and 3 repetitions. The salinity treatment was at three salinity levels (0, 15 and 30 dS/m) and 4 foliar spraying levels including 1- spermine (2 mM), 2- spermidine (2 mM), 3- putrescine (2 mM) and control (pure water foliar spraying). The results showed that with the increasing salinity stress, oil and protein contents and dry seed weight, chlorophyll index, shoot organic and mineral percentage decreased, but protein percentage, shoot ash percentage, soluble sugars, antioxidant activity and ion leakage percentage increased significantly. Salinity stress of 30 and 15 ds/m compared to the control, reduced the chlorophyll index (32 and 15 percent) and mineral percentage (19 and 3 percent), oil percentage (17 and 9 percent), dry oil weight in pot (62 and 21 percent) and dry seed weight (52 and 10 percent), but protein percentage (9 and 3 percent), ash percentage (33 and 30 percent), soluble sugars (32 and 20 percent) and antioxidant activity (21 and 13 percent) increased, respectively. Also, foliar spraying with polyamines increased the above traits, but decreased the percentage of ion leakage. Therefore, in order to decreasing the effects of salinity stress and increasing the dry seed and oil weights in pot, spraying with polyamines, especially spermine, is suggested.
Raouf Seyed sharif; reza seyed sharifi
Abstract
In order to study the effects of nitrogen starter, putrescine, and nano silicon on yield and some physiological and biochemical traits of rainfed chickpea (Cicer arietinum L.), an experiment was carried out as factorial based on randomized complete block design with three replications in a farm near ...
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In order to study the effects of nitrogen starter, putrescine, and nano silicon on yield and some physiological and biochemical traits of rainfed chickpea (Cicer arietinum L.), an experiment was carried out as factorial based on randomized complete block design with three replications in a farm near Ardabil in 2021. The experiment factors include nitrogen starter (no-nitrogen starter, application of 20 and 40 kg.ha-1 by urea), putrescine foliar aplication (foliar application with water, application of 0.5 and 1 mM putrescine), and nano silicon (foliar application with water, application of 25 and 50 mg.L-1). Means comparision show that the highest level of nano silicon, putrescine, and nitrogen starter have increased chlorophyll a, chlorophyll b, total chlorophyll, and proline content (139%, 137%, 138%, and 80%, respectively), compared to no application of nano silicon, putrescine, and starter nitrogen. The highest level nano silicon and putrescine has increased the activity of catalase, peroxidase enzymes and grain yield (40%, 16.3%, and 16.6%, respectively), compared to no application of nano silicon and putrescine. Maximum quantum yield of photosystem II (7.4%, 8%, and 15.5%, respectively) and the activity of polyphenol oxidase enzyme (17%, 9%, and 21%, respectively) are obtained at the highest level starter nitrogen, putrescine, and nano silicon. It seems that the application of nano silicon, putrescine, and starter nitrogen can increase grain yield of chickpea under rainfed condition as it improves physiological and biochemical traits.
Somayeh Ehsanfar; Ali Sorooshzadeh; Seyed Ali Mohammad Modarres-Sanavy; Majid Ghorbani Javid
Abstract
To investigate the effect of polyamines on vegetative and biochemical traits of Crocus sativus L., an experiment was conducted as factorial in a randomized complete block design with three replications in 2015-2016 in Training Field of Tarbiat Modares University with three sizes of corms (3-5, 5-7 and ...
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To investigate the effect of polyamines on vegetative and biochemical traits of Crocus sativus L., an experiment was conducted as factorial in a randomized complete block design with three replications in 2015-2016 in Training Field of Tarbiat Modares University with three sizes of corms (3-5, 5-7 and 7-10 g) and five concentrations of polyamines (zero in control, zero in distilled water, spermidine 0.5 and 1 , putrescine 0.5 and 1 ) as the treatments. Results showed that plants treated with putrescine 0.5 and spermidine 1 mM had the highest germination percentage (96.56 percent) and the highest number of lateral sprouts (average number of 8.33), respectively. The length and weight of the leaves were negatively affected by polyamine treatment. The highest chlorophyll content and corm yield were observed in plants treated with Putrescine 0.5 mM and 1 mM, respectively. The content of starch and soluble carbohydrates in dormant corms at the end of growing season were not affected by polyamines significantly; however, total phenolic and flavonoid content in these corms were respectively decreased and increased as a result of applying polyamines. Having no significant effect on stigma dry weight and picrocrocin content, polyamine treatment increased the number of flowers and crocin content in small corms. It is concluded that to obtain better vegetative and biochemical traits of saffron, corms more than 7 g, treated with putrescine, should be used.
Ali Akbari; Orang Khademi; Yavar Sharafi; Seyed Jalal Tabatabaei
Abstract
Strawberry is sensitive to salinity stress. This experiment was conducted as factorial based on RCBD design to investigate the effects of putrescine treatments on reducing harmful effects of NaCl salinity stress in strawberry at Shahed University in 2015. NaCl treatments, 0, 25 and 50 mM, were applied ...
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Strawberry is sensitive to salinity stress. This experiment was conducted as factorial based on RCBD design to investigate the effects of putrescine treatments on reducing harmful effects of NaCl salinity stress in strawberry at Shahed University in 2015. NaCl treatments, 0, 25 and 50 mM, were applied in hydroponic medium on from the root zone of ‘Camarosa’ cultivar. Putrescine treatments were also applied at 0, 1.5 and 3 mM as foliage spray during growth period in 4 times with 15 days intervals. The results showed that, NaCl treatment especially at 50 mM, reduced shoot length, shoot fresh and dry weight, root fresh and dry weight, leaf number, leaf chlorophyll content, fruit number, fruit fresh and dry weight, yield and titratable acidity, and increased anthocyanin and total soluble solid content. Salinity stress had no effects on the root length. Application of 1.5 and 3 mM putrescines reduced harmful effects of salinity stress. Putrescines increased almost all characteristics under salinity condition and reduced total soluble solid content. Therefore, according to the results, putrescine, in both levels of 1.5 and 3 mM, could be a well strategy to reduce the limitation of salinity stress in strawberry production.
Fereshteh Kamiab
Abstract
In this research, the effect of putrescine, spermine and spermidine on the vase life of cut red carnation (Red Corsa) and some physiological traits was investigated in horticultural laboratory of Azad University branch Rafsanjan in 2015. Therefore, an experiment based on completely randomized design ...
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In this research, the effect of putrescine, spermine and spermidine on the vase life of cut red carnation (Red Corsa) and some physiological traits was investigated in horticultural laboratory of Azad University branch Rafsanjan in 2015. Therefore, an experiment based on completely randomized design with tree levels of 1, 2 and 3mM of putrescine and 0.5, 1 and 2mM of spermidine and 1, 2 and 3mM of spermine with 3 replications was performed. The recorded traits included vase life and morphological and physiological factors. The results indicated that all of the treatments increased vase life significantly compared to control treatment. On the other hand polyamines decreased ethylene production. Spermidineof 2 mM caused the highest vase life and the lowest ethylene production. All of the treatments especially 2 mM of Spermidine increased flower diameter and solution uptake content significantly compared to control treatment and 2 Mm of spermidine have been the most effective treatment. 1 and 2 mM of spermidine were only treatments that don’t reduce dry matter percentage significantly in the flowers. 2 mM of Spermidine caused the maximum amount of physiological traits as protein, chlorophyll and total soluble solids in the leaves of flowers. Therefore, 2 mM of Spermidine is suggested as the best treatment to increase postharvest life in Carnation cv. Red Corsa.
seyedeh samaneh hosseini; Noorollah Ahmadi; Abbas Yadollahi
Abstract
This research was conducted to determine the optimum bud or flower developing stage for pollen collection and a proper pollen germination medium and to evaluate the effect of storage temperature and different concentrations of putrescine on pollen viability and germination of two genotypes of damask ...
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This research was conducted to determine the optimum bud or flower developing stage for pollen collection and a proper pollen germination medium and to evaluate the effect of storage temperature and different concentrations of putrescine on pollen viability and germination of two genotypes of damask rose (‘Azaran’ and ‘Kashan’). The main experiment was performed based on a factorial design, with 16 treatment and 4 replications. Factors studied included four levels of putrescine (concentrations zero, 0.25, 0.5 and 2.5 mM) and four levels of pollen storage temperatures (25, four, -20 and -80°C). The best stage for collecting pollen grain was semi-open bud stage and the optimum medium containing 300 μg/l CaNO3, 200 μg/l MgSO4, 100 μg/l KNO3, 100 μg/l H3BO3, 15 percent sucrose and 1.2 percent agar. In ‘Azaran’ genotype, maximum and minimum pollen germination rate was obtained in media containing 0.25 and 2.5 mM putrescine, respectively. Storage condition of pollen grains resulted in decreasing the pollen germination rate in ‘Kashan’ genotype, while storage of pollen grains at -80°C revealed higher germination rate on medium containing 0.25 mM putrescine. As a result, maximum pollen germination percentage was obtained by storage of pollens in low temperature (-80°C) and in media containing low concentrations of putrescine.