Maryam Tatari; Seyed Asghar Mousavi
Abstract
According to problems caused by application of non-uniform seedling rootstocks in stone fruit treesorchards, the use of uniform and consistent rootstocks with these trees is essential. For determination ofthe best culture medium, explants of apical and lateral buds of Tetra, Nemaguard and GF677 rootstockswere ...
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According to problems caused by application of non-uniform seedling rootstocks in stone fruit treesorchards, the use of uniform and consistent rootstocks with these trees is essential. For determination ofthe best culture medium, explants of apical and lateral buds of Tetra, Nemaguard and GF677 rootstockswere prepared. Explants were placed in modified MS, WPM and Knop media each containing 0.6 mg/lBAP and 0.01mg/l NAA in mid-Spring and after disinfection. This research was carried out as factorialexperiment in a completely randomized design with four replicates and four observations per eachreplication. Tetra, GF677 and Nemaguard rootstocks produced the highest of plantlet numberrespectively. Nemaguard rootstock did not show any proliferation in Knop medium and Nemaguardexplants showed chlorosis on this medium. This medium caused chlorosis in produced plantlets of Tetraexplants. Explants of every three rootstocks were produced the highest plantlet number and length in MSmodified medium. Therefore, combinations of plant growth regulators were evaluated in this medium.Three different combinations were evaluated. Among different combinations of plant growth regulators(0.8 and 1 mg/l BAP and 0.1 and 0.5 mg/l NAA), the highest plantlet number and length were producedin MS medium containing 0.6mg/l BAP and 0.01mg/l NAA. Increase of plant growth regulatorsconcentrations led to vitrification and callus production in GF677 rootstock. The highest root number androoting percent were achieved in modified MS medium containing 1 mg/l NAA and explants of Tetrarootstock produced the highest root number and length.
Maryam Tatari; Asghar Mousavi; Parisa Mashayekhi
Abstract
For vegetative propagation of Nemaguard, St Julien A, Tetra and GF677 rootstocks via cutting, two separate experiments were carried out in a factorial based on completely randomized design. Semi hardwood cuttings were taken in late August and then treated with concentrations of zero, 1500, 2000, 2500 ...
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For vegetative propagation of Nemaguard, St Julien A, Tetra and GF677 rootstocks via cutting, two separate experiments were carried out in a factorial based on completely randomized design. Semi hardwood cuttings were taken in late August and then treated with concentrations of zero, 1500, 2000, 2500 and 3000 mg/l IBA. Cuttings were cultured in perlite medium and in a greenhouse with mist system. In December, hardwood cuttings were taken and treated with concentrations of zero, 2500, 3000, 3500 and 4000 mg/l IBA. Cuttings were cultured in greenhouse that equipped with bottom heat. After two months, the percentage of rooting, root number and root length were recorded. The results showed that semi hardwood cuttings of GF677 and then Nemaguard and Tetra had the highest root number and the most rooting percentage. Root length of these three rootstocks was not significantly different from each other. St Julien A showed the lowest rooting. Most appropriate concentrations for semi hard wood cutting was 2000, 2500 and 3000 mg/l IBA. Hardwood cuttings of GF677 in 4000 mg/l IBA treatments produced the most number of roots and the highest rooting percentage. Hardwood cuttings of Tetra did not produce any roots. Rooting percentage of semi-hardwood cuttings was higher than hardwood cuttings. In general, application of semi-hard wood cutting of these rootstocks and treatment of those with concentrations of 2000 and 2500 mg/l IBA is advisable.