Hossein Afshari; Raheleh Zahedi; Tahereh Parvaneh; Masoud Zadehbagheri
Abstract
In order to study the effect of different concentrations of salicylic acid in two commercial apricotcultivars of Shahrood namely ‘Shahrood 41’ and ‘Jaffari’, under cold stress, a factorial experiment wasconducted in a randomized complete block design with three replications in ...
Read More
In order to study the effect of different concentrations of salicylic acid in two commercial apricotcultivars of Shahrood namely ‘Shahrood 41’ and ‘Jaffari’, under cold stress, a factorial experiment wasconducted in a randomized complete block design with three replications in 2012 at the AgriculturalResearch Center of Shahrood city. The test had four factors including: SA with three levels (0.625, 0.125,0.25mM), three stages of phenological growth (dormancy, swelling and flowering) and four temperature(four, zero, -2 and -4 degree centigrade) and two apricot cultivars (‘Shahrood 41’ and ‘Jaffari’) andmeasured traits were proline and soluble sugars. ‘Jaffari’ cultivar had the highest amount of solublesugars at -4 degree centigrade in bud swelling stage and the maximum rate of proline belonged to0.25mM of SA in the same cultivar, -4 degree centigrade and bud swelling stage. Ion leakage at differenttemperatures and different phonological growth stages were significantly different at five percent level.‘Jaffari’ with lower ion leakage at -4 degree and higher proline content than ‘Shahrood 41’ had higherresistance to frost.
Mehri Mashayekhi; Fariborz Habibi; Mohamad Amiri
Abstract
Mechanisms of drought stress tolerance of GF677 rootstock, peach and almond hybrid, (Prunus persica × Prunus amygdalus) were studied under in vitro conditions. Plantlets of GF677 rootstock were subcultured into the Murashige and Skoog (MS) solid proliferation medium containing 1 mg/l BA (6-Benzyladenine) ...
Read More
Mechanisms of drought stress tolerance of GF677 rootstock, peach and almond hybrid, (Prunus persica × Prunus amygdalus) were studied under in vitro conditions. Plantlets of GF677 rootstock were subcultured into the Murashige and Skoog (MS) solid proliferation medium containing 1 mg/l BA (6-Benzyladenine) and 0.1 mg/l NAA (naphthaline acetic acid) in four drought stress levels 0 (control), 10, 20 and 30 g/l polyethylene glycol 6000 (corresponding to osmotic potentials 0, -0.2, -0.4 and -0.6 Mpa, respectively). After six weeks results showed that induced drought stress had a significant effect on measured parameters. By increasing drought levels in the culture medium, antioxidant enzymes activity (catalase and peroxidase), total protein content and proline content significantly increased; meanwhile soluble sugars had non-significant increasing in the different levels of drought. According to the results it can be concluded that, the most important mechanisms of drought tolerance of GF677 rootstock under in vitro conditions are the use of antioxidant defense system, increasing protein synthesis (enhancing genes expression) and proline accumulation and osmoregulation by soluble sugars had less important.
sayed ali tabatabaei
Abstract
One of the effects of reducing water content on soil is reduction of growth and development of seedlings and variation of field development of them. It was found that seed priming improves some characteristics of seedling and plant. An experiment was conducted for evaluating the effect of seed priming ...
Read More
One of the effects of reducing water content on soil is reduction of growth and development of seedlings and variation of field development of them. It was found that seed priming improves some characteristics of seedling and plant. An experiment was conducted for evaluating the effect of seed priming by salicylic acid on some characteristics of barley seed at early growth stages, antioxidant enzyes activity and proline content under drought stress. The first factor was priming with salicylic acid (zero, 50, 100 and 150 ppm) and the second factor was three levels of drought (zero, -6 and -12 bar). The results showed that SA pretreatment effect on germination percentage, germination rate, dry weight, protein, catalase, ascorbat peroxidase activity and proline content were significant. The interaction effect of stress seed treatment on leaf number was not significant but the main factors were significant. The highest germination percentage (98.7), germination rate and seedling dry weight were found at 100 ppm concentrations of salicylic acid. Under drought stress conditions, protein content decreased but salicylic acid pretreatment increased the valuet. Also, drought stress conditions increased enzyme activity and proline content, but salicylic acid pretreatment decreased them.
Fariborz Habibi; Mohammad Esmaeil Amiri
Abstract
Enzymaticactivity and biochemical responsesof two citrus rootstocks [sour orange (Citrus aurantium L.) and trifoliate orange (Poncirus trifoliata Raf.)] to in vitro salinitystress were studied. Explants of both rootstocks were transferred to Murashige and Skoog (MS) solid proliferation medium containing ...
Read More
Enzymaticactivity and biochemical responsesof two citrus rootstocks [sour orange (Citrus aurantium L.) and trifoliate orange (Poncirus trifoliata Raf.)] to in vitro salinitystress were studied. Explants of both rootstocks were transferred to Murashige and Skoog (MS) solid proliferation medium containing 8.9 µM Benzyladenine (BA) and 0.5 µM Naphthaline Acetic Acid (NAA) supplemented with 0, 50, 100, 150, 200 mM of sodium chloride (NaCl) with six replicates. After six weeks, results showed that, rootstock, salinity levels and their interaction, had a significant effect on all of the measured parameters. Antioxidant enzymes activity such as: catalase, peroxidase, ascorbate peroxidase, proline content, soluble sugars and malondialdehyde (MDA) content increased in both rootstocks by increasing salinity level. The amounts of increasing in sour orange rootstock were greater than trifoliate orange, except for peroxidase enzyme and MDA. Total protein content decreased by increasing salinity level in both rootstocks. Sour orange rootstock has higher total protein content than trifoliate orange. According to the results, sour orange rootstock was more tolerant to salt stress.
Nafiseh Asadi nasab; Payman Hassibi; Habibollah Roshanfekr; Moosa Meskarbashi
Abstract
To study some physiological and morphological traits of sugar beet varieties in response to salinity stress, three multi-germ sugar beets (13030, 22393 and IC) were grown in the greenhouse, Faculty of Agriculture, Shahid Chamran University, in 2009. The experiment was carried out under three levels of ...
Read More
To study some physiological and morphological traits of sugar beet varieties in response to salinity stress, three multi-germ sugar beets (13030, 22393 and IC) were grown in the greenhouse, Faculty of Agriculture, Shahid Chamran University, in 2009. The experiment was carried out under three levels of salinity in from of sodium chloride source, that included control (zero), 100 and 200 mM uses factorial design test based on a randomized complete block with three replications. Seeds were sown in plastic pots and salt treatments were begun 30 days after sowing. After 60 days salt treatment, the plants were harvested and analyzed. The analysis of variance results showed that salinity had significant effect on all of the parameters. The results of mean comparisons showed that by increasing salinity, root dry weight, shoot and leaf area significantly decreased, but salinity had not significant effect on the leaf number. Indeed, by increasing of salinity, the relative water content of leaf and stomatal conductance were decreased, while, the relative membrane permeability and proline content in leaves of all genotypes increased. Higher concentrations of sodium chloride decreased osmotic potential. An increase of proline neither affected electrolyte leakage nor plant water status. Therefore, in sugar beet, the antioxidant role of proline could not provide plasma membranes protection against damage caused by salt stress. According to this study, root dry weight of 90 days plants, sis was related to drought tolerance in the three varieties studied.
Zahra Mehrabi zadeh; Parviz Ehsan Zade
Volume 13, Issue 2 , March 2012, , Pages 75-88
Abstract
Sesame could be an appropriate oilseed crop for water limited environments. This research was aimed at studying growth, leaf chlorophyll fluorescence, proline content, yield and yield components of sesame cultivars under different moisture regimes. A 4-replicate split plot RCBD field experiment was conducted ...
Read More
Sesame could be an appropriate oilseed crop for water limited environments. This research was aimed at studying growth, leaf chlorophyll fluorescence, proline content, yield and yield components of sesame cultivars under different moisture regimes. A 4-replicate split plot RCBD field experiment was conducted at the Lavark Research Farm, College of Agriculture, Isfahan University of Technology, Isfahan, Iran in 2007. Three levels of irrigation consisting I1 = control (no water deficit), I2 = moderate water deficit and I3 = severe water deficit, representing irrigation after 75, 110 and 145 mm evaporation from the standard Class A Pan, respectively, served as main plots. Four sesame genotypes consisting ‘Non Branching Naz’, ‘Yekta’, ‘Varamin’ and ‘Oltan’ were considered as sub plots. Irrigation regimes and cultivars differed in terms of Fm at grain filling stage, though they did not differ in terms of leaf chlorophyll content and Fv/Fm. The latter trait decreased non-significantly from 0.81 at the I1 to 0.77 at the I3 level of irrigation. Leaf proline content increased from 3.5 at I1 to 8.6 mgg-1 at the I3 level of irrigation. Severe water deficit decreased LAI, pod/plant, seed/pod, grain yield and dry matter by 55, 42, 37, 48 and 49 percent, respectively. Severe water deficit led to a significant decrease in grain yield from 1212 to 625 kg/ha. It seems that water deficit affects sesame growth and grain yield significantly and this negative effect is mainly through a reduction in the photosynthetic surfaces, rather than a decrease in the maximum of quantum efficiency for photosystem II of the present genotypes. Yekta and Varamin sesame were more productive compared to the rest of cultivars.