Mohamad Keshtegar khajedad; Alireza Sirousmehr; Issa Khammari; Khodadad Dahmardeh
Abstract
Water deficit is one of the most important limiting factors of production in crops. The present study was conducted in 2019 to investigate the effect of different irrigation times and foliar application of humic acid on morphophysiological traits and yield of black beans as a split plot in a randomized ...
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Water deficit is one of the most important limiting factors of production in crops. The present study was conducted in 2019 to investigate the effect of different irrigation times and foliar application of humic acid on morphophysiological traits and yield of black beans as a split plot in a randomized complete block design with three replications at Zahak Agricultural Research Station in Sistan and Baluchestan Province. The main factor involves three irrigation treatments, including irrigation from planting to harvest, irrigation from planting to flowering, and irrigation from flowering to harvest, and the secondary factor includes foliar application at four levels of humic acid (0, 1, 2, and 3 per thousand). By increasing drought stress levels, plant height and width, fresh and dry forage yield, leaf area (in both stem and flowering stages), photosynthetic pigments, nitrogen, phosphorus (3.39%), potassium (20.6%), calcium of leaf, grain yield (11.27%), and biological yield (60.22%) has decreased, whereas proline content (31.88%) has increased, compared to the control. Also, by increasing humic acid levels, the values of all studied traits has increased. Humic acid has had a positive effect on black bean plant traits under drought stress conditions, so that its concentration of three per thousand reduces the adverse effects of drought stress, increasing the tolerance of black bean crop. In general, complete irrigation and application of three per thousand humic acids for the production of black beans seems appropriate. It is suggested that in future research, soil application of humic acid consumption should be investigated to compare the efficiency of the methods used.
Raouf Seyed sharif; reza seyed sharifi
Abstract
In order to study the effects of nitrogen starter, putrescine, and nano silicon on yield and some physiological and biochemical traits of rainfed chickpea (Cicer arietinum L.), an experiment was carried out as factorial based on randomized complete block design with three replications in a farm near ...
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In order to study the effects of nitrogen starter, putrescine, and nano silicon on yield and some physiological and biochemical traits of rainfed chickpea (Cicer arietinum L.), an experiment was carried out as factorial based on randomized complete block design with three replications in a farm near Ardabil in 2021. The experiment factors include nitrogen starter (no-nitrogen starter, application of 20 and 40 kg.ha-1 by urea), putrescine foliar aplication (foliar application with water, application of 0.5 and 1 mM putrescine), and nano silicon (foliar application with water, application of 25 and 50 mg.L-1). Means comparision show that the highest level of nano silicon, putrescine, and nitrogen starter have increased chlorophyll a, chlorophyll b, total chlorophyll, and proline content (139%, 137%, 138%, and 80%, respectively), compared to no application of nano silicon, putrescine, and starter nitrogen. The highest level nano silicon and putrescine has increased the activity of catalase, peroxidase enzymes and grain yield (40%, 16.3%, and 16.6%, respectively), compared to no application of nano silicon and putrescine. Maximum quantum yield of photosystem II (7.4%, 8%, and 15.5%, respectively) and the activity of polyphenol oxidase enzyme (17%, 9%, and 21%, respectively) are obtained at the highest level starter nitrogen, putrescine, and nano silicon. It seems that the application of nano silicon, putrescine, and starter nitrogen can increase grain yield of chickpea under rainfed condition as it improves physiological and biochemical traits.
Mojtaba Kaboodkhani; Hadi salek mearaji; Keyvan aghaei; Afshin Tavakoli
Abstract
Quinoa is one of the salinity tolerant plants, capable of playing an important role in providing human food in the future. In order to investigate the effect of salinity stress on physiological traits and yield of quinoa cultivars, a two-factor factorial experiment was conducted as random complete block ...
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Quinoa is one of the salinity tolerant plants, capable of playing an important role in providing human food in the future. In order to investigate the effect of salinity stress on physiological traits and yield of quinoa cultivars, a two-factor factorial experiment was conducted as random complete block design with three replications in 2020 year under greenhouse conditions. Experimental treatments include three quinoa cultivars (Titicaca, Q26, and Giza1) and three salinity levels (0, 15, and30 dS/m). Salinity stress reduced traits such as photosynthetic pigments, relative leaf water content, and grain yield. The chlorophyll a and b content in control conditions, compared to the salinity level of 30 dS/m, have decreased by 46% and 77%, respectively, with the yield dropping by 35.6%, but the decrease in relative water content has been 12.6%. Electrolyte leakage, proline and malondialdehyde content, catalase, and guaiacol peroxidase activity have increased under salinity stress condition. The Q26 cultivar has had the highest content of carotenoids, chlorophyll a, relative water content, soluble proteins, proline, and catalase activity, compared to the others. Titicaca cultivar has had lower malon-dialdehyde (MDA) content and electrolyte leakage than Q26 and Giza1 cultivars, which indicates the least damage to cell membranes, being superior to the other two cultivars. Giza1 cultivar also has had higher chlorophyll b and carotenoids content than the other two cultivars. Q26 and Giza1 cultivars are probably the most resistant and sensitive cultivars to salinity stress, respectively.
farzaneh Pimard; Abdolrahman Mohammadkhani; Ali Niazi; Alireza Shahsavar; Mohammad-Reza Nouri-Emamzadei
Abstract
Drought stress is one of the limiting factors of agriculture in many parts of the world, especially Iran. Understanding the mechanism behind drought stress’ effect on physiological and biochemical processes of genotypes is very useful for selecting and breeding genotypes compatible with Iranian ...
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Drought stress is one of the limiting factors of agriculture in many parts of the world, especially Iran. Understanding the mechanism behind drought stress’ effect on physiological and biochemical processes of genotypes is very useful for selecting and breeding genotypes compatible with Iranian conditions. For this purpose, the present study has been conducted in 2018-2019 in the Biotechnology Research Institute of Shiraz University to investigate the effect of drought stress on physiological and biochemical characteristics of both years’ old pomegranate seedlings of Wonderful cultivar in greenhouse conditions. Irrigation has been carried out at four levels of 100% (control), 75%, 55%, and 35% of field capacity for 50 days. The experiment is based on a completely randomized design with three replications. The results show that drought stress has significantly increased carotenoids, flavonoids, malondialdehyde, and proline. The relative leaf water content, cell membrane stability and anthocyanins has decreased, though there has been no significant difference in chlorophyll and glycine betaine levels between drought treatments. Also, the hydrogen peroxide (81%) and activity of superoxide dismutase (480%), catalase (96%), and ascorbate peroxidase (96%) in 35% of field capacity significantly has increased. According to the results of this study, especially the increase in proline and antioxidant enzymes under drought stress, tolerance mechanisms in pomegranate cultivar Wonderful can be associated with active osmotic regulation and active enzymatic antioxidant system.
Ghorban Khodabin; Zeinolabedin Tahmasebi-Sarvestani; Amir Hossein Shirani rad; Esmaeil Bakhshandeh; Mojdeh Sadat Khayat Moghadam; Shahryar Kazemi; mahsa rafati alashti; ali heidarzadeh
Abstract
In order to study the effects of late-season drought stress and foliar application of zinc sulfate (ZnSO4) and manganese sulfate (MnSO4) on physiological characteristics and yield of rapeseed cultivars, a factorial split-plot test has been conducted in a randomized complete blocks design for two cultivation ...
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In order to study the effects of late-season drought stress and foliar application of zinc sulfate (ZnSO4) and manganese sulfate (MnSO4) on physiological characteristics and yield of rapeseed cultivars, a factorial split-plot test has been conducted in a randomized complete blocks design for two cultivation years (2017-2019) in Karaj, Iran. The experimental treatments include two levels of irrigation (full irrigation (control) and withholding irrigation from flowering stage) and four levels of foliar application (control, ZnSO4, and MnSO4, separately, as well as their combined application) as factorial in the main plots, with rapeseed genotypes (Nima, Niloofar, and KS7) considered as subplots. Combined foliar application of zinc and manganese sulfate under full irrigation conditions increase the content of seed oil and soluble carbohydrates by 3% and 11%, respectively, compared to the control. On the other hand, withholding irrigation from flowering stage increases the percentage of seed oil and leaf proline content, compared to the control by 9% and 5.2%, respectively. Niloofar genotype has had the highest seed yield (5261 kg/ha) under full irrigation conditions, i.e. 9% and 11.8% higher than KS7 and Nima genotypes, respectively. Nima genotype with 3371 kg/ha has had the highest yield in the conditions of withholding irrigation. In general, in both irrigation treatments, simultaneous foliar application of zinc sulfate and manganese has had the greatest impact on the physiological traits of the studied genotypes, thus Niloofar genotype is recommended for full irrigation and Nima genotype for interrupted irrigation.
Mona Arvand; Yousef Sohrabi
Abstract
In order to investigate the effects of integrated management of chemical and biological fertilizers on physiological traits, and grain yield of black cumin (Nigella sativa L.) under different irrigation levels, a split-plot experiment has been conducted based on a randomized complete block design ...
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In order to investigate the effects of integrated management of chemical and biological fertilizers on physiological traits, and grain yield of black cumin (Nigella sativa L.) under different irrigation levels, a split-plot experiment has been conducted based on a randomized complete block design with four replications at research farm University of Kurdistan in the spring of 2016. Two irrigation levels (irrigation at 100% and 70% of the plant water requirement) are randomized on the main plot. Five fertilizer levels (including 100% chemical fertilizers, 80% chemical fertilizers + biofertilizers, 60% chemical fertilizers + biofertilizers, 40% chemical fertilizers + biofertilizers, and non-fertilizer application) are randomized on a sub-plot. The results reveal that under irrigation at 70% of the plant water requirement conditions, proline concentration, soluble carbohydrates concentration, and lipid peroxidation have increased, while the values of other studied physiological parameters as well the grain yield have dropped. The combination of biological and chemical fertilizers positively affect the studied physiological traits and grain yield, such that the highest grain yield belongs to the combined treatment of 60% chemical fertilizers + biofertilizers (798.18 kg ha-1), showing an increase of 37.22% compared to the control (without fertilizer). Due to the positive effects of combining bio-chemical fertilizers on physiological parameters and grain yield, it is recommended to replace the combination of bio-chemical fertilizers instead of employing only chemical fertilizers.
Hamed Narimani; Raouf Seyed sharif; fatemeh aghaei
Abstract
In order to study the effect of foliar and soil application of Zinc on grain weight and some biochemical traits of wheat (Triticum aestivum L.) under soil salinity, an experiment has been conducted as factorial based on randomized complete block design with three replications in research greenhouse of ...
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In order to study the effect of foliar and soil application of Zinc on grain weight and some biochemical traits of wheat (Triticum aestivum L.) under soil salinity, an experiment has been conducted as factorial based on randomized complete block design with three replications in research greenhouse of University of Mohaghegh Ardabili in 2018-2019. Experimental factors include soil salinity levels [control and salinity of 30, 60, and 90 mM] and four methods of zinc application [no zinc as control, soil application zinc as ZnSO4, foliar application nano zinc oxide, and combination of soil and foliar application of zinc]. Results show that both application of ZnSo4 and foliar application nano Zn oxide under 90 mM soil salinity condition increase the catalase and peroxidase enzymes activity, anthocyanin, proline, and soluble sugars content by 20.24%, 17.68%, 13.16%, 32.88%, and 14.08%, respectively, in comparison with no application of zinc under 90 mM soil salinity condition. Also, both soil application of ZnSo4 and foliar application of nano Zn oxide under non-salinity condition decrease hydrogen peroxide and malondialdehyde content in comparison with no application of Zinc under 90 mM soil salinity. Both soil application ZnSo4 and foliar application nano Zn oxide under non-salinity condition has had the highest grain weight (1.016 g per plant), compared to the application of this treatment combination at other salinity levels. It seems that both application of ZnSo4 and nano Zn oxide can increase weight yield of wheat under salinity condition due to their ability in improving biochemical traits.
Mohammad Sayyari; Monirea Moradi Farsa; Ali Azizi
Abstract
The phonological stage in which the abotic stress occurs is effective in determining the content of secondary metabolites in medicinal plants. This research deals with the effects of imposing drought stress at different phenological stages on growth and some physiological and phytochemical responses ...
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The phonological stage in which the abotic stress occurs is effective in determining the content of secondary metabolites in medicinal plants. This research deals with the effects of imposing drought stress at different phenological stages on growth and some physiological and phytochemical responses of Nepeta crispa L as a split plot design in Bu-Ali Sina University during 2019. Drought stress at three levels, including mild, moderate, and severe stress (with 80%, 60%, and 40% of field capacity, respectively) has been applied at two phenological stages (seedling establishment stage with 6 true leaves and pre-flowering stages). Results from statistical analysis show that drought stress has had a significant effect on all evaluated growth traits and phytochemical parameters of the plant including proline content, relative water content (RWC) of leaves, total phenols, flavonoids, tannins, antioxidant activities, and phenolic acids. Imposing drought stress at both phenological stages reduce plant growth, decreasing chlorophyll content and RWC. However, production of phenolic compounds and phenolic acids (including rosmarinic, chlorogenic and caffeic acids) at high level and accumulation of proline through imposing drought stress reduce the adverse effects of stress and increase the medicinal properties of the Nepeta crispa. Also, the effect of drought stress imposing at pre-flowering stage has been greater than its application in the seedling establishment stage. According to the results, it is recommended to impose moderate drought stress at pre-flowering stage to increase biosynthesis of some phytochemical compounds in Nepeta crispa medicinal plants.
fereshteh darabi; Nosratollah Abbasi; Mohammad Javad Zarea
Abstract
This paper aims at evaluating the effects of putrescine and 24-epibrasinolide on altering the activity of antioxidant enzymes to tolerate drought stress in basil. The plan has been implemented as factorial split plot layout based on a randomized complete block design with three replications in the cropping ...
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This paper aims at evaluating the effects of putrescine and 24-epibrasinolide on altering the activity of antioxidant enzymes to tolerate drought stress in basil. The plan has been implemented as factorial split plot layout based on a randomized complete block design with three replications in the cropping years 2017-2018 and 2018-2019 at the research farm of the Faculty of Agriculture, Ilam University. Experimental treatments include drought stress at three levels (40, 80, and 120 mm evaporation from Class A evaporation pan) as the main plots, putrescine foliar application at three levels (0, 0.5, and 2 (mM) and 24- Epibrassinolid foliar application at three levels (0, 0.5, and 2 μM) as subplots. Results show that the highest amount of proline in 120 mm occurs during the first year of the experiment and the use of putricin (2 mM) and 24-epibrasinolide (0.5 μM) also cause a further increase in proline. The highest amount of catalase has been observed in the 120 mm evaporation treatment and the application of 2 mM putrescine. The interaction of drought stress, putrescine, and 24-epiprasinolide also show that the highest levels of ascorbate peroxidase and superoxide dismutase belong to the treatment of 120 mM where the highest concentrations of putrescine (2 mM) and 24-epibrasinolide (2 μM) is observed. Under optimal irrigation conditions (40 mm), the combined application of lower concentrations of putrescine (0.5 mM) and epibrasinolide (0.5 μM) and under drought stress conditions (80 and 120 mm), higher concentrations of these two substances (2 mM putrescine and 2 μM epibrasinolide) increase the enzyme guaiacol peroxidase. Multiple interactions of year, drought stress, putrescine, and 24-epibrasinolide have had a significant effect on total flavonoids and essential oil percentage of basil. In both experimental years, application of 2 mM putrescine and 2 μM 24-epibrasinolide at all levels of drought stress 120 mm cause a further increase in these two traits which has been greater in the first year than the second one. Concomitant use of 2 mM putrescine and 2 μM epibracinolide due to increased proline synthesis and antioxidant enzymes is the best treatment to reduce the effects of drought stress in basil, indicating a synergistic relation between the two. The substance boosts the growth of basil, increasing the percentage of its essence.
Mohamad reza Kimiaei; Alireza Sirousmehr; Barat ALI Fakheri
Abstract
In order to investigate the effects of irrigation regimes and foliar silicon spraying on quantitative and physiological characteristics of borage (Borago officinalis), an experiment is conducted as split plot based on randomized complete block design with three replications during 2014 at the University ...
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In order to investigate the effects of irrigation regimes and foliar silicon spraying on quantitative and physiological characteristics of borage (Borago officinalis), an experiment is conducted as split plot based on randomized complete block design with three replications during 2014 at the University of Zabol research farm in Zahak. Treatments include irrigation regimes at three levels; (90%, 70%, and 50% FC) comprising the main-treatments and four levels of silicon sprying including spraying in the form of sodium silicate (Na2SiO3) (0, 2, 4, and 6 mM) as sub-treatments. The results indicate that the highest amount of proline is obtained from the interaction of irrigation regime with 50% of field capacity, without the use of silicon at the rate of 0.7847 mg.g-1. Based on the results of average comparison, the highest amount of chlorophyll a (15.919 mg/g fresh weight), chlorophyll b (7.14 mg/g fresh weight), from the interaction of irrigation regime 70% of field capacity and application of 2 mM silicon, as well as number of flowers per plant, total dry yield of plant (520 kg.ha-1) and biological yield (1187 520 kg.ha-1) Per hectare), 70% and 50% of field capacity and application of 6 mM silicon are obtained from the interaction effect of irrigation regime, respectively. Overall, the results suggest that silicon application mitigate drought stress impact and led to increasing drought tolerance of borage. Since flower and flowering branches of plant has economic value and application of 4 mM silicon acid is recommended to obtain the greatest flower yield under drought stress conditions.
Aliyeh Shafiei; Mehdi Hadadinejad; Saeed eshghi; kamran ghasemi
Abstract
The present research tries to investigate the effect of potassium silicate and mycorrhizal fungus on heat stress tolerance of strawberries and evaluate the possibility of increasing plant tolerance to heat at research greenhouse in Sari Agriculture and Natural Resources University at 2019. It is in a ...
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The present research tries to investigate the effect of potassium silicate and mycorrhizal fungus on heat stress tolerance of strawberries and evaluate the possibility of increasing plant tolerance to heat at research greenhouse in Sari Agriculture and Natural Resources University at 2019. It is in a completely randomized factorial design with three factors including two levels of mycorrhizal fungi (presence and absence of mycorrhizal fungi), three levels of potassium silicate (0, 50 and 100 mg.l-1) spray in heat stress (25 and 41 °C) with three replications. The results show that application of potassium silicate reduces the Photosynthtic active radiation (PAR) damage in strawberry leaves, which PAR of leaves bottom reduce three folds in comparison to the control, when the PARtop is 1133 umol.m2.s-1. In contrast, treatment of the plant with potassium silicate in the presence of mycorrhiza prevented increases transpiration and stomatal conductivity for water cooling the leaves during heat stress. Inoculation of the roots with mycorrhiza fungi leads to a wide network of hyphae in root uptake, improving water use efficiency by up to 72%, which in turn results in increaseed net photosynthesis. However, heat stress increases minimum fluorescence and reduces maximal quantum efficiency of photosystem II. But the simultaneous application of mycorrhiza and potassium silicate is able to improve this index to a good level (0.76). Finally, it has been found that high temperature stress affects many photosynthetic factors of strawberry plant that the use of potassium silicate in combination with mycorrhizal fungus, despite their separate use, to a large extent prevents heat damage to different parts of the plant.
omid Lotfifar; samaneh Mottaghi
Abstract
The purpose of this study is to use meta-analysis to combine the results of experiments conducted in Iran on the effect of drought stress due to irrigation cessation at different stages of development, on yield and yield components of canola. The studied traits include grain yield, oil and dry matter, ...
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The purpose of this study is to use meta-analysis to combine the results of experiments conducted in Iran on the effect of drought stress due to irrigation cessation at different stages of development, on yield and yield components of canola. The studied traits include grain yield, oil and dry matter, number of pods per plant, number of seeds per pod, 1000-seed weight, and grain oil content. Results show that all traits under the influence of stress at different stages of development are significantly affected by drought stress. Irrigation interruption in different stages on average cuts grain yield by 34.5%, oil yield by 36%, oil content by 4.7%, dry matter by 25.8%, number of pods by 35.4%, seeds per pod ratio by 18.5%, and 1000-seed weight by 17.5%, while raising the proline content by 49.9%. The highest reduction rate in grain and oil yield belongs to the irrigation cut-off treatment, in turn leading to a reduction of 41% and 46% of these two traits, respectively. Also, regarding the yield components, the highest reduction in the number of pods per plant, number of seeds per pod, and 1000-seed weight is observed in the cut-off treatments of stem, flowering, and podding, respectively.
Mojdeh Sadat Khayat Moghadam; Ahmad Gholami; Amir Hossein Shirani rad; Mahdi BaradaranFiroozabadi; Hamid Abbasdokht
Abstract
In order to study the effects of late-season drought stress and foliar application of potassium silicate on yield and yield components of spring genotypes of canola, a factorial split-plot experiment is conducted in Karaj, Iran in a randomized complete block configuration with three replications for ...
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In order to study the effects of late-season drought stress and foliar application of potassium silicate on yield and yield components of spring genotypes of canola, a factorial split-plot experiment is conducted in Karaj, Iran in a randomized complete block configuration with three replications for two years of cultivation (2016-2018). The irrigation is performed at two levels in this study, including routine irrigation (control) and interruption of irrigation from the pod formation stage. Potassium silicate foliar application at two levels is comprised of 0 and 4 g liter-1 in factorial status in main plots and five Brassica napus L. genotypes including OG×AL, RGS×SLM, DALGAN, RGS003 and RGS×Okapi in subplots. The results show that the measured properties are affected by the applied treatments. Full irrigation with foliar application in OG×AL genotype results in highest seed yield (5620 Kg/ha) and total chlorophyll content (1.71 mg/g.FW) increase stomatal resistance, leaf proline, leaf soluble and carbohydrates, decreasing total chlorophyll content and relative leaf water content. Under drought stress conditions, DALGAN and RGS× SLM genotypes have higher yield, which indicates its better performance under stress conditions. Finally, our research demonstrates Potassium Silicate's beneficial effects in improving the drought tolerance of canola plants, particularly at the end of the season. Our study will act as a foundation for any attempt in new approaches to mitigate drought damage, establishing a functional connection between the position of potassium silicate, physiological response, and drought stress tolerance in canola plants.
Siavash Heshmati; Gholam Akbari; elias soltani; Majid Amini Dehaghi; Kayvan Fathi Amirkhiz; Keyvan Maleki
Abstract
In order to study the foliar application of melatonin on plants grown from safflower in different seed qualities under drought condition, two field experiments have been carried out at research farm of Aburaihan Campus, University of Tehran, between 2017 and 2018 growing seasons. The experimental design ...
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In order to study the foliar application of melatonin on plants grown from safflower in different seed qualities under drought condition, two field experiments have been carried out at research farm of Aburaihan Campus, University of Tehran, between 2017 and 2018 growing seasons. The experimental design is split-factorial in a randomized complete block design with four replicates, with the treatments being consisted of two levels of drought stress (1- normal irrigation (no-stress) and 2- irrigation after reaching 85% of soil moisture depletion of field capacity at flowering stage (drought stress)), in the main plots. The subplots include 4 treatments, including a factorial combination of seed quality (stored seed and recently harvested seeds) and foliar application (the control and melatonin foliar application). Results from these experiments illustrate that melatonin foliar application has significantly increased the seed yield of stored seed by 589Kg.ha-1, compared to the control. It is also shown that SOD and CAT activities have been increased in response to melatonin in plants grown from recently-harvested seeds under drought condition. Furthermore, it is indicated that foliar application of melatonin decrease the amount of leaf soluble proteins by 28% in plants grown from recently-harvested seeds under drought conditions. According to the results, the amount of malondialdehyde is decreased by 37% in plants grown from recently-harvested seeds by foliar application of melatonin under drought conditions. It seems that seed quality has an enormous influence on grown plants and that using melatonin may play a substantial role in ameliorating the injuries derived from stress on safflower plant.
Sevda Ghasemi Germi; morteza Barmaki; salim farzaneh; Mandana Amiri
Abstract
AbstractThis study aims at investigating the morpho-physiological responses of potato superelite mini-tubers to different coating treatments factorial experiment, based on randomized complete block design with three replications in Research Farm of Mohaghegh Ardabili University between 2018 and 2019. ...
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AbstractThis study aims at investigating the morpho-physiological responses of potato superelite mini-tubers to different coating treatments factorial experiment, based on randomized complete block design with three replications in Research Farm of Mohaghegh Ardabili University between 2018 and 2019. Experimental factors at four levels include mini-tubers filler (zeolite and cocopeat), superphosphate (soil and pellet application), chitosan, and superabsorbent with control treatment (without pelleting). Application of cocopeat with superphosphate leads to the highest average number of tubers per plant (8.36), fresh and dry tuber yield (18.23 and 3.88 tons per hectare), and biological yield (21.69 tons per hectare). The use of chitosan in mini-tuber pelleting decrease the activity of catalase and peroxidase enzymes (19.67% and 16.37%, respectively), increasing the activity of polyphenol oxidase (37.80%). Zeolite increase and cocopeat decrease the tuber protein percentage, compared to the control. This increase in protein percentage with increasing tuber yield leads to a 32.23% increase in tuber protein yield in zeolite application, compared to control treatment. The use of superphosphate in mini-tuber pelleting also increase the protein percentage, thus leading to a 30.30% increase in protein yield, compared to control mini-tuber. In principal component analysis (PCA), the first component, with which 35% of the changes could be explained, include the yield of protein, lysine, and methionine, and the second component, with which 25% of the changes could be explained, include the protein, methionine, lysine, and total nitrogen. Finally, the results show that the mini-tuber pelleting by superphosphate, zeolite, and superabsorbent have had significant additive effects on yield and quality traits.
Mahdi Motaefi; Alireza Sirousmehr; Mohsen Mohsen Mousavi nik
Abstract
Drought stress is the most important factor in crop yield reduction. It is important to know how to cope with drought. This experiment is carried out to investigate the effect of organic growth stimulator (Hamoon green) on yield and some physiological indices of safflower under drought stress, during ...
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Drought stress is the most important factor in crop yield reduction. It is important to know how to cope with drought. This experiment is carried out to investigate the effect of organic growth stimulator (Hamoon green) on yield and some physiological indices of safflower under drought stress, during 2017-2018 in university of Zabol (Chahnime) as a split plot, based on RCBD with three replications. Experimental treatments include drought factor based on plant growth stages at three levels (complete irrigation throughout the growing season, irrigation up to terminal bud forms, and irrigation up to completed flowering) and spraying factor of organic growth stimulator at 4 levels (pure water), the foliar application has been Hamoon green 1 liter in 10 liters + calcium, Hamoon green 1 liter in 20 liters + calcium and Hamoon green 1 liter in 30 liters+ calcium. Results show that drought has reduced the height, 1000-seed weight, and oil percentage, by 44.41%, 31.24%, and 17.10%, respectively. Spraying hamoon green 1 liter in 10 liters+ calcium leads to an increase of 18.03%, 13.90%, and 13.74% for the above traits, compared to the control. The highest grain yield (1882.55 kg.ha-1), chlorophyll a, b, total and carotenoids (3.49, 0.94, 4.44, and 7.37 mg.gr-1 fw, respectively), selenium content (5.18 mg.kg-1 fw) and seed calcium content (2.91%) are obtained from hamoon green 1 liter in 10 liter + calcium and complete irrigation treatment. In general, it seems that by spraying 1 liter per 10 liters+ calcium, a good performance can be achieved.
Atefeh Sadat Mousavi; Masoumeh Naeemi; ali Rahemi karizaki; Abdolatif Gholizadeh
Abstract
In order to investigate the effect of chitosan and salicylic acid elicitors on osmotic adjustment and some physiological traits of Hyssop under experimental drought conditions, an experiment has been carried out as a split plot design with randomized complete block design with three replications at research ...
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In order to investigate the effect of chitosan and salicylic acid elicitors on osmotic adjustment and some physiological traits of Hyssop under experimental drought conditions, an experiment has been carried out as a split plot design with randomized complete block design with three replications at research farm of Gonbad Kavous University during 2018-2019. Irrigation factor at three levels include 7, 14, and 21 days irrigation interval as main factor and four levels of spraying including no elicitor (control treatment, spraying), salicylic acid (300 mg. l-1), Chitosan spraying (5 g. l-1), and combination of salicylic acid and chitosan, considered as auxiliary agents. Results of analysis of variance show that the interaction between spraying and irrigation has had significant effects on ion leakage, chlorophyll a content, soluble sugar, and malondialdehyde content but insignificant impacts on carotenoid and essential oil yield. Spraying of salicylic acid, with 17% reduction in ion leakage and 40% reduction in plant malondialdehyde, compared to the control, increase the stability of cell membranes under 21-day irrigation stress. In the same treatment, chitosan has increased the amount of photosynthetic pigments and total chlorophyll by 51%, compared to the control. According to the results, salicylic acid and Chitosan have been able to increase the ability of hyssop in response to water deficit stress by activating different tolerance mechanisms.
sahar keramati; Ahmad Gholami; Mehdi Baradaran; Hamid Abbasdokht
Abstract
In order to investigate the role of foliar application of yeast extract on physiological and Biochemical properties of cowpea (Vigna sinensis L.) under drought stress conditions, a factorial experiment has been conducted as a randomized complete block design with three replications in Agriculture Campus ...
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In order to investigate the role of foliar application of yeast extract on physiological and Biochemical properties of cowpea (Vigna sinensis L.) under drought stress conditions, a factorial experiment has been conducted as a randomized complete block design with three replications in Agriculture Campus of Shahrood University of Technology (located in Bastam) during 2017 under drought stress and foliar application of yeast extract. Drought stress involves two stages of 50 present flowering and 50 present pod formation with control treatment (without irrigation interruption). Foliar application of yeast extract (two, four, six g/L, and control) are repeated twice with a seven-day interval, with the first one being 30 days after planting. Results show that drought stress has decreased relative water content of leaves. In contrast, relative water content (5.82%), proline content (30.74%), leaf flavonoid (83%), nitrogen (3.82%), phosphorus (22.22%), and potassium (43%) are among the traits that have increased with the use of yeast extract, compared to the control. Six g / L of the yeast extract has increased grain yield (1.02 t ha-1), leaf anthocyanin (0.072 mg /g fresh weight), and proline (31.62 mg /g fresh weight). It can be said that a concentration of six g/L of yeast extract can be useful to reduce the severity of drought stress. The yeast extract can act as an ecological strategy to improve the defensive response against environmental stresses.
Seyedahmad Kalantarahmadi; Jahanfar Daneshian
Abstract
The present experiment has been carried out as a split plot based on randomized complete block design with three replications in Safiabad Agricultural and Natural Resources Research and Education Center of Dezful between 2012 and 2014. The main plots is consisted of 3 sowing dates (November 5, November ...
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The present experiment has been carried out as a split plot based on randomized complete block design with three replications in Safiabad Agricultural and Natural Resources Research and Education Center of Dezful between 2012 and 2014. The main plots is consisted of 3 sowing dates (November 5, November 25, and December 15) with the sub plots including 10 levels of foliar application (100, 200, 300 mg.lit-1 ascorbic acid, 100, 200, 300 μmol ascorbic acid, 10, 20, 30 vol% methanol, and the foliar application of distilled water as control treatment). The effect of sowing date and foliar application as well as their interaction has had significant effect on proline, soluble sugars, number of siliques per plant, and seed yield (P≤0.01). Comparing means of sowing date × foliar application shows that the maximum number of siliques per plant (68.96) has been formed in the first sowing date with the foliar application of ascorbic acid (300 mg.lit-1). There has been a significant difference between different sowing dates: the seed yield has decreased in the second and third sowing date, compared to the first one by 145% and 30%, respectively. However, foliar application of experimental treatments has increased the seed yield in all sowing dates, but it is recommended to use ascorbic acid (300 mg.lit-1) in the first and third sowing dates as well as salicylic acid (200 µmol) and methanol (10%) in the second sowing date.
Saeid Davazdahemami; Shekofe Enteshari; Marzyeh Allahdadi; shahram yasmani
Abstract
In order to investigate the effect of irrigation water salinity on some characteristics of Ajowan (Carum copticum L. C.B. Clarke), a field experiment has been conducted in a completely randomized design with 3 replications in Isfahan, Iran during 2013. The treatments involve different levels of saline ...
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In order to investigate the effect of irrigation water salinity on some characteristics of Ajowan (Carum copticum L. C.B. Clarke), a field experiment has been conducted in a completely randomized design with 3 replications in Isfahan, Iran during 2013. The treatments involve different levels of saline water, namely 2.5 (control), 6, 9, and 18 dS.m-1. The determined traits include the yield, biochemical parameters, mineral contents, and seed essential oil content and quality. Results show that increasing salinity decreases biological yield and seed yield. Changes in essential oil components, caused by salinity, have been low, showing no specific trend. The major compound in the seed essential oil of C. capticum is thymol (56.1% to 61.2% of the essential oil). The highest concentration of total protein (root: 3.6 and shoot: 8.2 mg g-1 DW) is assigned to the control treatment, dropping significantly as salinity levels rise. Increasing salinity enhances the amount of proline and reducing sugars so that the highest amount of root proline, equal to 12 mg g-1 FW, and reducing sugars (root: 30.5 and shoot: 62 mg g-1 DW) comes from salinity of 18 dS.m-1. Increasing salinity levels raises the amount of phenolic compounds in the shoot, though this increase has not been considerable. The treatment of 18 dS.m-1 has had the lowest concentration of K+ (root: 5 and shoot: 22 mg g-1 DW) and the highest concentration of Na+ (root: 54 and shoot: 64 mg g-1 DW).It can be concluded that by increasing salinity levels, the amount of resistant osmolytes rises.
Mahdieh Fallah; Hashem Hadi; Reza Amirnia; Abdollah Hasanzadeh Gorttapeh
Abstract
The present experiment has been conducted as a factorial based on randomized complete block design with three replications in the Agricultural and Natural Resources Research farm of Urmia for two years (2017-2018) to investigate the effect of green manure residues and fertilizer sources on physiological ...
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The present experiment has been conducted as a factorial based on randomized complete block design with three replications in the Agricultural and Natural Resources Research farm of Urmia for two years (2017-2018) to investigate the effect of green manure residues and fertilizer sources on physiological traits, biological yield, and seed oil content of linseed. Experimental factors include green manure in two levels: no green manure and red clover (Trifolium pratense), different sources of fertilizer in four levels: no fertilizer, mycorrhiza, vermicompost and mycorrhiza + vermicompost, both in full irrigation condition and irrigation terminationat the flowering stage. Results of the combined analysis show that the year has a significant effect on chlorophyll a, colonization percentage, grain nitrogen and phosphorus, biological yield, and seed oil. Irrigation termination treatment decreases all traits except proline and soluble carbohydrate contents. Green manure, mycorrhizae, and vermicompost significantly increase the studied traits at both irrigation levels, except for grain nitrogen, not affected by green manure. The application of mycorrhiza+ vermicompost, with or without green manure, is more effective than their individual applications. The dual application results in an increase of 68.98% and 61.34% for biological yield and 11.8% and 4.5% for seed oil, compared to the control in complete irrigation treatment, respectively. In general, green manure, mycorrhiza, and vermicompost can improve the quantitative and qualitative performance of flaxseed in irrigation terminationat the flowering stage.
jaber khordadi Varamin; Farzad Fanoodi; jafar masoud sinaki; Shahram rezvan; Ali Damavandi
Abstract
To investigate the physiological responses of sesame cultivars to the application of nano magnesium and chitosan biopolymer under different irrigation regimes, a split factorial based on randomized complete block design has been conducted with three replicates between 2017 and 2018. The irrigation cut-off, ...
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To investigate the physiological responses of sesame cultivars to the application of nano magnesium and chitosan biopolymer under different irrigation regimes, a split factorial based on randomized complete block design has been conducted with three replicates between 2017 and 2018. The irrigation cut-off, based on BBCH scale, has served as the main factor (normal irrigation, irrigation up to 50% flowering, and seed ripening), with the sub factors including Oltan and Dashtestan-2 sesame cultivars, and nano magnesium (application and non-application) and chitosan (control, 4.8, and 6.4 g.L-1). The highest mean grain yield belongs to the application of 6.4 g.L-1 chitosan under normal irrigation with an average of 1235.1 kg.ha-1. Also, the highest total chlorophyll content is observed in Dashtestan-2 genotype under normal irrigation with a mean of 24.7 mg.g-1 FW and the lowest mean have been obtained in both genotypes under irrigation up to 65 BBCH with a mean of 17.21 and 17.46 mg.g-1 FW, respectively. Application of nano fertilizer in Oltan genotype under irrigation up to 65 BBCH increases the catalase activity by 41.11%, compared to the control treatment. The highest activity of ascorbate peroxidase has been achieved when not applying chitosan under irrigation conditions up to 65 BBCH. It has risen by 55.06%, compared to the control treatment. In general, the results show that irrigation up to 65 BBCH reduces grain yield, in turn alleviated by the negative effects of stress on magnesium and chitosan nanoparticles (9.93% and 27.46%, respectively). Based on the regression analysis results, four traits, namely chlorophyll b, total chlorophyll, proline, and catalase, enter the model that explains 42.11% of the total grain yield variations. Results of simple correlation between traits and regression analysis indicate the indirect effects of physiological traits on grain yield and among the studied parameters, photosynthetic pigments has been of high account in stress conditions.
Ali Nakhzari Moghaddam; Hajberdi Ghelichi Yanghagh; Abbas Biabani; Fakhtak Taliey
Abstract
In order to study the effect of nitrogen and irrigation interval on quantity traits and protein of mung bean (Vigna radiata L.), a factorial experiment based on randomized complete block design was conducted with three replications at research farm of Gonbad Kavous University during 2016. Interval irrigation ...
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In order to study the effect of nitrogen and irrigation interval on quantity traits and protein of mung bean (Vigna radiata L.), a factorial experiment based on randomized complete block design was conducted with three replications at research farm of Gonbad Kavous University during 2016. Interval irrigation factor was at three levels including irrigation each 10, 20 and 30-day, nitrogen fertilizer in three levels of non-application and application of 50 and 100 kg per hectare and mung bean genotypes in two levels of VC-1973A and Partov. The results showed that the highest grain yield was obtained from 30-day irrigation interval with 100 and 50 kg per hectare nitrogen and the lowest grain yield was obtained from 10–day irrigation interval and 100 kg nitrogen/ha and non-application treatment and also 20–day irrigation interval and non-application of nitrogen/ha. Grain yield in VC-1973A was 72.52% higher than partov gernotype. The highest protein percentage was obtained from irrigation interval of 30-day and the lowest was obtained from 10-day irrigation interval. With increasing of nitrogen consumption, protein percentage increased but proline decreased. Increasing of irrigation intervals from 10 to 30-day increased the amount of proline in mung bean genotypes. Protein percentage and amount of proline in Partov was 9.49% and 29.66% more than VC-1973A. In addition, although in some traits Partov was superior but in more traits especially grain yield VC-1973A was better than Partov and increasing of interval irrigation and nitrogen consumption created better conditions for mung bean production.
Tahereh Sedighi Shiri; Malek Hossein Shahriari; Mohammad Hedayat; Shahriar Osfouri
Abstract
In order to study the effect of organic amendment application on the growth and biochemical characteristics of French marigold (Tagetes patula) in soil contaminated with different levels of gas condensate, a factorial experiment was conducted based on a completely randomized design with three replications. ...
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In order to study the effect of organic amendment application on the growth and biochemical characteristics of French marigold (Tagetes patula) in soil contaminated with different levels of gas condensate, a factorial experiment was conducted based on a completely randomized design with three replications. The experimental factors consisted of gas condensate at five levels of 0, 7,500, 15,000, 30,000 and 60,000 μL per kg of soil, and soil amendment including vermicompost (5%), biochar (2%), Activated carbon (1%), vermicompost+ activated carbon+ biochar and non-amendment treatments. The results of the analysis of variance showed that the main and interactive effects of gas condensate and soil amendments were significant on traits of fresh and dry weight of root and shoot, chlorophyll a and b and proline content (P < 0.01). At the highest level of contaminant, the application of activated carbon and vermicompost+ activated carbon+ biochar respectively caused 3.82 and 4.45-fold increase in shoot fresh weight, 3.76 and 4.4-fold increase in root fresh weight, 2.52 and 2.56-fold increase in chlorophyll a, and also decreased 30.66 and 39.5 percent of proline content compared to the non-amendment treatment at this level of contaminant. The results of this research indicated the effective and useful role of organic soil amendment, especially activated carbon and vermicompost+ activated carbon+ biochar in reducing the toxicity of gas condensate on French marigold.
Khiermohammad Mohammadi; Ali Ebadi; Mohammad Ali Askari Sarchishmeh; Mohammad Reza fattahi Moghaddam; Hossein Hokmabadi
Abstract
Plants employ antioxidant mechanism to face different stresses. In this research work the effect of salinity stress, caused by increasing sodium chloride, on the antioxidative system and some physiological characteristics of selected pistachio seedlings (Badami-Riz-e- Zarand, Qazvini, Sarakhs, Atlantica ...
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Plants employ antioxidant mechanism to face different stresses. In this research work the effect of salinity stress, caused by increasing sodium chloride, on the antioxidative system and some physiological characteristics of selected pistachio seedlings (Badami-Riz-e- Zarand, Qazvini, Sarakhs, Atlantica UCB1, and Hybrids of Pistacia. vera cv. Badami-Riz-e-Zarand × P. Integerrima, P. vera cv. Sharakhs× P. Integerrima, P. atlantica Desf. × P. Integerrima ) was studied. A greenhouse experiment was conducted as a factorial based on a completely randomized design with three replications in Cocopit and Perlite media. Treatments consisted of four salinity levels (0, 75, 150 and 225 mM NaCl) and above mentioned Root stocks. Using salt in nutrient solution was carried out in the second year of seedling growth for nine weeks. At the end of experiment, some physiological and biochemical traits of seedlings were measured. Results showed that, leaf area and total chlorophyll contents decreased in all treated plants. Under the salinity stress, a higher reduction in the observed traits was noticed in the Atlantica rootstock while less effect on the Badami-Riz-e-Zarand was noticed. Also, it has been found that by increasing salinity levels the levels of proline, total phenol content and antioxidant enzymes activity were higher in Badami-Riz-e-Zarand × P. Integerrima, Badami-Riz-Zarand and Qazvini compared to others. It seems that Hybrid of Badami-Riz-e-Zarand × P. Integerrima, Badami-Riz-Zarand and Qazvini rootstocks had more ability to sustain leaf water and proteins and therefore, were more resistant to salinity stress.